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1.
Food Chem ; 150: 27-33, 2014 May 01.
Article in English | MEDLINE | ID: mdl-24360415

ABSTRACT

The taste of different enantiomeric forms of amino acids has been deeply investigated because it represents the most impressive case of correlation between stereochemistry and flavour. Herein, we aimed to elucidate the molecular activity of d- and l-amino acids using an in vitro system based on a cellular model overexpressing sweet and bitter receptors, and to analyse the correlation between in vitro and sensory studies. With our work we demonstrated specific enantiomeric activities for several amino acids on TAS1R2-TAS1R3 sweet receptor. Moreover, we proved interaction of tryptophan and phenylalanine with a specific group of TAS2Rs bitter receptors, confirming and improving the results recently obtained in the tasting of amino acids. In addition, we provide the first systematic analysis of l- and d-amino acid actions on the sweet heterodimeric receptor.


Subject(s)
Amino Acids/metabolism , Receptors, G-Protein-Coupled/metabolism , Amino Acid Sequence , Amino Acids/chemistry , Dimerization , Humans , Molecular Sequence Data , Protein Binding , Receptors, G-Protein-Coupled/chemistry , Receptors, G-Protein-Coupled/genetics , Taste
2.
Food Chem ; 141(3): 2044-51, 2013 Dec 01.
Article in English | MEDLINE | ID: mdl-23870926

ABSTRACT

Transient receptor potential (TRP) channels represent interesting molecular target structures involved in a number of different physiological and pathophysiological systems. In particular, TRPA1 channel is involved in nociception and in sensory perception of many pungent chemesthetic compounds, which are widespread in spices and food plants, including Perilla frutescens. A natural compound from P. frutescens (isoegomaketone) and 16 synthetic derivatives of perillaketone have been prepared and tested in vitro on rTRPA1 expressed in HEK293 cells and their potency, efficacy and desensibilisation activity measured. Most derivatives proved to be high potency agonists of TRPA1, with a potency higher than most natural agonists reported in the literature. These furylketones derivatives, represent a new class of chemical structures active on TRPA1 with many potential applications in the agrifood and pharmaceutical industry.


Subject(s)
Monoterpenes/chemistry , Perilla/chemistry , Plant Extracts/chemistry , Transient Receptor Potential Channels/agonists , Animals , HEK293 Cells , Humans , Kinetics , Molecular Structure , Monoterpenes/chemical synthesis , Plant Extracts/chemical synthesis , Rats , Transient Receptor Potential Channels/chemistry
3.
J Agric Food Chem ; 46(1): 314-317, 1998 Jan 19.
Article in English | MEDLINE | ID: mdl-10554239

ABSTRACT

The s-triazines terbutryn and terbuthylazine are currently used in Italy for weed control. A possible reaction of these compounds in the environment is N-nitrosation. Experiments performed in 10 mM CaCl(2) as a model of soil water indicated that N-nitrosation is favored only at low pH values and that the N-nitroso derivatives are fairly stable. In the presence of soil either parent compounds or N-nitroso derivatives are strongly adsorbed. These results seem to indicate that the possibility of formation of N-nitrosoterbuthylazine and N-nitrosoterbutryn in common agricultural soil is very remote.

4.
J Chromatogr ; 446: 179-85, 1988 Jul 27.
Article in English | MEDLINE | ID: mdl-3209654

ABSTRACT

A preparative-scale enrichment of the allergenic components of the pollen extract of Parietaria judaica, which grow all over the Mediterranean area, has been obtained by high-performance liquid chromatography, operating in the ion-exchange mode at pH 7 with a curvilinear ionic-strength gradient.


Subject(s)
Allergens/analysis , Plant Extracts/analysis , Pollen/analysis , Chromatography, High Pressure Liquid , Isoelectric Focusing , Radioallergosorbent Test , Ultrafiltration
5.
J Chromatogr ; 444: 209-18, 1988 Jul 01.
Article in English | MEDLINE | ID: mdl-3204131

ABSTRACT

Size-exclusion and ion-exchange high-performance liquid chromatography were used to monitor the presence of polymeric aggregates and of families of allergens of similar isoelectric point in Parietaria judaica pollen extracts. A radio-allergo-sorbent test and chromatofocusing were used for the analysis of individual fractions. This allows the detection of two groups of allergens, having pI of 4.7 and greater than 5.7, respectively, and shows the effect of dialysis or ultrafiltration on the distribution of allergens.


Subject(s)
Allergens/analysis , Plant Extracts/analysis , Pollen/analysis , Chromatography, Gel , Chromatography, High Pressure Liquid , Evaluation Studies as Topic , Isoelectric Focusing , Radioallergosorbent Test
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