The green seaweed Ulva: tomorrow's "wheat of the sea" in foods, feeds, nutrition, and biomaterials.

Ulva, a genus of green macroalgae commonly known as sea lettuce, has long been recognized for its nutritional benefits for food and feed. As the demand for sustainable food and feed sources continues to grow, so does the interest in alternative, plant-based protein sources. With its abundance along coastal waters and high protein content, Ulva spp. have emerged as promising candidates. While the use of Ulva in food and feed has its challenges, the utilization of Ulva in other industries, including in biomaterials, biostimulants, and biorefineries, has been growing. This review aims to provide a comprehensive overview of the current status, challenges and opportunities associated with using Ulva in food, feed, and beyond. Drawing on the expertise of leading researchers and industry professionals, it explores the latest knowledge on Ulva's nutritional value, processing methods, and potential benefits for human nutrition, aquaculture feeds, terrestrial feeds, biomaterials, biostimulants and biorefineries. In addition, it examines the economic feasibility of incorporating Ulva into aquafeed. Through its comprehensive and insightful analysis, including a critical review of the challenges and future research needs, this review will be a valuable resource for anyone interested in sustainable aquaculture and Ulva's role in food, feed, biomaterials, biostimulants and beyond.

The contribution of eye gaze and movement kinematics to the expression and identification of social intention in object-directed motor actions.

The intention to include another person in an interaction (i.e., social intention) is known to influence the spatio-temporal characteristics of motor performances. However, the interplay between these kinematic variations and the social cues provided by eye gaze has not been properly assessed yet. In the present study, we tested whether limiting the access to eye gaze altered the motor-related effects of social intention on motor performances. In a dyadic interaction, the agents' task was to displace a dummy glass to a new position with the intention to fill it themselves (personal intention) or having it filled by the observers facing them (social intention). The observers performed their action only when they were able to identify a social intention in agents' action. The task was performed while having access to observers' eye gaze or not, through the manipulation of an occluder. Results showed an effect of social intention on agents' motor performances, that induced an amplification of the kinematic spatio-temporal parameters. Such amplification was smaller when the observers' eye gaze was not available. In this latter condition, the identification of the social intention in the observed actions was impaired. Altogether, the results suggest that the presence of eye gaze cues contributes significantly to the success of social interaction, by facilitating the expression and the understanding of social intentions through the kinematics of object-directed actions.

The Neurocognitive Bases of Meaningful Intransitive Gestures: A Systematic Review and Meta-analysis of Neuropsychological Studies.

Researchers and clinicians have long used meaningful intransitive (i.e., not tool-related; MFI) gestures to assess apraxia-a complex and frequent motor-cognitive disorder. Nevertheless, the neurocognitive bases of these gestures remain incompletely understood. Models of apraxia have assumed that meaningful intransitive gestures depend on either long-term memory (i.e., semantic memory and action lexicons) stored in the left hemisphere, or social cognition and the right hemisphere. This meta-analysis of 42 studies reports the performance of 2659 patients with either left or right hemisphere damage in tests of meaningful intransitive gestures, as compared to other gestures (i.e., MFT or meaningful transitive and MLI or meaningless intransitive) and cognitive tests. The key findings are as follows: (1) deficits of meaningful intransitive gestures are more frequent and severe after left than right hemisphere lesions, but they have been reported in both groups; (2) we found a transitivity effect in patients with lesions of the left hemisphere (i.e., meaningful transitive gestures more difficult than meaningful intransitive gestures) but a "reverse" transitivity effect in patients with lesions of the right hemisphere (i.e., meaningful transitive gestures easier than meaningful intransitive gestures); (3) there is a strong association between meaningful intransitive and transitive (but not meaningless) gestures; (4) isolated deficits of meaningful intransitive gestures are more frequent in cases with right than left hemisphere lesions; (5) these deficits may occur in the absence of language and semantic memory impairments; (6) meaningful intransitive gesture performance seems to vary according to the emotional content of gestures (i.e., body-centered gestures and emotional valence-intensity). These findings are partially consistent with the social cognition hypothesis. Methodological recommendations are given for future studies.

Cortical Correlates of Visuospatial Switching Processes Between Egocentric and Allocentric Frames of Reference: A fNIRS Study.

