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1.
Nature ; 607(7920): 790-798, 2022 07.
Article in English | MEDLINE | ID: mdl-35768505

ABSTRACT

Ageing is intimately connected to the induction of cell senescence1,2, but why this is so remains poorly understood. A key challenge is the identification of pathways that normally suppress senescence, are lost during ageing and are functionally relevant to oppose ageing3. Here we connected the structural and functional decline of ageing tissues to attenuated function of the master effectors of cellular mechanosignalling YAP and TAZ. YAP/TAZ activity declines during physiological ageing in stromal cells, and mimicking such decline through genetic inactivation of YAP/TAZ in these cells leads to accelerated ageing. Conversely, sustaining YAP function rejuvenates old cells and opposes the emergence of ageing-related traits associated with either physiological ageing or accelerated ageing triggered by a mechano-defective extracellular matrix. Ageing traits induced by inactivation of YAP/TAZ are preceded by induction of tissue senescence. This occurs because YAP/TAZ mechanotransduction suppresses cGAS-STING signalling, to the extent that inhibition of STING prevents tissue senescence and premature ageing-related tissue degeneration after YAP/TAZ inactivation. Mechanistically, YAP/TAZ-mediated control of cGAS-STING signalling relies on the unexpected role of YAP/TAZ in preserving nuclear envelope integrity, at least in part through direct transcriptional regulation of lamin B1 and ACTR2, the latter of which is involved in building the peri-nuclear actin cap. The findings demonstrate that declining YAP/TAZ mechanotransduction drives ageing by unleashing cGAS-STING signalling, a pillar of innate immunity. Thus, sustaining YAP/TAZ mechanosignalling or inhibiting STING may represent promising approaches for limiting senescence-associated inflammation and improving healthy ageing.


Subject(s)
Aging , Membrane Proteins , Nucleotidyltransferases , Stromal Cells , Transcriptional Coactivator with PDZ-Binding Motif Proteins , YAP-Signaling Proteins , Actin-Related Protein 2/metabolism , Aging/metabolism , Cellular Senescence , Extracellular Matrix , Healthy Aging , Immunity, Innate , Lamin Type B/metabolism , Mechanotransduction, Cellular/genetics , Membrane Proteins/metabolism , Nuclear Envelope/metabolism , Nucleotidyltransferases/metabolism , Signal Transduction , Stromal Cells/metabolism , Transcriptional Coactivator with PDZ-Binding Motif Proteins/antagonists & inhibitors , Transcriptional Coactivator with PDZ-Binding Motif Proteins/metabolism , YAP-Signaling Proteins/antagonists & inhibitors , YAP-Signaling Proteins/metabolism
2.
Cell Stress ; 5(11): 167-172, 2021 Nov.
Article in English | MEDLINE | ID: mdl-34782888

ABSTRACT

Dysregulated gene expression is intrinsic to cell transformation, tumorigenesis and metastasis. Cancer-specific gene-expression profiles stem from gene regulatory networks fueled by genetic and epigenetic defects, and by abnormal signals of the tumor microenvironment. These oncogenic signals ultimately engage the transcriptional machinery on the cis -regulatory elements of a host of effector genes, through recruitment of transcription factors (TFs), co-activators and chromatin regulators. That said, whether gene-expression in cancer cells is the chaotic product of myriad regulations or rather a relatively ordered process orchestrated by few TFs (master regulators) has long remained enigmatic. Recent work on the YAP/TAZ co-activators has been instrumental to break new ground into this outstanding issue, revealing that tumor cells hijack growth programs that are active during development and regeneration through engagement of a small set of interconnected TFs and their nuclear partners.

3.
Sci Rep ; 11(1): 22668, 2021 11 22.
Article in English | MEDLINE | ID: mdl-34811382

ABSTRACT

In spite of tremendous advances made in the comprehension of mechanotransduction, implementation of mechanobiology assays remains challenging for the broad community of cell biologists. Hydrogel substrates with tunable stiffness are essential tool in mechanobiology, allowing to investigate the effects of mechanical signals on cell behavior. A bottleneck that slows down the popularization of hydrogel formulations for mechanobiology is the assessment of their stiffness, typically requiring expensive and sophisticated methodologies in the domain of material science. Here we overcome such barriers offering the reader protocols to set-up and interpret two straightforward, low cost and high-throughput tools to measure hydrogel stiffness: static macroindentation and micropipette aspiration. We advanced on how to build up these tools and on the underlying theoretical modeling. Specifically, we validated our tools by comparing them with leading techniques used for measuring hydrogel stiffness (atomic force microscopy, uniaxial compression and rheometric analysis) with consistent results on PAA hydrogels or their modification. In so doing, we also took advantage of YAP/TAZ nuclear localization as biologically validated and sensitive readers of mechanosensing, all in all presenting a suite of biologically and theoretically proven protocols to be implemented in most biological laboratories to approach mechanobiology.

