Subject(s)
Genital Diseases, Female/complications , Papillomaviridae , Papillomavirus Infections/complications , Tumor Virus Infections/complications , Uterine Cervical Dysplasia/virology , Uterine Cervical Neoplasms/virology , Adult , Female , Genital Diseases, Female/virology , Humans , Male , Neoplasm Invasiveness , Papillomavirus Infections/virology , Tumor Virus Infections/virology , Uterine Cervical Dysplasia/pathology , Uterine Cervical Neoplasms/pathologyABSTRACT
Colonization/infection of the genitals with Mycoplasma, group B Streptococci, and Chlamydia was studied in pregnant women. The course of gestation in women colonized with microorganisms was frequently aggravated by threatened spontaneous abortions, high incidence of hydramnion, late gestoses, pyelonephritis. For the newborns a reduced body mass and increased perinatal morbidity and mortality were characteristic. Transfer of microorganisms to the fetus and newborn was observed in 40-50% of cases and was not always associated with development of a disease. In cases of lethal outcomes of a neonatal disease pneumonia and placentitis were the constant morphological manifestations of the infection. In streptococcal and mycoplasmic infection the inflammation in the placenta was diffuse, involving the decium both in the placenta and in the extraplacental membranes.
Subject(s)
Chlamydia Infections/complications , Fetal Diseases/microbiology , Genital Diseases, Female/microbiology , Infant, Newborn, Diseases/microbiology , Infectious Disease Transmission, Vertical , Mycoplasma Infections/complications , Pregnancy Complications, Infectious/microbiology , Streptococcal Infections/complications , Streptococcus agalactiae/isolation & purification , Chlamydia Infections/microbiology , Chlamydia Infections/transmission , Female , Humans , Infant, Newborn , Mycoplasma Infections/microbiology , Mycoplasma Infections/transmission , Pregnancy , Risk Factors , Streptococcal Infections/microbiology , Streptococcal Infections/transmissionABSTRACT
A quantitative comparison of 4-primer PCR-diagnosticum potentials for detection of cytomegalovirus DNA in urine specimens in different methods for DNA isolation was carried out. Comparatively simple DNA isolation procedures were considered for express diagnosis in clinical conditions. A scheme of successive use of diagnostic preparations was proposed.
Subject(s)
Cytomegalovirus/isolation & purification , Polymerase Chain Reaction/methods , Urine/microbiology , Base Sequence , Cytomegalovirus/genetics , Cytomegalovirus Infections/diagnosis , Cytomegalovirus Infections/urine , Cytomegalovirus Infections/virology , DNA Primers , DNA, Viral/isolation & purification , DNA, Viral/urine , Humans , Infant, Newborn , Molecular Sequence DataABSTRACT
It has been shown that genital Mycoplasma may cause local and generalized diseases particular in parturients and premature neonates. For better laboratory diagnosis of Mycoplasma-induced infections, it is necessary to organize the production of culture media and diagnostic kits.