Human beings represent spatial information according to egocentric (body-to-object) and allocentric (object-to-object) frames of reference. In everyday life, we constantly switch from one frame of reference to another in order to react effectively to the specific needs of the environment and task demands. However, to the best of our knowledge, no study to date has investigated the cortical activity of switching and non-switching processes between egocentric and allocentric spatial encodings. To this aim, a custom-designed visuo-spatial memory task was administered and the cortical activities underlying switching vs non-switching spatial processes were investigated. Changes in concentrations of oxygenated and deoxygenated haemoglobin were measured using functional near-infrared spectroscopy (fNIRS). Participants were asked to memorize triads of geometric objects and then make two consecutive judgments about the same triad. In the non-switching condition, both spatial judgments considered the same frame of reference: only egocentric or only allocentric. In the switching condition, if the first judgment was egocentric, the second one was allocentric (or vice versa). The results showed a generalized activation of the frontal regions during the switching compared to the non-switching condition. Additionally, increased cortical activity was found in the temporo-parietal junction during the switching condition compared to the non-switching condition. Overall, these results illustrate the cortical activity underlying the processing of switching between body position and environmental stimuli, showing an important role of the temporo-parietal junction and frontal regions in the preparation and switching between egocentric and allocentric reference frames.

Spatial representations of objects used away and towards the body: The effect of near and far space.

An action with an object can be accomplished only if we encode the position of the object with respect to our body (i.e., egocentrically) and/or to another element in the environment (i.e., allocentrically). However, some actions with the objects are directed towards our body, such as brushing our teeth, and others away from the body, such as writing. Objects can be near the body, that is within arm reaching, or far from the body, that is outside arm reaching. The aim of this study was to verify if the direction of use of the objects influences the way we represent their position in both near and far space. Objects typically used towards (TB) or away from the body (AB) were presented in near or far space and participants had to judge whether an object was closer to them (i.e., egocentric judgement) or closer to another object (i.e., allocentric judgement). Results showed that egocentric judgements on TB objects were more accurate in near than in far space. Moreover, allocentric judgements on AB objects were less accurate than egocentric judgements in near space but not in far space. These results are discussed with respect to the different roles that visuo-motor and visuo-spatial mechanisms play in near space and far space, respectively.

Paying attention to the outcome of others' actions has dissociated effects on observer's peripersonal space representation and exploitation.

The representation of peripersonal space (PPS representation) and the selection of motor actions within it (PPS exploitation) are influenced by action outcomes and reward prospects. The present study tested whether observing the outcome of others' actions altered the observer's PPS representation and exploitation. Participants (observers) performed a reachability-judgement task (assessing PPS representation) before and after having observed a confederate (actors) performing a stimuli-selection task on a touch-screen table. In the stimuli-selection task, the stimuli selected could either yield a reward or not, but the probability to select a reward-yielding stimulus was biased in space, being either 50%, 25% or 75% in the actor's proximal or distal space. After the observation phase, participants performed the stimuli-selection task (assessing PPS exploitation), but with no spatial bias in the distribution of reward-yielding stimuli. Results revealed an effect of actors' actions outcome on observers' PPS representation, which changed according to the distribution of reward-yielding stimuli in the actors' proximal and distal spaces. No significant effect of actors' actions outcome was found on observers' PPS exploitation. As a whole, the results suggest dissociated effects of observing the outcome of others' actions on PPS representation and exploitation.

The 'healthy side' of anosognosia for hemiplegia: Increased sense of agency for the unimpaired limb or motor compensation?

OBJECTIVES: Anosognosic patients show a lack of awareness for their hemiplegia coupled with a distorted sense of agency for the actions performed by the plegic limbs. Since anosognosia is often associated with right brain damage, this hemisphere seems to play a dominant role in monitoring awareness for motor actions. Therefore, we would expect that anosognosic patients show distorted awareness and sense of agency also for actions performed with the unimpaired limb. METHOD: To test this hypothesis, we induced illusory actions that could be congruent or incongruent with a preceding verbal command. A group of 16 right brain-damaged patients performed this task and then rated i) their ability to anticipate the actions, ii) their sense of agency and iii) their sense of ownership for each limb. Measures of awareness, neglect and motor impairment were also considered for the patient group. RESULTS: Following incongruent actions with the unimpaired limb, less aware patients showed a relatively mild distortion in all three aspects. In addition, we also found a crucial relationship between motor impairment (for the plegic limb) and sense of agency for both plegic and healthy limbs. CONCLUSION: Although the distortion linked to both limbs supports the initial hypothesis that the right hemisphere is responsible for monitoring awareness for action for the whole body, our data also suggest that the observed distortion may be linked to a motor compensatory phenomenon, not necessarily related to awareness processes.

