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1.
J Trop Pediatr ; 59(4): 317-20, 2013 Aug.
Article in English | MEDLINE | ID: mdl-23598894

ABSTRACT

The Vi capsular polysaccharide (ViPS) protects Salmonella enterica subspecies enterica serotype Typhi (S.Typhi) in vivo by multiple mechanisms. Recent microbiological reports from typhoid endemic countries suggest that acapsulate S.Typhi may occur in nature and contribute to clinical typhoid fever that is indistinguishable from disease caused by capsulate strains. The prevalence and genetic basis of ViPS-negative S.Typhi isolates in children from Kathmandu, Nepal, were tested in 68 isolates. Although 5.9% of isolates tested negative for capsular expression by slide agglutination tests, a novel multiplex PCR assay and individual PCR analyses demonstrated the presence of all 14 genes responsible for the synthesis, transportation and regulation of the ViPS. These data suggest that phenotypically acapsulate S.Typhi may not have a genetic basis for the same.


Subject(s)
Genes, Bacterial , Salmonella typhi/genetics , Salmonella typhi/isolation & purification , Typhoid Fever/epidemiology , Child , Genome, Bacterial , Humans , Infant , Mutation , Nepal/epidemiology , Phenotype , Polymerase Chain Reaction , Polysaccharides, Bacterial/metabolism , Prevalence , Salmonella typhi/immunology , Typhoid Fever/blood , Typhoid Fever/microbiology
2.
Clin Vaccine Immunol ; 16(10): 1413-9, 2009 Oct.
Article in English | MEDLINE | ID: mdl-19710294

ABSTRACT

Typhoid fever is a major public health problem in developing countries, conservatively estimated to occur in 17 million cases and be responsible for 200,000 deaths annually. We investigated the acquisition of natural immunity to Salmonella enterica serovar Typhi in a region where typhoid is endemic by testing sera from an age-stratified sample of 210 healthy participants in Kathmandu, Nepal, for bactericidal activity toward S. Typhi and for anti-Vi capsular polysaccharide antibodies. Bactericidal titers in children were significantly lower than those in newborns and adults (P < 0.0001). Anti-S. Typhi bactericidal geometric mean titers were age dependent, increasing 10-fold during childhood. Anti-Vi polysaccharide antibody geometric mean concentrations were also lower in children than in adults. Data presented here indicate the possibility of a relationship between low levels of bactericidal activity toward S. Typhi in serum and susceptibility to disease, as observed for other polysaccharide-encapsulated bacteria. Bactericidal antibody may be a marker of protective immunity against S. Typhi.


Subject(s)
Antibodies, Bacterial/blood , Blood Bactericidal Activity , Salmonella typhi/immunology , Typhoid Fever/epidemiology , Typhoid Fever/immunology , Adolescent , Adult , Age Factors , Aged , Antigens, Bacterial , Child , Child, Preschool , Cross-Sectional Studies , Female , Fetal Blood/immunology , Humans , Immunity, Humoral , Immunity, Innate , Infant , Infant, Newborn , Male , Middle Aged , Nepal/epidemiology , Polysaccharides, Bacterial/immunology , Pregnancy , Young Adult
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