Meal timing across the day modulates daily energy intake in adult patients with type 2 diabetes.

BACKGROUND/OBJECTIVES: We assessed the association between the timing of meals across the day with diet composition and metabolic parameters in patients with type-2 diabetes (T2D). SUBJECTS/METHODS: Eighty adults (55.2 ± 6.8 years, 45% males) patients with T2D (without insulin therapy) were included. Three non-consecutive dietary records assessed food intake. The onset time of each consumed meal/beverage was identified and assigned to one of three periods of the day: Period 1 (P1, 06:00-11:59 h), Period 2 (P2, 12:00-17:59 h), and Period 3 (P3, 18:00-00:30 h). RESULTS: Energy intake in P1 was lower compared to P2 and P3 (22.8 ± 7.9%, 37.5 ± 9.6%, and 39.7 ± 9.9%, respectively, P < 0.001). The same pattern was found for both total protein and fat intake, but carbohydrate intake was similar among periods. Patients with greater daily energy intake (as % of total energy) in P3 showed increased total food consumption, total energy, protein, and fat intake (all P < 0.05). The opposite pattern was observed in patients with greater daily energy intake in P1 (all P < 0.05). Regression analysis showed that daily energy intake was significantly reduced when a higher proportion of carbohydrates was eaten in P1 (vs. P3, P < 0.04). CONCLUSION: Increased energy intake late during the day is related to increased total food and daily energy intake in patients with T2D. A greater proportion of total carbohydrates eaten early during the day relates to lower total energy intake. Our results suggest that earlier food intake may be a nutritional tool for dietary and metabolic control in these patients.

Contribución de las organizaciones sociales "ollas comunes" a la alimentación de la población chilena en tiempos de pandemia por COVID-19

RESUMEN Las "ollas comunes" (OC) son organizaciones sociales temporales que entregan alimentación gratuita en periodos de crisis. En Chile, resurgen el año 2020 a raíz de la pandemia del COVID-19, y si bien han aparecido en otros períodos de la historia, no ha sido cuantificada su contribución. El objetivo de este estudio fue determinar su contribución a la alimentación y nutrición de las/os chilenas/os, mediante la caracterización y análisis de su oferta alimentaria. Se realizó un estudio descriptivo mediante la aplicación de una encuesta online a voluntarias/os de todo el territorio nacional, la cual incluyó una caracterización del encuestado/a, de la OC que representa y de las preparaciones ofrecidas, en base a lo cual se obtuvo el aporte calórico y nutricional. Contestaron 117 OC (5,1% zona norte, 85,5% centro y 9,4% sur) cuya contribución principal fue con almuerzos (95,9%) con una mediana de 150 (p25: 100 - p75: 200) raciones diarias, compuestas por plato de fondo (95,9%), pan (80,5%) y ensalada (74,8%). Las preparaciones más prevalentes fueron legumbres (91,1%), arroz (78%) y fideos (77,2%). Estos almuerzos aportaron en promedio un 23% del requerimiento energético diario y aportan con el 35% del requerimiento de fibra, pero su aporte en vitamina B12, zinc y hierro fue bajo respecto del requerimiento y aporte esperado para el tiempo de comida. Hasta la fecha, estos datos son los primeros publicados sobre el tema, siendo útiles para determinar su aporte a la seguridad alimentaria del país en tiempos de crisis.

ABSTRACT "Communal Pots" (CP) are temporary social organizations that provide free food in times of crisis. In Chile, as a result of the COVID-19 pandemic, in 2020 the CP resurfaced and, although they have appeared in other periods of history, how they benefitted the population is unknown. The objective of this study is to determine the contribution of the CP to the diet and nutrition of Chileans, through the characterization and analysis of their food supply. For this, a descriptive study was carried out based on an online survey sent to volunteers from all over the country after signing the informed consent. The survey included characterization of the respondent and the CP they represented, the preparations offered by the CP, based on which the caloric and nutritional contribution was obtained. In total, 117 CP answered; 5.1% from the north, 85.5% from the center and 9.4% from the south of Chile. Their main contribution was providing lunches (95.9%) with a median of 150 (p25: 100 - p75: 200) daily rations, made up of a main entree (95.9%), bread (80.5%) and salad (74.8%). The most popular preparations were legumes (91.1%), rice (78%) and noodles (77.2%). These lunches provide an average of 23% of the daily energy requirement and 35% of the fiber requirement, but their contribution of vitamin B12 (2.5%), zinc (25%) and iron (28% req. woman) was low regarding the requirement and expected contribution for the meal time. To date, these data are the first published, to report on the real influence of this social movement in the country's food security in times of crisis.

Zinc absorption and zinc status are reduced after either sleeve gastrectomy or Roux-en-Y gastric bypass in premenopausal women with severe obesity studied prospectively over 24 postoperative months.