Representational Processes of Actions Toward and Away from the Body.

The aim of the present study was to investigate the role of mental representation processes during the planning, reaching, and use phases of actions with tools commonly used toward the body (TB, e.g., toothbrush) or away from the body (AB, e.g., pencil). In the first session, healthy participants were asked to perform TB (i.e., making circular movements with the toothbrush near the mouth) and AB (i.e., making circular movements with the pencil near the desk) actions both with (i.e., actual use) and without the tool in hand (i.e., the pantomime of tool use). In the second session, the same participants performed a series of mental rotation tasks involving body- (i.e., face and hands) and object-related (i.e., abstract lines) stimuli. The temporal and kinematic analysis of the motor actions showed that the time required to start the pantomimes (i.e., the planning phase) was shorter for the AB action than for the TB action. In contrast, the reaching phase lasted longer for the AB action than for the TB action. Furthermore, the TB pantomime was associated with the performance in the mental rotation of body-related stimuli, especially during the planning and reaching phases, whereas the AB pantomime was more related to the performance in the mental rotation of object-related stimuli, especially during the tool use phase. Thus, the results revealed that the direction of a goal-directed motor action influences the dynamics of the different phases of the motor action and can determine the type of mental images involved in the planning and execution of the action.

Uncover the Offensive Side of Disparagement Humor: An fMRI Study.

Disparagement humor is a kind of humor that denigrates, belittles an individual or a social group. In the aim to unveil the offensive side of these kinds of jokes, we have run an event-related fMRI study asking 30 healthy volunteers to judge the level of fun of a series of verbal stimuli that ended with a sentence that was socially inappropriate but funny (disparagement joke -DJ), socially inappropriate but not funny (SI) or neutral (N). Behavioral results showed disparagement jokes are perceived as funny and at the same time offensive. However, the level of offense in DJ is lower than that registered in SI stimuli. Functional data showed that DJ activated the insula, the SMA, the precuneus, the ACC, the dorsal striatum (the caudate nucleus), and the thalamus. These activations suggest that in DJ a feeling of mirth (and/or a desire to laugh) derived from the joke (e.g., SMA and precuneus) and the perception of the jokes' social inappropriateness (e.g., ACC and insula) coexist. Furthermore, DJ and SI share a common network related to mentalizing and to the processing of negative feelings, namely the medial prefrontal cortex, the putamen and the right thalamus.

Semantic and action tool knowledge in the brain: Identifying common and distinct networks.

Most cognitive models of apraxia assume that impaired tool use results from a deficit occurring at the conceptual level, which contains dedicated information about tool use, namely, semantic and action tool knowledge. Semantic tool knowledge contains information about the prototypical use of familiar tools, such as function (e.g., a hammer and a mallet share the same purpose) and associative relations (e.g., a hammer goes with a nail). Action tool knowledge contains information about how to manipulate tools, such as hand posture and kinematics. The present review aimed to better understand the neural correlates of action and semantic tool knowledge, by focusing on activation, stimulation and patients' studies (left brain-damaged patients). We found that action and semantic tool knowledge rely upon a large brain network including temporal and parietal regions. Yet, while action tool knowledge calls into play the intraparietal sulcus, function relations mostly involve the anterior and posterior temporal lobe. Associative relations engaged the angular and the posterior middle temporal gyrus. Moreover, we found that hand posture and kinematics both tapped into the inferior parietal lobe and the lateral occipital temporal cortex, but no region specificity was found for one or the other representation. Our results point out the major role of both posterior middle temporal gyrus and inferior parietal lobe for action and semantic tool knowledge. They highlight the common and distinct brain networks involved in action and semantic tool networks and spur future directions on this topic.

Identifying hotspots of non-indigenous species' high impact in the Maltese islands (Central Mediterranean Sea).

Several invasive non-indigenous species (NIS) cause ecological and socio-economic impacts. A good understanding of the impacts of invasive NIS in Mediterranean habitats is important for managing and prioritising measures in the marine environment. We applied a conservative additive model to sum up the Cumulative IMPacts of invasive Alien (CIMPAL) species of 19 invasive marine NIS on 13 habitats in Maltese waters. This analysis identified three hotspot areas of NIS' high impact and five top-priority together with four high-priority invasive NIS for management. By using CIMPAL, it was also possible to differentiate between areas within the same Marine Protected Area. CIMPAL is thus recommended as a good tool for managers and policy makers for prioritising measures as well as funds. Finally, recommendations are made with respect to future steps that are needed for the CIMPAL applicability, aiming to a more appropriate decision-making on prioritisation of hotspot areas and invasive marine NIS.

