ABSTRACT
Copaifera oleoresin is one of the most used natural products in popular medicine all over the world. Among other effects (i.e., anti-inflammatory, antinociceptive, microbicidal) one of the most well-known is its wound healing capacity. However, the mechanism by which the oleoresin presents its effect is still not clear. In this study, our aim was to evaluate the wound healing capacity of oleoresin obtained from Copaifera paupera, its mechanism of action and identify its major components. For these purposes, diabetic Swiss Webster mice were topically treated with oleoresin (100, 150 or 200 mg/kg) for 14 consecutive days after an excision was performed in the back of the mice. Cytokines, wound retraction and histological evaluation were conducted at 3, 7 and 10 days (for cytokines); 0, 3, 7, 10 and 14 days (for wound retraction); and 7 and 14 days (for histological evaluation). Our data indicate that oleoresin significantly reduced production of MCP-1 and TNF-α at days 7 and 10 post-excision and increased IL-10 production at both days. All treatments demonstrated an effect similar or higher to that in collagenase-treated mice. Histological evaluations demonstrated that higher dose treatment resulted in better resolution and closure of the wound and higher levels of collagen deposition and indexes of re-epithelialization even when compared with the collagenase-treated group. The treatment with oleoresin from Copaifera paupera demonstrated that it is even better than an ointment routinely used for improvement of wound healing, suggesting this oleoresin as an option for use in diabetic patients.
Subject(s)
Fabaceae/chemistry , Plant Extracts/pharmacology , Wound Healing/drug effects , Animals , Chemokine CCL2/metabolism , Gas Chromatography-Mass Spectrometry , Male , Mice , Tumor Necrosis Factor-alpha/metabolismABSTRACT
Uma revisão acerca dos acontecimentos sobre Bauhinia forficata Link é apresentada dando-se ênfase sobre informações derivadas de suas características botânicas, químicas e farmacológicas. Constam das tradições oral e escrita do Brasil vários exemplos de vegetais usados como medicinais entre os quais vários empregados como hipoglicemiantes e como diuréticos. Entre esses últimos destaca-se Bauhinia forficata Link. Inúmeros trabalhos científicos põem em evidência suas propriedades hipoglicemiantes, diuréticas, bem como sua possível atividade antiinflamatória e analgésica. Em sua composição foi evidenciada presença de beta-sitosterol e quempferirina, além de glicósidos do sitosterol. Estigmasterol de campesterol também estão presentes, além de abordagem fitoquímica indicar a presença de óleo essencial, alcalóides, taninos e mucilagens.