BACKGROUND: Roux-en-Y gastric bypass (RYGBP) is known to reduce zinc absorption; the effects of vertical sleeve gastrectomy (SG) and its long-term implications on zinc absorption have not yet been studied. OBJECTIVE: The aim was to evaluate the effects of SG and RYGBP on zinc absorption and zinc status in premenopausal women with severe obesity up to 24 mo after surgery. METHODS: Twenty-six premenopausal women undergoing SG [BMI (in kg/m2): 37.3 ± 3.2] and 32 undergoing RYGBP (BMI: 42.0 ± 4.2) were studied. A series of anthropometric, dietary, and zinc status parameters (plasma and hair zinc), and the size of the exchangeable zinc pool (EZP), as well as percentage zinc absorption from a standardized dose using a stable isotope methodology were evaluated in the patients before the surgical procedure and at 12 and 24 mo after SG or RYGBP. SG patients received 15 mg and RYGBP received 25 mg of supplemental Zn/d. RESULTS: In premenopausal women, zinc absorption was decreased by 71.9% and 52.0% in SG and RYGBP, respectively, 24 mo postsurgery, compared with initial values. According to 2-factor repeated-measures ANOVA, time effect was significant (P = <0.0001), but not time × group interaction (P = 0.470). Plasma zinc below the cutoff point of 70 µg/dL increased from 0 to 15.4% and 38.1% in SG and RYGBP, respectively. Mean EZP was significantly reduced 24 mo after surgery, although no time × group interactions were observed. Hair zinc did not change across time or between groups. CONCLUSIONS: Both SG and RYGBP have profound effects on zinc absorption capacity, which are not compensated for after 24 mo. Although zinc absorption reduction was similar in both types of surgeries, plasma zinc was more affected in RYGBP than SG, despite greater zinc supplementation in RYGBP.This trial was registered at http://www.isrctn.com as ISRCTN31937503.

La suplementación con zinc reduce la concentración de ácidos grasos libres en pacientes con diabetes tipo 2

RESUMEN Elevadas concentraciones de ácidos grasos libres (AGL) han sido asociadas con la patogénesis de resistencia a la insulina y diabetes tipo 2 (DT2), por lo que la regulación de la lipólisis resulta prioritaria en estos pacientes. El zinc mediante sus acciones insulinomiméticas e inducción de fosfodiesterasas podría regular la liberación de AGL desde el tejido adiposo. El objetivo de esta investigación fue evaluar en pacientes con DT2 el efecto de 24 meses de suplementación con zinc sobre las concentraciones séricas de AGL en ayuno. Para este propósito: se realizó la determinación de AGL en ayuno por colorimetría enzimática, zinc plasmático por espectrofotometría de absorción atómica, y parámetros bioquímicos y antropométricos de interés en 60 pacientes con DT2 que fueron asignados aleatoriamente para recibir suplementación con 30 mg/día de zinc (n= 30) o placebo (n= 30) por 24 meses. El grupo zinc presentó menor concentración sérica de AGL al mes 24 (p = 0,034). El cambio en el índice de masa corporal, el sexo y la suplementación con zinc contribuyeron significativamente como predictores de la concentración sérica de AGL al mes 24 (R= 0,493, R2= 0,243, p= 0,001). Conclusión: la suplementación con 30 mg/día de zinc en pacientes con DT2 tuvo un efecto significativo en reducir la concentración sérica de AGL en ayuno tras 24 meses de tratamiento. Estos resultados apoyan los beneficios del zinc como coadyuvante en el tratamiento de DT2.

ABSTRACT High concentrations of free fatty acids (FFA) have been associated with the pathogenesis of insulin resistance and type 2 diabetes (T2D), making lipolysis regulation a priority in these patients. Through its insulin-mimetic actions and phosphodiesterase induction, zinc could regulate FFA release from adipose tissue. The objective of this research was to evaluate the effect of 24 months of zinc supplementation on fasting serum FFA concentrations in patients with T2D. For this purpose: fasting FFA by enzymatic colorimetric, plasma zinc by atomic absorption spectrophotometry, and biochemical and anthropometric parameter of interest were determined in 60 T2D patients who were randomly assigned to 30 mg/day of zinc supplementation (n= 30) or placebo (n= 30) for 24 months. The zinc group had lower serum FFA concentration at month 24 (p= 0.034). Body mass index change, gender, and zinc supplementation contributed significantly as predictors of serum FFA concentration at month 24 (R= 0.493, R2= 0.243, p= 0.001). Conclusion: Supplementation with 30 mg/day of zinc in patients with T2D had a significant effect in reducing serum fasting FFA concentration after 24 months of treatment. These results support the benefits of zinc as coadjutant in T2D treatment.

Fatty acid desaturation in red blood cell membranes of patients with type 2 diabetes is improved by zinc supplementation.