4.
Nat Cancer ; 2(2): 174-188, 2021 02.
Article in English | MEDLINE | ID: mdl-33644767

ABSTRACT

Glioblastoma (GBM) is a devastating human malignancy. GBM stem-like cells (GSCs) drive tumor initiation and progression. Yet, the molecular determinants defining GSCs in their native state in patients remain poorly understood. Here we used single cell datasets and identified GSCs at the apex of the differentiation hierarchy of GBM. By reconstructing the GSCs' regulatory network, we identified the YAP/TAZ coactivators as master regulators of this cell state, irrespectively of GBM subtypes. YAP/TAZ are required to install GSC properties in primary cells downstream of multiple oncogenic lesions, and required for tumor initiation and maintenance in vivo in different mouse and human GBM models. YAP/TAZ act as main roadblock of GSC differentiation and their inhibition irreversibly lock differentiated GBM cells into a non-tumorigenic state, preventing plasticity and regeneration of GSC-like cells. Thus, GSC identity is linked to a key molecular hub integrating genetics and microenvironmental inputs within the multifaceted biology of GBM.


Subject(s)
Brain Neoplasms , Glioblastoma , Animals , Brain Neoplasms/genetics , Carcinogenesis/pathology , Cell Plasticity , Glioblastoma/genetics , Humans , Mice , Neoplastic Stem Cells/pathology , Single-Cell Analysis
5.
Nat Mater ; 19(7): 797-806, 2020 07.
Article in English | MEDLINE | ID: mdl-32066931

ABSTRACT

Defining the interplay between the genetic events and microenvironmental contexts necessary to initiate tumorigenesis in normal cells is a central endeavour in cancer biology. We found that receptor tyrosine kinase (RTK)-Ras oncogenes reprogram normal, freshly explanted primary mouse and human cells into tumour precursors, in a process requiring increased force transmission between oncogene-expressing cells and their surrounding extracellular matrix. Microenvironments approximating the normal softness of healthy tissues, or blunting cellular mechanotransduction, prevent oncogene-mediated cell reprogramming and tumour emergence. However, RTK-Ras oncogenes empower a disproportional cellular response to the mechanical properties of the cell's environment, such that when cells experience even subtle supra-physiological extracellular-matrix rigidity they are converted into tumour-initiating cells. These regulations rely on YAP/TAZ mechanotransduction, and YAP/TAZ target genes account for a large fraction of the transcriptional responses downstream of oncogenic signalling. This work lays the groundwork for exploiting oncogenic mechanosignalling as a vulnerability at the onset of tumorigenesis, including tumour prevention strategies.


Subject(s)
Cellular Reprogramming/physiology , Extracellular Matrix/physiology , Oncogenes/physiology , Animals , Biomechanical Phenomena , Cell Line, Tumor , Female , Gene Expression Regulation , Humans , Mammary Glands, Human/cytology , Mammary Glands, Human/metabolism , Mice , Mice, Inbred Strains , Mice, Knockout , Microscopy/methods , Oncogenes/genetics , Pancreas/cytology , Sequence Analysis, RNA
7.
Proc Natl Acad Sci U S A ; 116(36): 17848-17857, 2019 09 03.
Article in English | MEDLINE | ID: mdl-31416916

