[The effect of bemitil on the parameters of lipid peroxidation and nitrogen metabolism in epilepsy in children]. / Vliianie bemitila na pokazateli perekisnogo okisleniia lipidov i azotistogo obmena pri epilepsii u detel.

Epileptic patients given phenobarbital (3 mg/kg, n = 8) or finlepsin (20 mg/kg, n = 7) were found to have a statistically significant increase (p < 0.05) in the parameters of the lipid peroxidation end product malonic dialdehyde in the erythrocytes (3.34 +/- 1.13 mumol/liter) and blood plasma (0.099 +/- 0.04 mumol/liter) in comparison to the control group (n = 9; 1.58 +/- 0.96 mumol/liter and 0.045 +/- 0.02 mumol/liter, respectively). The urea level (6.7 +/- 1.28 mumol/liter) and the ammonia level (31.59 +/- 10.46 mumol/liter) increase were statistically insignificant as compared to the controls (5.76 +/- 0.66 mumol/liter and 26.41 +/- 5.96 mumol/liter, respectively). Bemitil (n = -7) in a dose of 20 mg/kg reduced in 10 days the amount of malonic dialdehyde in the erythrocytes (1.57 +/- 0.61 mumol/liter, p < 0.05) and plasma (0.043 +/- 0.02 mumol/liter, p < 0.05) as well as the amount of urea (3.76 +/- 0.96 mumol/liter,) and ammonia p < 0.05 and ammonia (18.17 +/- 2.02 mumol/liter, p < 0.05) in the blood. A favorable therapeutic effect (lesser frequency of seizures and lesser asthenia of the of the children) was observed at the same time. The frequency of paroxysms reduced to 50% in 4 patients (2 with complex-partial seizures, one with absence, and one with simple-partial seizures) and to 75-% in the fifth patient with complex-partial seizures. The therapeutic effect in the 6th and 7th patients could not be evaluated.

[Androgen regulation of the level of tissue-specific proteins in skeletal muscles during physical exercise]. / Androgennaia reguliatsiia soderzhaniia tkanespetsificheskikh belkov v skeletnykh myshtsakh pri fizicheskikh nagruzkakh.

Administration of testosterone after exercises led to suppression of the hormone production during the anabolic phase within 72-120 hrs of muscles adaptation to physical loading. Content of the androgen receptors was also altered. The supercompensatory phase in content of proteins (myoglobin and aspartate aminotransferase as index) was absent due to an impairing effect of testosterone on muscle adaptation. These data suggest that administration of testosterone could not serve as an adequate model of a body androgenization which was observed in anabolic phase of muscle adaptation to physical exercises.

[Effect of physical exercise on myoglobin and tropomyosin levels in skeletal muscles and myoglobin level in rat blood]. / Vliianie fizicheskoi nagruzki na soderzhanie mioglobina i tropomiozina v skeletnykh myshtsakh i mioglobina v krovi krys.

Single intensive physical exercise caused phase alterations in content of myoglobin and tropomyosin in rat skeletal muscles. Within the first 2-4 hrs of rest concentration of myoglobin and tropomyosin was decreased in muscles by 40-50% (catabolic, urgent step of adaptation). Within the later period of rest (72-120 hrs) content of myoglobin and tropomyosin was increased by 25-30% (anabolic, later step of adaptation) as compared with the control level. Similar phase alterations of these proteins in skeletal muscles were detected in trained animals but they were less distinct. Training of the animals led to elevation in content of the proteins in musculus quadriceps. The content of myoglobin increased from 1.2 +/- 0.1 mg/kg up to 2.6 +/- 0.1 mg/kg and that of tropomyosin from 4.9 +/- 0.1 mg/kg up to 5.5 +/- 0.1 mg/kg as a result of an increase in protein biosynthesis. Incorporation of 14C-leucine into myoglobin was increased 1.7-fold. Concentration of myoglobin in blood of untrained rats was increased 3-fold immediately after physical exercises and--9-fold within 72 hrs of the rest. Content of muscular proteins in blood reflected the response of muscle system to physical exercises and may serve as a criterion in evaluation of various steps of adaptation to physical loading.

The effect of exercises on the content and reception of the steroid hormones in rat skeletal muscles.

Two peaks of hormone concentration in blood and skeletal muscles (SM) were found, immediately after intensive physical exercises (PE) and the late period of rest. Immediately after PE testosterone (T) and estradiol (E2) concentration in SM increased on 36 and 430%, returning to the initial level in 2 h. E2 and androstenedione content in SM increased from 33 +/- 7 to 89 +/- 3 and from 563 +/- 58 to 767 +/- 38 pg/g tissue accordingly in 48 h, returning to the initial level in 72 h. T content increased in blood 3.7-fold in SM 2.2-fold in 72 h after PE. Androgen receptor (AR) contents in SM cytosol increased (on 28%) in 2 h, then returned to the control level and increased again in 72 h (from 0.6 +/- 0.4 to 1.2 +/- 0.2 fmol/mg protein) after PE. Kd did not change significantly and were 0.33 +/- 0.03 nM. The experiments on female rats showed the same tendency, but T increasing was more marked compared to male rats. The obtained results showed that the hormonal regulation of metabolic processes in SM has a cyclic character and is connected with the changing of hormonal content and hormonal SM receptors.

[Steroid levels and androgen receptors in skeletal muscles during adaptation to physical effort]. / Soderzhanie steroidov i retseptsiia androgenov v skeletnykh myshtsakh pri adaptatsii k fizicheskim nagruzkam.

Influence of physical exercises on the content of androstendione, estradiol and testosterone was studied in blood and skeletal muscles. The content of steroids was increased immediately after physical exercises and diminished down to the initial level within 48-72 hrs. Androgen receptors were increased from 0.58 +/- 0.04 to 1.17 +/- 0.17 fmol/mg of protein within 72 hrs after physical exercises in the skeletal muscle cytosol. The increase in the estradiol concentration stimulated the elevation in the androgen receptors content. Effect of estradiol on the content of androgen receptors in skeletal muscles was shown in model experiments using various doses of estradiol-benzoate. Thus, the hormonal status, responsible for metabolism during a body adaptation to physical exercises, depended not only on alterations in the content of steroids but also on the amount of receptors in skeletal muscles within various periods of rests after physical exercises.

[Radioimmunologic determination of androstenedione and the effect of physical training on its levels in human blood and saliva]. / Radioimmunnoe opredelenie androstendiona i vliianie fizicheskikh nagruzok na ego soderzhanie v krovi i sliune cheloveka.

A radioimmunoassay (RIA) of androstenedione in biological fluids was developed. The sensitivity of the method was 30 pg/ml. The efficiency of the standard detection in blood and saliva was 98.2%. The intra- and interassay variations were 5.6% and 12,7%, respectively. Specificity pf the antiserum was determined in cross reactions using 17 steroids. The specificity depended upon the method used, the duration of immunization and the quantity of the antigen. Mathematic methods were used for definition of optimal values of the main parameters and conditions for RIA. 60 different regimes were investigated. The influence of regular physical exercises on androstenedione content in blood and saliva was investigated in 100 athletes. Physical exercises increased androstenedione concentration in men and women blood and saliva. Concentration of androstenedione was increased 1.3- and 2.2-fold in men blood and saliva, respectively, the concentration of androstenedione was increased 1.6-fold in women blood.