BACKGROUND/OBJECTIVE: Membrane flexibility can be a determining factor in pathophysiological mechanisms of type 2 diabetes (T2D). As a cofactor of delta-5 desaturase (D5D) and delta-6 desaturase (D6D), and gene expression regulator, zinc may play a role modulating membrane flexibility by increasing membrane polyunsaturated fatty acids (PUFA) abundance. The objective of this study was to evaluate the effect of a 24-month zinc supplementation (30 mg elemental zinc) on membrane fatty acid composition in patients with T2D. SUBJECTS/METHODS: Sixty patients with T2D were evaluated. Thirty were randomly assigned to the zinc supplemented group and thirty to the placebo group. Fatty acid composition in red blood cell (RBC) membranes was determined by gas chromatography. Expression of gene encoding for D5D (FADS1), and D6D (FADS2) were evaluated in peripheral blood mononuclear cells by real-time polymerase chain reaction. RESULTS: After 24 months of supplementation, a greater abundance of docosapentaenoic acid (C22:5 n-3), arachidonic acid (C20:4 n-6), adrenic acid (C22:4 n-6), and total n-6 PUFA was found (p = 0.001, p = 0.007, p = 0.033, p = 0.048, respectively). The unsaturated fatty acids/saturated fatty acids ratio, and unsaturation index was increased in the zinc supplemented group at month 24 (p = 0.003 and p  = 0.000, respectively). FADS1 gene was upregulated in the zinc group in relation to placebo at month 12 (p = 0.020). CONCLUSIONS: Supplementation with 30 mg/d elemental zinc during 24 months in patients with T2D had an effect on the composition of RBC membranes increasing PUFA abundance and in turn, improving membrane flexibility. This effect may be mediated by induction of D5D gene expression.

Sistema de porciones de intercambio de alimentos en Chile y el mundo: historia, usos y consideraciones / Food portion exchange lists in Chile and the world: history, uses and considerations

El sistema de porciones de intercambio es una metodología sencilla y rápida que se utiliza para entregar indicaciones alimentarias a usuarios sanos y con patologías. Se realizó una revisión bibliográfica para actualizar conceptos y evaluar la vigencia de su uso y sus aplicaciones. El sistema es ampliamente usado en diferentes países, incluyendo Chile, existiendo distintos tipos de listas de alimentos y preparaciones según la metodología de la definición de las porciones, o bien según diversidad y número de alimentos presentes. Su uso es mayormente con fines asistenciales, siendo también útil en educación comunitaria y docencia. En Chile, los listados de porciones intercambiables fueron publicados el año 1999 y no se han actualizado hasta la fecha. Razones como el cambio en el perfil epidemiológico, mayor acceso a la información y la diversificación de alimentos en la dieta, hace necesario una revisión in extenso de los listados nacionales, con el fin de incorporar mayor diversidad de alimentos y preparaciones típicas a tan masiva y necesaria herramienta técnica, que es fundamental para el profesional Nutricionista y un aporte a la salud y a la cultura de los países.

The food portion exchange list is a simple and fast methodology that is used to give dietary indications to healthy and sick users. A review was carried out to update concepts and evaluate the current state of the exchange list used and their application. The system is widely used in different countries, including Chile, there are different types of foods or preparations exchange lists that use different methodologies for the definition of portions, according to the diversity and number of foods present. Its use is mainly for healthcare purposes, being also useful in community education and college teaching. In Chile, the food portion exchange lists were published in 1999 and have not been updated since. Reasons such as the change in the epidemiological profile, greater access to information and the diversification of foods in the diet, make necessary an in-depth review of the national lists, in order to incorporate a greater diversity of foods and typical preparations. Exchange lists are a necessary technical tool fundamental for nutrition professionals and contribute to the health and culture of countries.

Tratamiento nutricional en úlceras por presión y úlceras venosas / Nutritional treatment in pressure and venous ulcers

RESUMEN Las úlceras por presión (UPP) y úlceras venosas (UV) son frecuentes en adultos y adultos mayores. En Chile un 70% de las heridas crónicas corresponden a UV y la prevalencia de UPP en pacientes hospitalizados es de 28%. El objetivo de esta revisión es analizar la evidencia disponible respecto al tratamiento nutricional de individuos con UPP y UV. Estas úlceras tienen diferentes etiologías, pero ambas provocan un aumento de requerimientos energéticos, proteicos y de micronutrientes. La cicatrización de heridas depende de varios factores como: el tipo de úlcera, estado nutricional, patologías presentes, el tipo de curación y el tratamiento médico. La terapia nutricional establece en la mantención o aumento de peso de estos pacientes, administración adecuada de proteínas y suplementación de energía, proteínas o micronutrientes cuando se presente déficit.

ABSTRACT Pressure and venous ulcers are common in adults and the elderly. In Chile, 70% of chronic wounds correspond to venous ulcers and the prevalence of pressure ulcers in hospitalized patients is 28%. The aim of this review was to analyze the available evidence regarding the nutritional treatment of individuals with pressure and venous ulcers. These ulcers have different etiologies, but both produce an increase in energy, protein, and micronutrient requirements. The healing of wounds depends on several factors such as the type of ulcer, nutritional status, current co-morbidities, healing type and medical treatment. Nutritional therapy should be based on weight maintenance or gain of these patients, adequate administration of proteins and energy supplementation, or protein or micronutrients when a deficiency occurs.

Nutritional Effects of Zinc on Metabolic Syndrome and Type 2 Diabetes: Mechanisms and Main Findings in Human Studies.

