Effects of different wavelengths of low-level laser therapy on orthodontically induced inflammatory root resorption in rats investigated with micro-computerized tomography.

INTRODUCTION: The objective of this research was to investigate the effects of different wavelengths low-level laser therapies on orthodontically induced inflammatory root resorption (OIIRR) during orthodontic tooth movement in rats by micro-computerized tomography. METHODS: Forty Wistar albino rats were divided into 5 groups: control group (A), 405-nm laser group (B), 532-nm laser group (C), 650-nm laser group (D), and 940-nm laser group (E). The left side of group A was used as a positive control (A-PC), and the right side of group A was used as a negative control (A-NC) group. In all groups, the maxillary left first molars were moved mesially by 50 g of force for 14 days. The lasers were performed for 9 minutes on the maxillary left first molar tooth. At the end of the experimental period, OIIRR measurements were performed at the mesial and the distal sides along the mesial root of the maxillary first molars. RESULTS: The root resorption volume was significantly lower in group A-NC than in groups A-PC, B, and D. The percentage of root resorption was significantly lower in group A-NC than in all other groups. The root resorption volume and the percentage of root resorption in groups C, D, and E were significantly lower than group A-PC. The depth and the width of the lacuna and even the number of mesial lacunae were similar between groups. The distal and the total lacunae were significantly lower in group A-NC than in all other groups except group C. CONCLUSIONS: The 532-nm, 650-nm, and 940-nm lasers significantly reduced the volume of OIIRR. In addition, the 532-nm laser reduced the number of lacunae both distally and totally than all the other groups.

Effects of 405-, 532-, 650-, and 940-nm wavelengths of low-level laser therapies on orthodontic tooth movement in rats.

BACKGROUND: Investigating the effects of 405-nm, 532-nm, 650-nm, and 950-nm wavelengths of LLLTs (low-level laser therapies) on the orthodontic tooth movement in rats by using histological and immunohistochemical methods. Forty-five Wistar albino rats were randomly divided into 5 groups: control group (positive control: the left maxillary 1st molar side; negative control: the right maxillary 1st molar side), 405 nm LLLT group (Realpoo), 532 nm LLLT group (Realpoo), 650 nm LLLT group (Realpoo), and 940 nm LLLT group (Biolase). The left maxillary 1st molar teeth of all rats were applied mesially 50-g force. Starting from the 1st day, 48 h intervals, LLLT was applied in continuous wave mode and in contact with the tissue. The application area was approximately 1 cm2. The lasers were performed for 3 min on each surface (buccal, palatal, mesial), totally 9 min (total dose 54 J/cm2). The amount of the molar mesialization, the bone area between the roots, PDL (periodontal ligament) measurements, TRAP (tartrate-resistant acid phosphatase), and ALP (alkaline phosphatase) immunoreactivity intensity were calculated. RESULTS: The amount of the molar mesialization was significantly higher in the 650 nm LLLT group (mean 0.878 ± 0.201 mm; 95% CI (confidence interval) 0.724 and 1.032) than in the groups of positive control (mean 0.467 ± 0.357 mm; 95% CI 0.192 and 0.741) and 405 nm LLLT (mean 0.644 ± 0.261 mm; 95% CI 0.443 and 0.845) (p < 0.001). There were significant differences in the PDL-mesial (p = 0.042) and PDL-distal (p = 0.007) regions between the groups. The immunoreactivity intensity for TRAP-mesial was significantly higher in the positive control group (mean 109,420.33 ± 8769.17; 95% CI 100,217.65 and 118,623.02) than in the 405 nm (mean 91,678.83 ± 7313.39; 95% CI 84,003.9 and 99,353.77) and the 650 nm LLLT (mean 87,169.17 ± 4934.65; 95% CI 81,990.56 and 92,347.77) groups (p = 0.002). There was no statistically significant difference between the groups on immunoreactivity intensity with ALP staining. CONCLUSIONS: The results of this study show that LLLT with 650-nm wavelength increases orthodontic tooth movement more than 405-nm, 532-nm, and 940-nm LLLTs. The 940-nm and 650-nm LLLTs also increase the bone area between the roots by more than 405-nm and 532-nm wavelengths.

Effects of Different Head Positioning Methods on Facial Soft Tissue Analysis Using Stereophotogrammetry.

PURPOSE: The purpose of this study was to compare the sagittal tilt of the head in different head positioning techniques using an inclinometer and facial stereophotogrammetric measurements. MATERIALS AND METHODS: The study was carried out in 45 participants (26 female, 19 male). Participants' head positioning was obtained with dynamic walking, Frankfort horizontal plane (FHP'), self-balance plus mirror, and subjective photographic positioning methods. All pitch values were recorded by an inclinometer and stereophotogrammetric images were obtained. Facial analysis included distances of the glabella (G'), pronasale (Pn), soft tissue A point (A'), upper lip (Ls), lower lip (Li), soft tissue B point (B)', and soft tissue pogonion (Pog') to the true vertical line (TVL) and face height and lip length measurements. RESULTS: Participants' head positions were observed to be more forward in the FHP' head positioning technique compared with other methods, whereas a more backward head position was recorded with subjective head positioning, and the difference was significant (P < .001). There were no relevant differences in pitch values between the self-balance plus mirror and dynamic walking methods. G'-TVL (P < .000), Pn-TVL (P < .029), A'-TVL (P < .039), Ls-TVL (P < .001), Li-TVL (P < .037), B'-TVL (P < .003), and Pog'-TVL (P < .000) in the profile view and face height, lower face height, and lower lip length values in the frontal view (P < .001) differed significantly by head positioning method. CONCLUSIONS: The dynamic walking and self-balance plus mirror head positioning methods offered similar and advisable natural head position results, whereas FHP' head positioning was questionable for an accurate determination of natural head position. Facial soft tissue measurements, such as face height, lower face height, lower lip length, and projection of structures such as the G', Pn, lips, and chin, varied based on head positioning method.