ABSTRACT
HLA-A*02:653 differs from A*02:01:01:01 by a C to T substitution in exon 2.
Subject(s)
Alleles , HLA-A Antigens/genetics , Base Sequence , Exons/genetics , Female , Humans , ItalyABSTRACT
The novel allele HLA-A*03:275N differs from HLA-A*03:01:01:01 by 1 nucleotide substitutions in exon 2.
Subject(s)
Exons , HLA-A3 Antigen/genetics , Mutation, Missense , HumansABSTRACT
The novel allele DQA1*01:15N differs from DQA1*01:03:01:01 by 1 nucleotide substitutions in exon 2.
Subject(s)
Alleles , HLA-DQ Antigens/genetics , Humans , ItalyABSTRACT
HLA-C*07:555 differs from C*07:01:01:01 by a C to G substitution in exon 2.
Subject(s)
Alleles , Exons , HLA-C Antigens/genetics , Polymorphism, Single Nucleotide , Unrelated Donors , Amino Acid Substitution , Base Sequence , Codon/chemistry , Gene Expression , Genotype , HLA-C Antigens/immunology , Hematopoietic Stem Cell Transplantation , Histocompatibility Testing , Humans , Polymerase Chain Reaction , Sequence Alignment , Sequence Analysis, DNAABSTRACT
HLA-C*02:106 allele differs from C*02:02:02:01 by a C to T substitution in exon 4.
Subject(s)
Alleles , Exons , HLA-C Antigens/genetics , Point Mutation , Unrelated Donors , Amino Acid Substitution , Base Sequence , Cloning, Molecular , Gene Expression , Genotype , HLA-C Antigens/immunology , Hematopoietic Stem Cell Transplantation , Histocompatibility Testing , Humans , Polymerase Chain Reaction , Sequence Alignment , Sequence Analysis, DNAABSTRACT
The simultaneous typing of five-HLA loci at high resolution and the availability of pedigree data allowed us to characterize extended five-locus phased haplotypes in 124 Nigerian families and to compare the observed frequencies with those expected by an expectation-maximization algorithm for unphased data. Despite the occurrence of some frequent alleles at each locus (e.g. B*53:01, which is assumed to protect against Plasmodium falciparum), as many as 82% of the sampled individuals carry two unique five-locus haplotypes and only three extended haplotypes with frequency above 1% exhibit significant linkage disequilibrium. Although preliminary, these results reveal an extreme level of HLA diversity in the Nigerian population, which reflects both its multi-ethnic composition and the very ancient demographic history of African populations.
Subject(s)
HLA-A Antigens/genetics , HLA-B Antigens/genetics , HLA-C Antigens/genetics , HLA-DQ beta-Chains/genetics , HLA-DRB1 Chains/genetics , Haplotypes , Linkage Disequilibrium , Alleles , Family , Gene Expression , Gene Frequency , Genetic Variation , Genetics, Population , HLA-A Antigens/immunology , HLA-B Antigens/immunology , HLA-C Antigens/immunology , HLA-DQ beta-Chains/immunology , HLA-DRB1 Chains/immunology , Histocompatibility Testing , Humans , Nigeria , PedigreeABSTRACT
The novel DRB1*01:16 allele differs for G to T substitution at position 595 from DRB3*01:01P.
Subject(s)
Alleles , HLA-DRB3 Chains/genetics , Hematopoietic Stem Cell Transplantation , Point Mutation , Amino Acid Substitution , Base Sequence , Child , Cloning, Molecular , Codon , Exons , Family , Genotype , HLA-DRB3 Chains/immunology , Histocompatibility Testing , Humans , Italy , Male , Molecular Sequence Data , Sequence Alignment , Sequence Analysis, DNA , Transplant RecipientsABSTRACT
The information regarding the probability of finding a matched unrelated donor (MUD) within a relatively short time is crucial for the success of hematopoietic stem cell transplantation (HSCT), particularly in patients with malignancies. In this study, we retrospectively analyzed 315 Italian patients who started a search for a MUD, in order to assess the distribution of human leukocyte antigen (HLA) alleles and haplotypes in this population of patients and to evaluate the probability of finding a donor. Comparing two groups of patients based on whether or not a 10/10 HLA-matched donor was available, we found that patients who had a fully-matched MUD possessed at least one frequent haplotype more often than the others (45.6% vs 14.3%; P = 0.000003). In addition, analysis of data pertaining to the HLA class I alleles distribution showed that, in the first group of patients, less common alleles were under-represented (20.2% vs 40.0%; P = 0.006). Therefore, the presence of less frequent alleles represents a negative factor for the search for a potential compatible donor being successful, whereas the presence of one frequent haplotype represents a positive predictive factor. Antigenic differences between patient and donor observed at C and DQB1 loci, were mostly represented by particular B/C or DRB1/DQB1 allelic associations. Thus, having a particular B or DRB1 allele, linked to multiple C or DQB1 alleles, respectively, might be considered to be associated with a lower probability of a successful search. Taken together, these data may help determine in advance the probability of finding a suitable unrelated donor for an Italian patient.