Zinc (Zn) plays crucial roles in mammalian metabolism. There is increasing interest about the potential beneficial effects of Zn on the prevention or treatment of non-communicable diseases. This review critically analyzes the information related to the role of Zn on the metabolic syndrome (MetS) as well as type 2 diabetes (T2D), and summarizes the biological basis of these potential effects of Zn. There are several mechanisms by which Zn may help to prevent the development or progression of MetS and T2D, respectively. Zn is involved in both insulin secretion and action in peripheral tissues. Specifically, Zn has insulin-mimetic properties that increase the activity of the insulin signaling pathway. Zn modulates long-chain polyunsaturated fatty acids levels through its action on the absorption of essential fatty acids in the intestine and its subsequent desaturation. Zn is also involved in both the assembly of chylomicrons and lipoproteins as well as their clearance, and thus, plays a role in lipolysis regulation. Finally, Zn has been found to play a role in redox metabolism, and in turn, on blood pressure. The evidence related to the association between Zn status and occurrence of MetS is inconsistent. Although there are several studies reporting an inverse relationship between Zn status or dietary Zn intake and MetS prevalence, others found a direct relationship between Zn status and MetS prevalence. Intervention studies also provide confusing information about this issue, making it hard to reach firm conclusions. Zn as part of the treatment for patients with T2D has been shown to have positive responses in terms of glucose control outcomes, but only among those with Zn deficiency.

Calcium absorption may be affected after either sleeve gastrectomy or Roux-en-Y gastric bypass in premenopausal women: a 2-y prospective study.

Background: Although Roux-en-Y gastric bypass (RYGBP) is known to reduce calcium absorption (CA), the effects of vertical sleeve gastrectomy (SG) and its long-term implications on CA have not yet been studied. Objective: The aim of this study was to evaluate changes in CA and its relation with modifications of bone mineral density (BMD), intakes of calcium and vitamin D, vitamin D status, and parathyroid hormone (PTH) concentrations ≤24 mo after SG and RYGBP, respectively. Design: Twenty-six premenopausal women undergoing SG [mean ± SD body mass index (BMI; kg/m2): 37.3 ± 3.2; age: 34.2 ± 10.2 y] and 32 undergoing RYGBP (BMI: 42.0 ± 4.2; age: 37.3 ± 8.1 y) were studied at baseline (presurgery) and followed up at 12 and 24 mo after surgery. BMD, bone alkaline phosphatase activity, and serum PTH, 25-hydroxyvitamin D [25(OH)D], calcium, magnesium, and phosphorus concentrations were determined. Food and supplement intakes were recorded. CA was measured by using a dual stable isotope method. Results: In premenopausal women, CA was significantly reduced from 36.5% ± 2.0% preoperatively to 21.0% ± 2.3% and 18.8% ± 3.4% at 12 and 24 mo post-SG surgery, respectively. CA also decreased significantly from 41.5% ± 2.8% preoperatively to 27.9% ± 3.8% and 18.5% ± 2.2% 12 and 24 mo after RYGBP, respectively. No difference was found between type of surgery (time × group interaction, P = 0.60). Considering both groups combined, 56.6% of the variance in CA at the 12-mo but not at the 24-mo follow-up was explained by serum PTH and 25(OH)D concentrations, together with vitamin D and calcium intakes. Conclusions: CA was similarly reduced in both SG and RYGBP compared with baseline, and it was not associated with changes in BMD or body weight loss. This reduction in CA could be explained only partially by calcium intake increase. This trial is registered at http://www.isrctn.com as ISRCTN31937503.

Association between zinc nutritional status and glycemic control in individuals with well-controlled type-2 diabetes.

BACKGROUND/OBJECTIVE: Interest in healthy properties of food and nutrients as co-adjuvant in type-2 diabetes therapy has increased in recent years. Zinc supplementation trials have shown improvements in glycemic control in these patients, although it seems dependent on zinc status of the individuals. The objective of this study was to evaluate the relationship between zinc nutritional status and glucose homeostasis in patients with type-2 diabetes. SUBJECTS/METHODS: Eighty patients with well controlled type-2 diabetes were recruited and clinical, anthropometric and dietary evaluations were performed. One week after, insulin sensitivity and beta cell function were assessed by a modified Frequently Sampled Intravenous Glucose Tolerance Test. Zinc status was assessed by plasma zinc and the size of rapidly Exchangeable Zinc Pool (EZP); zinc intake was also determined. Glucagon concentration was evaluated in a subsample of 36 patients. RESULTS: Patients presented a normal zinc status although zinc intake was lower than recommended. Overall, no associations were observed between zinc status and glycemic control markers. Nevertheless, positive correlations were observed between EZP and fasting insulin concentration (ρ = 0.393, p = 0.021) and HOMA-IR (ρ = 0.386, p = 0.024) in women, and between plasma zinc concentration and HbA1c (ρ = 0.342, p = 0.020) in men. CONCLUSIONS: No significant associations were found between zinc status and glycemic control parameters in patients with well-controlled type 2 diabetes and normal zinc status, although low-degree gender-dependent associations were observed. Further research is required to assess the role of zinc status in zinc deficient patients.

