ABSTRACT
STATEMENT OF PROBLEM: Dental implants obtained by additive manufacturing may present changes in the microbiome formed. However, studies profiling the microbial communities formed on Ti-6Al-4V are lacking. PURPOSE: The purpose of this in situ study was to characterize the profile of the microbial communities formed on Ti-6Al-4V disks produced by additive manufacturing and machining. MATERIAL AND METHODS: Titanium disks produced by additive manufacturing (AMD) and machining (UD) were housed in the buccal region of removable intraoral devices. These devices containing both disks were used by eight participants for 96 hours. After every 24 hours of intraoral exposure, the biofilm that had formed on the disks was collected. The 16S rRNA genes from each specimen were amplified and sequenced with the Miseq Illumina instrument and analyzed. Total microbial quantification was evaluated by analysis of variance-type statistics using the nparLD package. The Wilcoxon test was used to evaluate alpha diversity (α=.05). RESULTS: A difference was found in the microbial communities formed on additively manufactured and machined disks, with a reduction in operational taxonomic units (OTUs) for the AMD group compared with the UD group. Firmicutes and Proteobacteria were the most abundant phyla. Of the 1256 genera sequenced, Streptococcus predominated on both disks. CONCLUSIONS: The microbiome of the biofilm formed on the Ti-6Al-4V disks was significantly influenced by the fabrication method. The AMD disks showed lower total microbial counts than the UD disks.
ABSTRACT
This work presents a new eddy current testing array probe and readout electronics that target the layer-wise quality control in powder bed fusion metal additive manufacturing. The proposed design approach brings important benefits to the sensors' number scalability, exploring alternative sensor elements and minimalist signal generation and demodulation. Small-sized, commercially available surface-mounted technology coils were evaluated as an alternative to usually employed magneto-resistive sensors, demonstrating low cost, design flexibility, and easy integration with the readout electronics. Strategies to minimize the readout electronics were proposed, considering the specific characteristics of the sensors' signals. An adjustable single phase coherent demodulation scheme is proposed as an alternative to traditional in-phase and quadrature demodulation provided that the signals under measurement showed minimal phase variations. A simplified amplification and demodulation frontend using discrete components was employed together with offset removal, vector amplification, and digitalization implemented within the microcontrollers' advanced mixed signal peripherals. An array probe with 16 sensor coils and a 5 mm pitch was materialized together with non-multiplexed digital readout electronics, allowing for a sensor frequency of up to 1.5 MHz and digitalization with 12 bits resolution, as well as a 10 kHz sampling rate.
