ABSTRACT
As part of an undergraduate microbiology course, a yellow-orange-pigmented, Gram-staining negative, rod-shaped, non-motile bacterial strain was isolated from a glass tank housing several red-spotted newts (Notophthalmus viridescens). The sequence of the 16S rRNA gene of this strain, designated KM(T), was 97.4-98.0â% similar to those of the type strains of Chryseobacterium luteum, C. shigense and C. vrystaatense, while the similarity levels for protein-coding genes were less than 94.7â% for rpoB, less than 92.1â% for groEL and less than 87.1â% for gyrB. These values are lower than for many other established distinct species. Polyphasic characterization and comparison to these relatives revealed that strain KM(T) was similar to other Chryseobacterium strains in that it contained MK-6 as its major respiratory quinone and phosphatidylethanolamine as the most abundant polar lipid, produced flexirubin-type pigments, oxidase and catalase and primarily contained the fatty acids iso-C15â:â0, iso-C17â:â1ω9c, iso-C17â:â0 3-OH and summed feature 3 (comprising C16â:â1ω6c and/or C16â:â1ω7c). Based on the results of this study, strain KM(T) represents a novel species, for which the name Chryseobacterium angstadtii sp. nov. is proposed. The type strain is KM(T) (â=âATCC BAA-2160(T)â=âNRRL B-59516(T)â=âKCTC 23297(T)).
Subject(s)
Chryseobacterium/classification , Phylogeny , Salamandridae , Animals , Bacterial Typing Techniques , Base Composition , Chaperonin 60/genetics , Chryseobacterium/genetics , Chryseobacterium/isolation & purification , DNA Gyrase/genetics , DNA, Bacterial/genetics , Fatty Acids/chemistry , Molecular Sequence Data , Phosphatidylethanolamines/chemistry , Polyenes/chemistry , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Vitamin K 2/analogs & derivatives , Vitamin K 2/chemistryABSTRACT
As part of an undergraduate microbiology course, a yellow-orange pigmented, Gram-staining negative, rod-shaped, non-motile bacterial strain, designated CTM(T), was isolated from a creek in North-central Pennsylvania during the winter of 2006. The 16S rRNA gene sequence of the strain showed ~97â% similarity to that of Chryseobacterium soldanellicola PSD1-4(T) and Chryseobacterium soli JS6-6(T), while the protein-coding gyrB gene sequence of strain CTM(T) showed <87â% similarity to those of its two closest relatives. Using a polyphasic approach, strain CTM(T) was characterized and compared to these and other closely related species of the genus Chryseobacterium. Strain CTM(T) was similar to other strains of the genus Chryseobacterium in that it contained MK-6 as its major respiratory quinone, produced flexirubin-type pigments, oxidase and catalase, hydrolysed DNA, gelatin and aesculin and contained the fatty acids iso-C15:0, iso-C17:1ω9c, iso-C17:0 3-OH and summed feature 3 (C16:1ω6c, C16:1ω7c and/or iso-C15:0 2-OH). Based on the results of this study, strain CTM(T) represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium piperi sp. nov. is proposed. The type strain is CTM(T) (â=âATCC BAA-1782(T) â=âCCUG 57707(T) â=âJCM 15960(T) â=âDSM 22249(T) â=âKCTC 23267(T)).