ABSTRACT

Autophagy, besides ensuring energy metabolism and organelle renewal, is crucial for the biology of adult normal and cancer stem cells. However, it remains incompletely understood how autophagy connects to stemness factors and the nature of the microenvironmental signals that pattern autophagy in different cell types. Here we advance in these directions by reporting that YAP/TAZ transcriptionally control autophagy, being critical for autophagosomal degradation into autolysosomes. YAP/TAZ are downstream effectors of cellular mechanotransduction and indeed we found that cell mechanics, dictated by the physical property of the ECM and cytoskeletal tension, profoundly impact on autophagic flux in a YAP/TAZ-mediated manner. Functionally, by using pancreatic and mammary organoid cultures, we found that YAP/TAZ-regulated autophagy is essential in normal cells for YAP/TAZ-mediated dedifferentiation and acquisition of self-renewing properties. In tumor cells, the YAP/TAZ-autophagy connection is key to sustain transformed traits and for acquisition of a cancer stem cell state by otherwise more benign cells. Mechanistically, YAP/TAZ promote autophagic flux by directly promoting the expression of Armus, a RAB7-GAP required for autophagosome turnover and whose add-back rescues autophagy in YAP/TAZ-depleted cells. These findings expand the influence of YAP/TAZ mechanotransduction to the control of autophagy and, vice versa, the role of autophagy in YAP/TAZ biology, and suggest a mechanism to coordinate transcriptional rewiring with cytoplasmic restructuring during cell reprogramming.


Subject(s)
Autophagy , Cell Cycle Proteins/metabolism , Cell Plasticity , Mechanotransduction, Cellular , Transcription Factors/metabolism , Acyltransferases , Adaptation, Physiological , Animals , Autophagosomes , Humans , Lysosomes/metabolism , Protein Binding , Proteolysis
8.
Nature ; 563(7730): 265-269, 2018 11.
Article in English | MEDLINE | ID: mdl-30401838

ABSTRACT

Inactivation of ARID1A and other components of the nuclear SWI/SNF protein complex occurs at very high frequencies in a variety of human malignancies, suggesting a widespread role for the SWI/SNF complex in tumour suppression1. However, the underlying mechanisms remain poorly understood. Here we show that ARID1A-containing SWI/SNF complex (ARID1A-SWI/SNF) operates as an inhibitor of the pro-oncogenic transcriptional coactivators YAP and TAZ2. Using a combination of gain- and loss-of-function approaches in several cellular contexts, we show that YAP/TAZ are necessary to induce the effects of the inactivation of the SWI/SNF complex, such as cell proliferation, acquisition of stem cell-like traits and liver tumorigenesis. We found that YAP/TAZ form a complex with SWI/SNF; this interaction is mediated by ARID1A and is alternative to the association of YAP/TAZ with the DNA-binding platform TEAD. Cellular mechanotransduction regulates the association between ARID1A-SWI/SNF and YAP/TAZ. The inhibitory interaction of ARID1A-SWI/SNF and YAP/TAZ is predominant in cells that experience low mechanical signalling, in which loss of ARID1A rescues the association between YAP/TAZ and TEAD. At high mechanical stress, nuclear F-actin binds to ARID1A-SWI/SNF, thereby preventing the formation of the ARID1A-SWI/SNF-YAP/TAZ complex, in favour of an association between TEAD and YAP/TAZ. We propose that a dual requirement must be met to fully enable the YAP/TAZ responses: promotion of nuclear accumulation of YAP/TAZ, for example, by loss of Hippo signalling, and inhibition of ARID1A-SWI/SNF, which can occur either through genetic inactivation or because of increased cell mechanics. This study offers a molecular framework in which mechanical signals that emerge at the tissue level together with genetic lesions activate YAP/TAZ to induce cell plasticity and tumorigenesis.


Subject(s)
Adaptor Proteins, Signal Transducing/antagonists & inhibitors , Adaptor Proteins, Signal Transducing/metabolism , DNA-Binding Proteins/metabolism , Mechanotransduction, Cellular , Multiprotein Complexes/metabolism , Nuclear Proteins/antagonists & inhibitors , Nuclear Proteins/metabolism , Transcription Factors/antagonists & inhibitors , Actins/metabolism , Adaptor Proteins, Signal Transducing/genetics , Animals , Carcinogenesis/genetics , Cell Cycle Proteins , Cell Line , Cell Nucleus/metabolism , Cell Proliferation , DNA-Binding Proteins/deficiency , DNA-Binding Proteins/genetics , Female , Hippo Signaling Pathway , Humans , Male , Mice , Multiprotein Complexes/chemistry , Multiprotein Complexes/deficiency , Multiprotein Complexes/genetics , Nuclear Proteins/genetics , Protein Binding , Protein Serine-Threonine Kinases/metabolism , Stress, Mechanical , TEA Domain Transcription Factors , Trans-Activators , Transcription Factors/metabolism , Wnt Signaling Pathway
9.
Nat Med ; 24(10): 1599-1610, 2018 10.
Article in English | MEDLINE | ID: mdl-30224758