Zinc Supplementation Does Not Affect Glucagon Response to Intravenous Glucose and Insulin Infusion in Patients with Well-Controlled Type 2 Diabetes.

Glucagon dysregulation is an essential component in the pathophysiology of type 2 diabetes. Studies in vitro and in animal models have shown that zinc co-secreted with insulin suppresses glucagon secretion. Zinc supplementation improves blood glucose control in patients with type 2 diabetes, although there is little information about how zinc supplementation may affect glucagon secretion. The objective of this study was to evaluate the effect of 1-year zinc supplementation on fasting plasma glucagon concentration and in response to intravenous glucose and insulin infusion in patients with type 2 diabetes. A cross-sectional study was performed after 1-year of intervention with 30 mg/day zinc supplementation or a placebo on 28 patients with type 2 diabetes. Demographic, anthropometric, and biochemical parameters were determined. Fasting plasma glucagon and in response to intravenous glucose and insulin infusion were evaluated. Patients of both placebo and supplemented groups presented a well control of diabetes, with mean values of fasting blood glucose and glycated hemoglobin within the therapeutic goals established by ADA. No significant differences were observed in plasma glucagon concentration, glucagon/glucose ratio or glucagon/insulin ratio fasting, after glucose or after insulin infusions between placebo and supplemented groups. No significant effects of glucose or insulin infusions were observed on plasma glucagon concentration. One-year zinc supplementation did not affect fasting plasma glucagon nor response to intravenous glucose or insulin infusion in well-controlled type 2 diabetes patients with an adequate zinc status.

Efecto del fraccionamiento de la dieta y cantidad de hidratos de carbono en el control metabólico en pacientes con diabetes mellitus tipo 2, sin terapia con insulina / Effect of meal frequency and carbohydrate intake on the metabolic control of patients with type 2 diabetes mellitus

Background: Increasing meal frequency is commonly used in the clinical practice as part of the nutritional treatment of patients with type 2 Diabetes Mellitus (DM2), although its effect on metabolic control parameters is controversial. Aim: To evaluate the association of energy intake, meal frequency, and amount of carbohydrates with fasting plasma glucose and glycosylated hemoglobin in a group of patients with DM2 without insulin therapy. Material and Methods: Dietary intake was evaluated in 60 subjects with DM2 through three-day food records. The meal frequency was estimated establishing the main meal times considering snacks. Results: Meal frequency was 4.7 ± 1.1 times per day. There was a positive association between glycosylated and fasting blood glucose levels (p <0.01). Meal frequency was associated with energy intake (p <0.01). When meal frequency, available carbohydrates and energy intake, body mass index and fasting plasma glucose were analyzed in a multiple linear regression model, fasting blood glucose was the variable that best predicted changes in glycosylated hemoglobin (45.5%). Meal frequency had no association with glycosylated hemoglobin. Conclusions: Meal frequency showed no association with metabolic control parameters in DM2 patients.

[Effect of meal frequency and carbohydrate intake on the metabolic control of patients with type 2 diabetes mellitus]. / Efecto del fraccionamiento de la dieta y cantidad de hidratos de carbono en el control metabólico en pacientes con diabetes mellitus tipo 2, sin terapia con insulina.

BACKGROUND: Increasing meal frequency is commonly used in the clinical practice as part of the nutritional treatment of patients with type 2 Diabetes Mellitus (DM2), although its effect on metabolic control parameters is controversial. AIM: To evaluate the association of energy intake, meal frequency, and amount of carbohydrates with fasting plasma glucose and glycosylated hemoglobin in a group of patients with DM2 without insulin therapy. MATERIAL AND METHODS: Dietary intake was evaluated in 60 subjects with DM2 through three-day food records. The meal frequency was estimated establishing the main meal times considering snacks. RESULTS: Meal frequency was 4.7 ± 1.1 times per day. There was a positive association between glycosylated and fasting blood glucose levels (p <0.01). Meal frequency was associated with energy intake (p <0.01). When meal frequency, available carbohydrates and energy intake, body mass index and fasting plasma glucose were analyzed in a multiple linear regression model, fasting blood glucose was the variable that best predicted changes in glycosylated hemoglobin (45.5%). Meal frequency had no association with glycosylated hemoglobin. CONCLUSIONS: Meal frequency showed no association with metabolic control parameters in DM2 patients.

Micronutrient Deficiencies in Morbidly Obese Women Prior to Bariatric Surgery.

