[Effect of potassium leakage on reversible aggregation of human erythrocytes]. / Vliianie utechki kaliia na obratimuiu agregatsiiu éritrotsitov cheloveka.

Changes in the aggregation of human erythrocytes caused by polydextrane were studied under conditions influencing the rate of potassium leakage from cells to medium. It was shown that aggregation decreases as the leakage of potassium ions increases and is completely abolished at leakage rates higher than 2.5-3.0 mmol/l of erythrocytes per hour. The involvement of nonequilibrial electrokinetic phenomena in the inhibition of erythrocyte aggregation by ionic fluxes across erythrocyte surface is discussed. It is proposed that potassium leakage affects the erythrocyte sedimentation rate in clinical investigations.

[The effect of nonequibrilium states of membrane-area electrolyte during dilution of cell suspensions]. / Vliianie neravnovesnykh sostoianii primembrannogo sloia élektrolita na techenie razbavlennykh kletochnykh suspenzii.

The flow rate distribution in the diluted suspension of human red blood cells was measured across the long rectangular capillary. The calculated rate distribution of Pousaseil's flow was shown to be spoiled, if the substances influencing the red blood cell aggregation were added to the suspension. The Pousaseil's flow rate distribution takes place under the same conditions which support the sedimentation rate distribution predicted for the small particles without interaction. The sedimentation and flow anomalies are suggested to arise from the simultaneous lateral media flow along the cell surface and ion transfer across the cell membrane.

[The effect of cholesterol level in erythrocyte membranes on the sedimentation rate, electrophoretic motility, and the rate of potassium ferricyanide reduction]. / Vliianie soderzhaniia kholesterina v membranakh éritrotsitov na skorost' osedaniia, elektroforeticheskuiu podvizhnost' i skorost' vosstanovleniia ferritsianida kaliia.

It has been found that with an increase of the molar ratio cholesterol/phospholipids in human erythrocyte membranes in vitro their sedimentation rate in diluted suspensions increases and the reduction rate of potassium ferricyanide decreases, while the electrophoretic mobility of erythrocytes is not changed. The effect of cholesterols discussed in terms of the mechanism of transmembrane transfer of reduction equivalents and non-equilibrium in the double electric layer of erythrocytes surface.

[Erythrocyte sedimentation rate in diluted suspensions and their electrophoretic mobility in a vertical electrical field]. / Skorost' osedaniia éritrotsitov v razbavlennykh suspenziiakh i ikh élektroforeticheskaia podvizhnost' v vertikal'nom élektricheskom pol pole.

The sedimentation rates (SR) of human red blood cells (RBC) were measured in diluted suspensions using the thin plate chamber. If the suspension medium was phosphate buffer saline or 0,18 M NaCl the SR-dependence on the distance to the chambers wall corresponded to SR distribution of small particles without interaction. The more NaCl content was decreased down to 0.145 M, the more temperature-dependent variations of SR were noted, while SR distribution became distinct with the predicted one for the non-interacting particles. The use of SR distribution is discussed for testing the RBC interaction in diluted suspensions caused by sedimentation. The electrophoretic measurements carried out under vertical oriented electrical field showed the rate of RBC movement to be the linear function of the field gradient and to be not influenced under the SR modifying conditions.

[Effect of nitrofuran derivatives on the oxygen consumption by rat liver mitochondria]. / Vliianie nekotorykh nitrofuranovykh proizvodnykh na potreblenie kisloroda mitokhondriiami pecheni krys.

Utilization of oxygen in rat liver mitochondria was studied using two nitrofuran derivatives 5-nitro-2-furaldehyde semicarbazone (furacin) and soluble derivative of nitrofuran--M-106-P. The substances at 10(-3) M concentration increased O2 consumption in mitochondria with simultaneous decrease in the respiratory control. After blocking of respiration with KCN, antimycine, rhothenone, the nitrofurans stimulated O2 consumption in mitochondria up to 30% as compared with controls.