ABSTRACT

Cancer cells rely on dysregulated gene expression. This establishes specific transcriptional addictions that may be therapeutically exploited. Yet, the mechanisms that are ultimately responsible for these addictions are poorly understood. Here, we investigated the transcriptional dependencies of transformed cells to the transcription factors YAP and TAZ. YAP/TAZ physically engage the general coactivator bromodomain-containing protein 4 (BRD4), dictating the genome-wide association of BRD4 to chromatin. YAP/TAZ flag a large set of enhancers with super-enhancer-like functional properties. YAP/TAZ-bound enhancers mediate the recruitment of BRD4 and RNA polymerase II at YAP/TAZ-regulated promoters, boosting the expression of a host of growth-regulating genes. Treatment with small-molecule inhibitors of BRD4 blunts YAP/TAZ pro-tumorigenic activity in several cell or tissue contexts, causes the regression of pre-established, YAP/TAZ-addicted neoplastic lesions and reverts drug resistance. This work sheds light on essential mediators, mechanisms and genome-wide regulatory elements that are responsible for transcriptional addiction in cancer and lays the groundwork for a rational use of BET inhibitors according to YAP/TAZ biology.


Subject(s)
Adaptor Proteins, Signal Transducing/genetics , Phosphoproteins/genetics , Transcription Factors/genetics , Transcription, Genetic , Triple Negative Breast Neoplasms/genetics , Acyltransferases , Carcinogenesis/drug effects , Cell Cycle Proteins , Cell Line, Tumor , Drug Resistance, Neoplasm/genetics , Female , HEK293 Cells , Humans , Nuclear Proteins/antagonists & inhibitors , RNA Polymerase II/genetics , Regulatory Elements, Transcriptional/drug effects , Small Molecule Libraries/pharmacology , Transcription Factors/antagonists & inhibitors , Triple Negative Breast Neoplasms/pathology , YAP-Signaling Proteins
10.
Nat Commun ; 8: 15206, 2017 05 17.
Article in English | MEDLINE | ID: mdl-28513598

ABSTRACT

How the behaviour of somatic stem cells (SCs) is influenced by mechanical signals remains a black-box in cell biology. Here we show that YAP/TAZ regulation by cell shape and rigidity of the extracellular matrix (ECM) dictates a pivotal SC decision: to remain undifferentiated and grow, or to activate a terminal differentiation programme. Notably, mechano-activation of YAP/TAZ promotes epidermal stemness by inhibition of Notch signalling, a key factor for epidermal differentiation. Conversely, YAP/TAZ inhibition by low mechanical forces induces Notch signalling and loss of SC traits. As such, mechano-dependent regulation of YAP/TAZ reflects into mechano-dependent regulation of Notch signalling. Mechanistically, at least in part, this is mediated by YAP/TAZ binding to distant enhancers activating the expression of Delta-like ligands, serving as 'in cis' inhibitors of Notch. Thus YAP/TAZ mechanotransduction integrates with cell-cell communication pathways for fine-grained orchestration of SC decisions.


Subject(s)
Adaptor Proteins, Signal Transducing/metabolism , Cell Lineage , Epidermal Cells , Intracellular Signaling Peptides and Proteins/metabolism , Phosphoproteins/metabolism , Receptors, Notch/metabolism , Stem Cells/cytology , Actins/metabolism , Animals , Cell Cycle Proteins , Cell Differentiation , Cell Shape , Epistasis, Genetic , Extracellular Matrix/metabolism , Humans , Infant, Newborn , Male , Mechanotransduction, Cellular , Mice, Transgenic , Models, Biological , Reproducibility of Results , Signal Transduction , Stem Cells/metabolism , Trans-Activators , Transcription Factors , Transcription, Genetic , Transcriptional Coactivator with PDZ-Binding Motif Proteins , YAP-Signaling Proteins
11.
Curr Opin Pharmacol ; 29: 26-33, 2016 08.
Article in English | MEDLINE | ID: mdl-27262779

ABSTRACT

The biology and regulation of YAP and TAZ, two closely related transcriptional regulators, are receiving increasing attention owing to their fundamental roles in organ growth, tissue repair and cancer. In particular, the widespread activation of YAP/TAZ in carcinomas, and the crucial role of YAP/TAZ activation for many 'hallmarks' of cancer are indicating YAP/TAZ as prime targets for designing anti-cancer drugs. Here, we start from the known modalities to regulate YAP/TAZ to highlight possible routes of therapeutic intervention.