BACKGROUND: Although morbid obesity is related to excess of energy and macronutrient intake, it does not rule out the presence of micronutrient deficiencies. The aim of this study was to evaluate food intake and the prevalence of micronutrient deficiencies in a group of morbidly obese women seeking bariatric surgery. METHODS: A total of 103 morbidly obese women were studied prior to bariatric surgery. Anthropometry and body composition (dual-energy X-ray absorptiometry, DEXA) were performed on all subjects. Energy and nutrient intake was determined by food frequency questionnaire. Blood tests to assess micronutrients status, including plasma iron, ferritin, transferrin, zinc, copper, calcium, phosphorus, hemoglobin, hematocrit, mean corpuscular volume (MCV), and hair zinc, were performed. Folic acid, vitamin B12, vitamin D, and parathyroid hormone (PTH) were also assessed in 66 subjects. RESULTS: Mean energy intake was 2801 ± 970 kcal/day. Carbohydrate, protein, and lipid intake represented 55 ± 9.1, 13.9 ± 3.3, and 32.5 ± 8.2% of total energy intake, respectively. Iron, calcium, and vitamin D intake was below the recommended dietary allowance. The prevalence of nutritional deficiencies were as follows: plasma iron 12.6%, ferritin 8.7%, transferrin 14.6%, plasma zinc 2.9%, calcium 3.3%, phosphorus 2.3%, hemoglobin 7.7%, hematocrit 13.6%, MCV 6.8%, and hair zinc 15.7%. In the subsample, 10.6% had a vitamin B12 deficiency, 71.7% showed low concentrations of vitamin D, and 66% had high PTH levels. No folic acid or copper deficiencies were detected. CONCLUSIONS: Despite high daily energy intake and adequate macronutrient distribution, morbidly obese Chilean women seeking bariatric surgery present with deficient intake of some micronutrients and a high prevalence of micronutrient deficiencies.

Impacto de los ácidos grasos de la dieta sobre el perfil lipídico, la sensibilidad a la insulina y la funcionalidad de las células β pancreáticas en sujetos diabéticos tipo 2 / Impact of dietary fatty acids on lipid profile, insulin sensitivity and functionality of pancreatic β cells in type 2 diabetic subjects

Introducción: la calidad de las grasas podría influir en el control metabólico de los sujetos con diabetes mellitus tipo 2 (DM2). Objetivos: determinar la relación entre la ingesta y la calidad de los ácidos grasos de la dieta con el perfil lipídico, el control metabólico, la funcionalidad de las células β pancreáticas y la sensibilidad a la insulina en sujetos con DM2. Métodos: se estudió a 54 sujetos con DM2, se realizaron determinaciones antropométricas, de composición corporal e ingesta dietética de lípidos, ácidos grasos saturados (AGS), trans, monoinsaturados, poliinsaturados, omega 3, omega 6 y colesterol dietario. Se determinaron los parámetros de laboratorio relacionados con el control metabólico (glicemia de ayuno, hemoglobina glicada, perfil lipídico). La secreción de insulina y la sensibilidad a la insulina se determinaron con el test intravenoso de tolerancia a la glucosa modificado con insulina, basado en el modelo mínimo de Bergman. Resultados: se estudió a 28 hombres y 26 mujeres (edad 55,6 ± 6,8 años; IMC 29,5 ± 3,7 kg/m2 ). Un 48% presentaba c-LDL < 100 mg/dl, el 12,9% de los hombres c-HDL > 40 mg/dl y el 7,4% de las mujeres c-HDL > 50 mg/dl. El 32% consumía > 10% de AGS y > 300 mg/día de colesterol dietario. La ingesta de AGS y el porcentaje de calorías grasas (G%) mostraron una asociación positiva significativa con la resistencia a la insulina y la glicemia. El G% predice en un 84% la variabilidad del c-VLDL. Conclusiones: en los sujetos con DM2, una mayor ingesta de AGS y de grasas se asocia con valores superiores de glicemia y de resistencia a la insulina (AU)

Introduction: the quality of fats could influence the metabolic control of patients with Type 2 Diabetes Mellitus (DM2). Objectives: to determine the relationship between intake and quality of dietary fatty acids to lipid profile, metabolic control, functionality of pancreatic β cells and insulin sensivity in subjects with DM2. Methods: we studied 54 subjects with DM2, anthropometric measurements were performed, body composition and dietary lipid intake, saturated fatty acids (SFA), trans, monounsaturated, polyunsaturated, omega 3, omega 6 and dietary cholesterol. Laboratory parameters related to their metabolic control were determined (fasting blood glucose, glycated hemoglobin, and lipid profile). The insulin secretion and insulin sensitivity was determined with the insulin-modified intravenous glucose tolerance test according to the Bergman’s minimal model. Results: 28 men and 26 women were studied (BMI of 29.5 ± 3.7 kg/m2; age 55.6 ± 6.8 y.), 48% had LDL-C 40 mg/dL and 7.4% of women c-HDL > 50 mg/dL. 32% consumed > 10% of AGS and > 300 mg/day of dietary cholesterol. The SFA intake and percentage of calories from fat (G%) were significantly associated with insulin resistance and fasting plasma glucose concentration. The G% predicted 84% variability on c-VLDL. Conclusions: in patients with DM2 a greater intake of fat and saturated fatty acids it associated with greater fasting glycemia and insulin resistance (AU)

[IMPACT OF DIETARY FATTY ACIDS ON LIPID PROFILE, INSULIN SENSITIVITY AND FUNCTIONALITY OF PANCREATIC ß CELLS IN TYPE 2 DIABETIC SUBJECTS]. / IMPACTO DE LOS ÁCIDOS GRASOS DE LA DIETA SOBRE EL PERFIL LIPÍDICO, LA SENSIBILIDAD A LA INSULINA Y LA FUNCIONALIDAD DE LAS CÉLULAS b PANCREÁTICAS EN SUJETOS DIABÉTICOS TIPO 2.