Towards and away from the body: The relevance of the direction of use in the coding of object-related actions.

This study examines whether the perception of an object automatically activates the representation of the direction of use of that object. To this aim, we carried out two experiments. In Experiment 1, participants were asked to explicitly categorise objects as used either away from the body (AB, for example, a hammer) or towards the body (TB, for example, a toothbrush). In Experiment 2, participants were asked to judge whether the same objects were natural or manmade. In both experiments, they were asked to respond by moving a joystick backwards (i.e., TB) or frontwards (i.e., AB). Therefore, their response could either be congruent (i.e., backward response with TB objects, frontward response with AB objects) or incongruent (i.e., backward response with AB objects, frontward response with TB objects) with the direction of object use. Results from Experiment 1 showed that in the congruent condition, participants were faster in judging the direction of object use than those in the incongruent condition (congruency effect). Crucially, results from Experiment 2 showed the presence of a congruency effect even when the direction of object use was task-irrelevant. However, this effect was found only for TB objects. These results suggest that the perception of TB objects automatically activates the direction of object use with respect to the body, thus showing evidence of direct connection between perception and action. A specific role of the body might account for different action representation processes involved in TB and AB object-related actions.

The role of mental imagery in pantomimes of actions towards and away from the body.

This study aimed to explore the relationship between action execution and mental rotation modalities. To this end, pantomime gesture (i.e. the mime of the use of an object) was used as its execution relies on imagery processes. Specifically, we tried to clarify the role of visuo-spatial or motor and body-related mental imagery processes in pantomime gestures performed away (AB, e.g. drawing on a sheet) and towards the body (TB, e.g. brushing the teeth). We included an "actual use" condition in which participants were asked to use a toothbrush and make 3, 6, or 9 circular movements close to their mouth (as if they were brushing their teeth) or to use a pencil and make 3, 6, or 9 circular movements on a desk (as if they were drawing circles). Afterwards, participants were asked to pantomime the actual use of the same objects ("pantomime" condition). Finally, they were asked to mentally rotate three different stimuli: hands, faces, and abstract lines. Results showed that participants were faster in AB than TB pantomimes. Moreover, the more accurate and faster the mental rotation of body-related stimuli was, the more similar the temporal duration between both kinds of pantomimes and the actual use of the objects appeared. Instead, the temporal similarity between AB pantomimes and pencil actual use, as well as, the duration of AB pantomime and actual use, were associated with the ability to mentally rotate abstract lines. This was not true for TB movements. These results suggest that the execution of AB and TB pantomimes may involve different mental imagery modalities. Specifically, AB pantomimes would not only require to mentally manipulate images of body-parts in movement but also represent the spatial relations of the object with the external world.

Affective, Social, and Informative Gestures Reproduction in Human Interaction: Hyperscanning and Brain Connectivity.

Gestural communication characterizes daily individuals' interactions in order to share information and to modify others' behavior. Social neuroscience has investigated the neural bases which support recognizing of different gestures. The present research, through the use of the hyperscanning approach, that allows the simultaneously recording of the activity of two or more individuals involved in a joint action, aims to investigate the neural bases of gestural communication. Moreover, by using hyperscanning paradigm we explore the inter-brain connectivity between two inter-agents, the one who performed the gesture (encoder) and the one who received it (decoder), with functional Near-infrared Spectroscopy (fNIRS) during the reproduction of affective, social and informative gestures with positive and negative valence. Result showed an increase in oxygenated hemoglobin concentration (O2Hb) and inter-brain connectivity in the dorsolateral prefrontal cortex (DLPFC) for affective gestures, in the superior frontal gyrus (SFG) for social gestures and the frontal eye fields (FEF) for informative gestures, for both encoder and decoder. Furthermore, it emerged that positive gestures activate more the left DLPFC, with an increase in inter-brain connectivity in DLPFC and SFG. The present study revealed the relevant function of the type and valence of gestures in affecting intra- and inter-brain connectivity.

Pantomime of tool use: looking beyond apraxia.

