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1.
Anal Chem ; 73(19): 4558-65, 2001 Oct 01.
Article in English | MEDLINE | ID: mdl-11605831

ABSTRACT

This paper describes the adsorption mechanisms and aggregation properties of cetyltrimethylammonium bromide (CTAB) and didodecyldimethylammonium bromide (DDAB) surfactants that are used for dynamic coatings in capillary electrophoresis (CE). Atomic force microscopy is used to directly visualize surfactant adsorption on fused silica. It was found that the single-chained surfactant CTAB forms spherical aggregates on silica while the double-chained surfactant DDAB forms a bilayer. Aggregation at the surface occurs at approximately the same surfactant concentration in which EOF reversal is observed in CE. The nearest-neighbor distance between CTAB aggregates varies inversely with buffer pH and becomes constant at the point when the silanol groups are fully ionized. DDAB forms a flat, uniform coating independent of pH. Increasing the buffer ionic strength changes the morphology of the CTAB aggregates from spherical to cylindrical. The change in morphology can alter the surface coverage, which is related to the "normalized" EOF measured in identical buffers. The morphology of a surfactant coating is also shown to affect its ability to inhibit protein adsorption to the capillary wall. Specifically, the full surface coverage provided by DDAB proved superior in a head-to-head comparison with CTAB.

2.
Electrophoresis ; 22(1): 52-8, 2001 Jan.
Article in English | MEDLINE | ID: mdl-11197179

ABSTRACT

In capillary electrophoresis, the short optical path length associated with on-column UV detection imposes an inherent detection problem. Detection limits can be improved using sample stacking. Recently, large-volume sample stacking (LVSS) without polarity switching was demonstrated to improve detection limits of charged analytes by more than 100-fold. However, this technique requires suppression of the electroosmotic flow (EOF) during the run. This necessitates working at a low pH, which limits using pH to optimize selectivity. We demonstrate that LVSS can be performed at any buffer pH (4.0-10.0) if the zwitterionic surfactant Rewoteric AM CAS U is used to suppress the EOF. Sensitivity enhancements of up to 85-fold are achieved with migration time, corrected area, and peak height reproducibility of 0.8-1.6%, 1.3-3.7%, and 0.8-4.9%, respectively. Further, it is possible to stack either positively or negatively charged analytes using zwitterionic surfactants to suppress the EOF.


Subject(s)
Electrophoresis, Capillary/methods , 3,4-Dihydroxyphenylacetic Acid/isolation & purification , Betaine/analogs & derivatives , Betaine/chemistry , Brompheniramine/isolation & purification , Catecholamines/isolation & purification , Cations , Chlorpheniramine/isolation & purification , Dihydroxyphenylalanine/isolation & purification , Diphenhydramine/isolation & purification , Doxylamine/isolation & purification , Histamine H1 Antagonists/isolation & purification , Hydrogen-Ion Concentration , Pheniramine/isolation & purification , Surface-Active Agents/chemistry
3.
Anal Chem ; 72(10): 2280-4, 2000 May 15.
Article in English | MEDLINE | ID: mdl-10845375

ABSTRACT

The zwitterionic surfactant Rewoteric AM CAS U forms a dynamic wall coating that prevents the adsorption of cationic proteins as well as suppresses the electroosmotic flow (EOF). Addition of polarizable anions to buffers containing this zwitterionic surfactant increases the once suppressed EOF to values nearing +3 x 10(-4) cm2/(V s). The retention of the EOF allows for the separation of analytes of widely different mobilities and is demonstrated by the simultaneous separation of cationic and anionic proteins. Using a buffer containing optimal amounts of the polarizable anion perchlorate and surfactant CAS U, the proteins lysozyme, ribonuclease A, alpha-chymotrypsinogen A, and myoglobin are separated in less than 15 min. Efficiencies as high as 1.5 million plates/m and recoveries greater than 91% are observed for proteins injected in distilled water. Migration time reproducibility is approximately 1% RSD within 1 day and approximately 3% RSD from day to day. The anionic and cationic proteins can be separated over a pH range of 5.5-9, all yielding good efficiencies.


Subject(s)
Betaine/analogs & derivatives , Proteins/isolation & purification , Animals , Anions , Cations , Cattle , Chymotrypsinogen/isolation & purification , Electrophoresis, Capillary/methods , Muramidase/isolation & purification , Myoglobin/isolation & purification , Ribonuclease, Pancreatic/isolation & purification
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