INTRODUCTION: the quality of fats could influence the metabolic control of patients with Type 2 Diabetes Mellitus (DM2). OBJECTIVES: to determine the relationship between intake and quality of dietary fatty acids to lipid profile, metabolic control, functionality of pancreatic cells and insulin sensivity in subjects with DM2. METHODS: we studied 54 subjects with DM2, anthropometric measurements were performed, body composition and dietary lipid intake, saturated fatty acids (SFA), trans, monounsaturated, polyunsaturated, omega 3, omega 6 and dietary cholesterol. Laboratory parameters related to their metabolic control were determined (fasting blood glucose, glycated hemoglobin, and lipid profile). The insulin secretion and insulin sensitivity was determined with the insulin-modified intravenous glucose tolerance test according to the Bergman's minimal model. RESULTS: 28 men and 26 women were studied (BMI of 29.5 ± 3.7 kg/m2; age 55.6 ± 6.8 y.), 48% had LDL-C < 100 mg/dL, 12.9% of men c-HDL > 40 mg/dL and 7.4% of women c-HDL > 50 mg/dL. 32% consumed > 10% of AGS and > 300 mg/day of dietary cholesterol. The SFA intake and percentage of calories from fat (G%) were significantly associated with insulin resistance and fasting plasma glucose concentration. The G% predicted 84% variability on c-VLDL. CONCLUSIONS: in patients with DM2 a greater intake of fat and saturated fatty acids it associated with greater fasting glycemia and insulin resistance.

Introducción: la calidad de las grasas podría influir en el control metabólico de los sujetos con diabetes mellitus tipo 2 (DM2). Objetivos: determinar la relación entre la ingesta y la calidad de los ácidos grasos de la dieta con el perfil lipídico, el control metabólico, la funcionalidad de las células pancreáticas y la sensibilidad a la insulina en sujetos con DM2. Métodos: se estudió a 54 sujetos con DM2, se realizaron determinaciones antropométricas, de composición corporal e ingesta dietética de lípidos, ácidos grasos saturados (AGS), trans, monoinsaturados, poliinsaturados, omega 3, omega 6 y colesterol dietario. Se determinaron los parámetros de laboratorio relacionados con el control metabólico (glicemia de ayuno, hemoglobina glicada, perfil lipídico). La secreción de insulina y la sensibilidad a la insulina se determinaron con el test intravenoso de tolerancia a la glucosa modificado con insulina, basado en el modelo mínimo de Bergman. Resultados: se estudió a 28 hombres y 26 mujeres (edad 55,6 ± 6,8 años; IMC 29,5 ± 3,7 kg/m2). Un 48% presentaba c-LDL < 100 mg/dl, el 12,9% de los hombres c-HDL > 40 mg/dl y el 7,4% de las mujeres c-HDL > 50 mg/dl. El 32% consumía > 10% de AGS y > 300 mg/día de colesterol dietario. La ingesta de AGS y el porcentaje de calorías grasas (G%) mostraron una asociación positiva significativa con la resistencia a la insulina y la glicemia. El G% predice en un 84% la variabilidad del c-VLDL. Conclusiones: en los sujetos con DM2, una mayor ingesta de AGS y de grasas se asocia con valores superiores de glicemia y de resistencia a la insulina.

Changes in bone mineral density after sleeve gastrectomy or gastric bypass: relationships with variations in vitamin D, ghrelin, and adiponectin levels.

BACKGROUND: A major long-term concern after gastric bypass (GBP) is the risk of osteoporosis; however, little is known about this complication in patients undergoing sleeve gastrectomy (SG). OBJECTIVE: To evaluate changes in bone mineral density (BMD) after GBP and SG, and its relationship with changes in vitamin D, parathyroid hormone (PTH), ghrelin, and adiponectin. METHODS: Twenty-three women undergoing GBP (BMI 42.0 ± 4.2 kg/m2; 37.3 ± 8.1 years) and 20 undergoing SG (BMI 37.3 ± 3.2 kg/m2; 34.2 ± 10.2 years) were studied before and 6 and 12 months after surgery. BMD was measured by dual-energy X-ray absorptiometry. Plasma PTH, 25-hydroxyvitamin D (25-OHD), ghrelin, and adiponectin concentrations were determined. Food as well as calcium and vitamin D supplement intake was recorded. RESULTS: Excess weight loss (mean ± SE), adjusted by baseline excess weight, was 79.1±3.8% and 74.9 ± 4.1% 1 year after GBP and SG, respectively (p = 0.481). Significant reduction in BMD for total body (TB), lumbar spine (LS), and femoral neck (FN) was observed after GBP. In the SG group, reduction in BMD was significant only for TB. Adjusted by baseline BMD, the difference between change in BMD for GBP vs. SG was not significant for TB, LS, or FN. Percent reduction in ghrelin concentration was a main factor related to total BMD loss (GBP group) and LS BMD loss (GBP and SG groups). CONCLUSIONS: One year after gastric bypass, bone mineral density was significantly affected, mainly at the femoral neck. Decreases in bone mineral density were more dramatic among patients who had greater baseline BMD and greater reduction in ghrelin concentrations.