Subject(s)
Donor Selection , HLA Antigens/genetics , Hematopoietic Stem Cell Transplantation , Tissue Donors , Alleles , Gene Frequency/genetics , Genetic Loci/genetics , Haplotypes/genetics , Humans , Italy , Unrelated DonorsSubject(s)
Alleles , Exons , HLA-B Antigens/genetics , Multiple Myeloma/genetics , Point Mutation , Base Sequence , Codon , Family , HLA-B Antigens/immunology , Histocompatibility Testing , Humans , Italy , Male , Middle Aged , Molecular Sequence Data , Multiple Myeloma/immunology , Multiple Myeloma/therapy , Sequence Alignment , Sequence Analysis, DNAABSTRACT
The new allele, officially named HLA DRB1*14:129, differs from HLA DRB1*14:54 in exon 2.
Subject(s)
Alleles , HLA-DRB1 Chains/genetics , Point Mutation , Base Sequence , Exons , HLA-DRB1 Chains/immunology , Histocompatibility Testing , Humans , Molecular Sequence Data , Stem Cell Transplantation , Tissue DonorsSubject(s)
Exons/genetics , HLA-DP beta-Chains/genetics , Hematopoietic Stem Cell Transplantation , Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics , Base Sequence , Child , Family , Histocompatibility Testing , Humans , Male , Molecular Sequence Data , Polymorphism, Genetic , Precursor Cell Lymphoblastic Leukemia-Lymphoma/therapy , Sequence Alignment , Transplantation , VenezuelaABSTRACT
HLA-A*03:143 has one nucleotide change from A*03:01: 01:01 at nt 406 from G to C, resulting in an amino acid change at codon 112 of exon 3 from Gly to Arg.
Subject(s)
HLA-A3 Antigen/genetics , Alleles , Amino Acid Substitution , Base Sequence , DNA/genetics , Exons , Female , Genotyping Techniques , Haplotypes , Histocompatibility Testing , Humans , Italy , Male , Molecular Sequence Data , Pedigree , Polymorphism, Single Nucleotide , Sequence Homology, Nucleic AcidABSTRACT
The new allele shows one nucleotide change from C*07:02:01:01 at 351 nt from C to A, resulting in an amino acid change at codon 93 of exon 3 from H to Q.
Subject(s)
Alleles , Exons/genetics , HLA-C Antigens/genetics , Mutation/genetics , Amino Acid Sequence , DNA Probes, HLA/genetics , Hematopoietic Stem Cell Transplantation , Histocompatibility Testing , Humans , Italy , Molecular Sequence Data , Polymorphism, Single Nucleotide , Sequence Analysis, DNA , Tissue DonorsABSTRACT
Human leukocyte antigen (HLA) class I sequence-based typing (SBT) for hematopoietic unrelated donor searching in an Italian Caucasian patient showed the presence of a novel HLA-A allele defined as A*31:48. HLA-A*31:48 has one nucleotide change from A*31:01:02 at nt 727 from C to T, resulting in an amino acid change at codon 219 of exon 4 from Arg to Trp.
Subject(s)
Alleles , Amino Acid Substitution , HLA-A Antigens/genetics , Mutation, Missense , Adult , Anemia, Aplastic/genetics , Anemia, Aplastic/therapy , Female , HumansABSTRACT
New allele B*0769 showed one nucleotide difference with B*0732 at codon 272 (TTC-->TGC).
Subject(s)
Alleles , HLA-B Antigens/genetics , Nuclear Family , Adolescent , Amino Acid Sequence , Amino Acid Substitution , Base Sequence , Cysteine/metabolism , DNA Primers/genetics , Exons , Genetic Variation , HLA-B7 Antigen , Haplotypes , Histocompatibility Testing/methods , Humans , Iran , Male , Molecular Sequence Data , Polymerase Chain Reaction , Sequence Analysis, DNA , Sequence Homology, Nucleic Acid , Terminology as TopicABSTRACT
We report here the identification of a novel DRB1*11 allele, DRB1*1144, identified during sequence-based HLA-DRB1 typing. Molecular cloning and direct sequencing confirmed that the new allele is identical to DRB1*110401 at exon 2, except for a single nucleotide substitution (GTG-->GCG) changing codon 38 from Valine to Alanine.