ABSTRACT
Using scanning electron microscopy and semiautomatic image analyser, regularities and quantitative changes were studied in endotheliocytes in three regions of thoracic aorta during ageing of normal and spontaneously hypertensive rats of the Kyoto-Wistar line. The data of morphometric analysis and the results of simulating endothelial cell monolayer changes, by means of construction of polyrow mosaics, suggest that the number of small endotheliocytes of normotensive rats in all aorta regions is lowered in the period from 2 to 10 months to increase then from 10 to 16 months. The endothelial cell monolayer changes occur as follows: the small endotheliocytes appear and then disappear, keeping their random distribution in the endothelial layer. Within the same period, in spontaneously hypertensive rats, the behavior of the endothelial layer in the region of intercostal arteria ostia and on the dorsal surface is similar to that in normal rats, being presumably associated with age changes. In hypertensive rats, on the ventral surface of the aorta, a relative number of small cells increases at the expense of small cell foci formation. Thus, the provided evidence points to a heterogeneity of responses of endothelial layers in different regions of the aorta on the constant elevation of pressure.
Subject(s)
Aorta, Thoracic/ultrastructure , Endothelium, Vascular/ultrastructure , Rats, Inbred SHR/anatomy & histology , Aging/pathology , Animals , Hypertension/pathology , Male , Microscopy, Electron, Scanning/methods , Rats , Rats, Inbred WKY , TelevisionABSTRACT
A new specific cholesterol marker and scanning electron microscopy were used to study the quantity and distribution of cholesterol in the artifical phospholipid membranes and in the plasmalemma of animal and human endotheliocyte and erythrocytes in health and in hypercholesterolemia.
Subject(s)
Cholesterol/analysis , Endothelium, Vascular/ultrastructure , Erythrocyte Membrane/ultrastructure , Membranes, Artificial , Animals , Aorta/chemistry , Aorta/ultrastructure , Arteriosclerosis/blood , Cats , Cell Membrane/chemistry , Cell Membrane/ultrastructure , Endothelium, Vascular/chemistry , Erythrocyte Membrane/chemistry , Guinea Pigs , Humans , Hypercholesterolemia/blood , Microscopy, Electron, Scanning , Microspheres , Rabbits , Rats , Species Specificity , Surface PropertiesABSTRACT
The results of quantitative analysis of regional differences in the structure of thoracic aorta endotheliocytes in normal and hypertensive rats are presented. Scanning electron microscopy and semiautomatic image analyser were employed in studying the cellular structure in three regions of aorta endothelial layer: dorsal and ventral surfaces and region of the intercostal arterial ostia. The data obtained show that in cases of a short-duration hypertension the most pronounced are the alterations in endotheliocytes of the dorsal surface. Thus, the results confirm the heterogeneity of the aortic endothelium under normal conditions and demonstrate that differences in overlapping of neighbouring endotheliocytes is the cause of the observed alterations in cases of hypertension.
Subject(s)
Aorta/pathology , Endothelium, Vascular/pathology , Hypertension/pathology , Animals , Aorta/ultrastructure , Endothelium, Vascular/ultrastructure , Male , Microscopy, Electron, Scanning , RatsABSTRACT
Three different approaches for studying aorta endothelial cells by scanning electron microscopy (SEM) were compared to expose cell border lines and surface shape simultaneously. Staining of aorta probes with silver nitrate leads to more specific silver deposits at cell junctions, but unsatisfactory image resolution obscures any detail of cell junctions and other fine structural features. Endothelium impregnated with tannin-osmium showed enhanced cell junction detail, but this method resulted in formation of presumably artificial structures. Osmium tetraoxide was most optimal for impregnation of aorta probes, as it exposed well cell border lines, excluded artifacts of tannin-osmium staining, provided a good image resolution in SEM, and allowed to analyse the ultrastructural features of cell surface.
Subject(s)
Endothelium, Vascular/ultrastructure , Animals , Aorta, Abdominal/ultrastructure , Male , Microscopy, Electron, Scanning/methods , Rats , Staining and Labeling/methods , Surface PropertiesABSTRACT
Three-dimensional organization of the liver vascular bed in (C57B1/6 X CBA)F1 mice and rats was examined by scanning electron microscopy of injection replicas. The data have been obtained on the character of branching, diameter of injection replicas in arterial and venous vasculature and different types of microvascular surface relief, depending on their location in the vascular bed.
Subject(s)
Liver/blood supply , Microscopy, Electron, Scanning , Animals , Capillaries/anatomy & histology , Female , Hepatic Artery/anatomy & histology , Male , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Microscopy, Electron, Scanning/methods , Portal Vein/anatomy & histology , RatsABSTRACT
By means of SEM-analysis in 110 microphotos (from 14 aortas of white rats) with the aim to estimate heteromorphism of the thoracic part endothelium, quantitative characteristics of endotheliocytes have been studied in the ventral and dorsal surfaces, and also around the ostia of the intercostal arteries. For the quantitative analysis organization of the lines of the interendotheliocyte borders is taken into account. Endotheliocytes around the ostium are more elongated but occupy less area and have less straight contours in comparison with cells in other regions; they have also greater variability. Essential differences in arranging the endothelial cells into the layer on the ventral and dorsal surfaces of the aorta are revealed.