Zinc as a potential coadjuvant in therapy for type 2 diabetes.

BACKGROUND: Type 2 diabetes is highly prevalent in populations having high rates of overweight and obesity. It is a chronic condition responsible for long-term severe dysfunction of several organs, including the kidneys, heart, blood vessels, and eyes. Although there are a number of pharmacologic products in the market to treat insulin resistance and impaired insulin secretion--the most prominent features of this disease--interventions directed at preserving the integrity and function of beta-cells in the long term are less available. The use of some nutrients with important cellular protective roles that may lead to a preservation of beta-cells has not been fully tested; among these, zinc may be an interesting candidate. OBJECTIVE: To assess the potential of zinc supplementation as coadjuvant to diabetes therapy. METHODS: This article reviews the available information on the use of zinc as part of diabetes therapy. RESULTS: Cellular and animal models provide information on the insulin mimetic action of zinc, as well as its role as a regulator of oxidative stress, inflammation, apoptosis, and insulin secretion. Zinc supplementation studies in humans are limited, although some positive effects have been reported; mainly, a modest but significant reduction in fasting glucose and a trend to decreased glycated hemoglobin (HbA1c). CONCLUSIONS: Zinc supplementation may have beneficial effects on glycemic control. Nevertheless, among the studies considered, the vast majority lasted for 6 months or less, suggesting the importance of conducting long-duration studies given the characteristics of type 2 diabetes as a chronic disease.

Heme- and nonheme-iron absorption and iron status 12 mo after sleeve gastrectomy and Roux-en-Y gastric bypass in morbidly obese women.

BACKGROUND: The effect of bariatric surgery on iron absorption is only partially known. OBJECTIVE: The objective was to study the effects of sleeve gastrectomy (SG) and Roux-en-Y gastric bypass (RYGBP) on heme- and nonheme-iron absorption and iron status. DESIGN: Fifty-eight menstruating women were enrolled in this prospective study [mean (±SD) age: 35.9 ± 9.1 y; weight: 101.7 ± 13.5 kg; BMI (in kg/m²): 39.9 ± 4.4]. Anthropometric, body-composition, dietary, and hematologic indexes and heme- and nonheme-iron absorption-using a standardized meal containing 3 mg Fe-were determined before and 12 mo after surgery. Forty-three subjects completed the 12-mo follow-up. Iron supplements were strictly controlled. RESULTS: Heme-iron absorption was 23.9% before and 6.2% 12 mo after surgery (P < 0.0001). Nonheme-iron absorption decreased from 11.1% to 4.7% (P < 0.0001). No differences were observed by type of surgery. Iron intakes from all sources of supplements were 27.9 ± 6.2 mg/d in the SG group and 63.2 ± 21.1 mg/d in the RYGBP group (P < 0.001). Serum ferritin and total-body iron decreased more after RYGBP than after SG. CONCLUSIONS: Iron (heme and nonheme) absorption is markedly reduced after SG and RYGBP. The magnitude of the decrease in heme-iron absorption is greater than that of nonheme iron. The amounts suggested as iron supplements may need to be increased to effectively prevent iron-status impairment.

Changes in ghrelin concentrations one year after resective and non-resective gastric bypass: associations with weight loss and energy and macronutrient intakes.

OBJECTIVE: Ghrelin is a potent stimulator of appetite and synthesized in the stomach. Its role in weight loss after gastric bypass (GBP) is still controversial. The aim of this study was to evaluate the relation between weight loss and food intake and between weight loss and changes in serum ghrelin concentrations 1 y after GBP with resection of the bypassed stomach (R-GBP) and without resection (NR-GBP). METHODS: Of 50 women (37.6 ± 10.2 y old, body mass index 43.8 ± 4.8 kg/m²) with GBP, 26 had R-GBP and 24 had NR-GBP. Body weight, body composition (dual energy x-ray absorptiometry), food intake, and serum ghrelin at baseline and 12 mo after GBP were measured. RESULTS: The percentage of excess weight loss was 68.9 ± 12.8% at 12 mo after GBP. At 12 mo, the decrease of serum ghrelin was greater in the R-GBP group (-25.3 ± 22.5%) compared with the NR-GBP group (+11.2 ± 50.9%, P < 0.005). After adjustment by the baseline excess of body weight, there was a greater percentage of excess weight loss in the R-GBP group only at 6 mo (61.8% versus 54.9%, P = 0.011). After controlling for the baseline intake, a significant lower carbohydrate intake was observed in the R-GBP group 6 mo after surgery (P < 0.05). CONCLUSION: A greater decrease in ghrelin levels was observed only in patients who underwent R-GBP at 12 mo after surgery. This difference was not associated with differences in dietary intakes or weight loss at the same time point. Therefore, the small gastric pouch is probably more important than decreased ghrelin levels in producing long-term weight loss after R-GBP.