Pantomime has a long tradition in clinical neuropsychology of apraxia. It has been much more used by researchers and clinicians to assess tool-use disorders than real tool use. Nevertheless, it remains incompletely understood and has given rise to controversies, such as the involvement of the left inferior parietal lobe or the nature of the underlying cognitive processes. The present article offers a comprehensive framework, with the aim of specifying the neural and cognitive bases of pantomime. To do so, we conducted a series of meta-analyses of brain-lesion, neuroimaging and behavioural studies about pantomime and other related tasks (i.e. real tool use, imitation of meaningless postures and semantic knowledge). The first key finding is that the area PF (Area PF complex) within the left inferior parietal lobe is crucially involved in both pantomime and real tool use as well as in the kinematics component of pantomime. The second key finding is the absence of a well-defined neural substrate for the posture component of pantomime (both grip errors and body-part-as-tool responses). The third key finding is the role played by the intraparietal sulcus in both pantomime and imitation of meaningless postures. The fourth key finding is that the left angular gyrus seems to be critical in the production of motor actions directed towards the body. The fifth key finding is that performance on pantomime is strongly correlated with the severity of semantic deficits. Taken together, these findings invite us to offer a neurocognitive model of pantomime, which provides an integrated alternative to the two hypotheses that dominate the field: The gesture-engram hypothesis and the communicative hypothesis. More specifically, this model assumes that technical reasoning (notably the left area PF), the motor-control system (notably the intraparietal sulcus), body structural description (notably the left angular gyrus), semantic knowledge (notably the polar temporal lobes) and potentially theory of mind (notably the middle prefrontal cortex) work in concert to produce pantomime. The original features of this model open new avenues for understanding the neurocognitive bases of pantomime, emphasizing that pantomime is a communicative task that nevertheless originates in specific tool-use (not motor-related) cognitive processes. .

The combined effects of motor and social goals on the kinematics of object-directed motor action.

Voluntary actions towards manipulable objects are usually performed with a particular motor goal (i.e., a task-specific object-target-effector interaction) and in a particular social context (i.e., who would benefit from these actions), but the mutual influence of these two constraints has not yet been properly studied. For this purpose, we asked participants to grasp an object and place it on either a small or large target in relation to Fitts' law (motor goal). This first action prepared them for a second grasp-to-place action which was performed under temporal constraints, either by the participants themselves or by a confederate (social goal). Kinematic analysis of the first preparatory grasp-to-place action showed that, while deceleration time was impacted by the motor goal, peak velocity was influenced by the social goal. Movement duration and trajectory height were modulated by both goals, the effect of the social goal being attenuated by the effect of the motor goal. Overall, these results suggest that both motor and social constraints influence the characteristics of object-oriented actions, with effects that combine in a hierarchical way.

Effect of test instructions: The example of the pantomime production task.

The production of pantomime is a sensible task to detect praxis deficits. It is usually assessed by presenting objects visually or by verbal command. Verbal instructions are given either by providing the name of the object (e.g., "Show me how to use a pen") or by requiring the object function (e.g., "Show me how to write"). These modes of testing are used interchangeably. The aim of this study is to investigate whether the different instructions generate different performances. Fifty-one healthy participants (17-89 years old) were assessed on three pantomime production tasks differing for the instruction given: two with verbal instructions (Pantomime by Name and Pantomime by Function) and one with the object visually presented (Pantomime by Object). Results showed that Pantomime by Function produced the poorest performance and the highest frequency of Body Parts as Tool (BPT) errors, suggesting that the way the instructions are given may determine the performance in a task. Nuances in test instructions could result in misleading outcome.

Activation of manipulation and function knowledge during visual search for objects.

This study aimed at comparing the time course of the activation of function and manipulation knowledge during object identification. The influence of visual similarity and context information was also assessed. In 3 eye-tracking experiments, conducted with the Visual-World-Paradigm, participants heard the name of an object and had to identify it among four pictures. The target object (e.g., shopping cart) could be presented along with objects related by (a) function (e.g., basket), (b) manipulation (e.g., lawnmower), (c) context (e.g., cash register), (d) visual similarity (e.g., toaster), and (e) completely unrelated objects. Growth curve analyses were used to assess competition effects among semantically (a, b, and c), visually related (d), and unrelated competitors (e). Results showed that manipulation- and function-related, but not context-related objects received more fixations than the unrelated ones, with a temporal advantage for the manipulation-related objects (Experiment 1). However, the visually similar objects faded the semantic competition effects, especially for function-related objects (Experiment 2). Finally, no temporal differences appeared when manipulation- and function-related objects were shown within the same visual array (Experiment 3). These results support the idea that both function and manipulation are relevant features of object semantic representations, but in the absence of other semantic competitors the activation of manipulation features appears prioritized during object identification. (PsycINFO Database Record (c) 2019 APA, all rights reserved).