Subject(s)
Adaptor Proteins, Signal Transducing/metabolism , Neoplasms/pathology , Phosphoproteins/metabolism , Transcription Factors/metabolism , Acyltransferases , Animals , Antineoplastic Agents/pharmacology , Drug Design , Humans , Molecular Targeted Therapy , Neoplasms/drug therapy , YAP-Signaling Proteins
12.
Nat Cell Biol ; 17(9): 1218-27, 2015 Sep.
Article in English | MEDLINE | ID: mdl-26258633

ABSTRACT

YAP/TAZ are nuclear effectors of the Hippo pathway regulating organ growth and tumorigenesis. Yet, their function as transcriptional regulators remains underinvestigated. By ChIP-seq analyses in breast cancer cells, we discovered that the YAP/TAZ transcriptional response is pervasively mediated by a dual element: TEAD factors, through which YAP/TAZ bind to DNA, co-occupying chromatin with activator protein-1 (AP-1, dimer of JUN and FOS proteins) at composite cis-regulatory elements harbouring both TEAD and AP-1 motifs. YAP/TAZ/TEAD and AP-1 form a complex that synergistically activates target genes directly involved in the control of S-phase entry and mitosis. This control occurs almost exclusively from distal enhancers that contact target promoters through chromatin looping. YAP/TAZ-induced oncogenic growth is strongly enhanced by gain of AP-1 and severely blunted by its loss. Conversely, AP-1-promoted skin tumorigenesis is prevented in YAP/TAZ conditional knockout mice. This work highlights a new layer of signalling integration, feeding on YAP/TAZ function at the chromatin level.


Subject(s)
Enhancer Elements, Genetic , Gene Expression Regulation, Neoplastic , Skin Neoplasms/genetics , Transcription Factor AP-1/genetics , Transcriptional Activation , Acyltransferases , Adaptor Proteins, Signal Transducing/physiology , Animals , Carcinogenesis/genetics , Carcinogenesis/metabolism , Cell Line, Tumor , DNA-Binding Proteins/physiology , Female , Genome-Wide Association Study , HEK293 Cells , Humans , Male , Mice, Knockout , Nuclear Proteins/physiology , Phosphoproteins/physiology , Protein Binding , Signal Transduction , Skin Neoplasms/metabolism , Skin Neoplasms/pathology , TEA Domain Transcription Factors , Transcription Factors/physiology , Tumor Burden , YAP-Signaling Proteins
13.
Dis Model Mech ; 7(7): 883-94, 2014 Jul.
Article in English | MEDLINE | ID: mdl-24878567

ABSTRACT

Pancreatic adenocarcinoma, one of the worst malignancies of the exocrine pancreas, is a solid tumor with increasing incidence and mortality in industrialized countries. This condition is usually driven by oncogenic KRAS point mutations and evolves into a highly aggressive metastatic carcinoma due to secondary gene mutations and unbalanced expression of genes involved in the specific signaling pathways. To examine in vivo the effects of KRAS(G12D) during pancreatic cancer progression and time correlation with cancer signaling pathway activities, we have generated a zebrafish model of pancreatic adenocarcinoma in which eGFP-KRAS(G12D) expression was specifically driven to the pancreatic tissue by using the GAL4/UAS conditional expression system. Outcrossing the inducible oncogenic KRAS(G12D) line with transgenic zebrafish reporters, harboring specific signaling responsive elements of transcriptional effectors, we were able to follow TGFß, Notch, Bmp and Shh activities during tumor development. Zebrafish transgenic lines expressing eGFP-KRAS(G12D) showed normal exocrine pancreas development until 3 weeks post fertilization (wpf). From 4 to 24 wpf we observed several degrees of acinar lesions, characterized by an increase in mesenchymal cells and mixed acinar/ductal features, followed by progressive bowel and liver infiltrations and, finally, highly aggressive carcinoma. Moreover, live imaging analysis of the exocrine pancreatic tissue revealed an increasing number of KRAS-positive cells and progressive activation of TGFß and Notch pathways. Increase in TGFß, following KRAS(G12D) activation, was confirmed in a concomitant model of medulloblastoma (MDB). Notch and Shh signaling activities during tumor onset were different between MDB and pancreatic adenocarcinoma, indicating a tissue-specific regulation of cell signaling pathways. Moreover, our results show that a living model of pancreatic adenocarcinoma joined with cell signaling reporters is a suitable tool for describing in vivo the signaling cascades and molecular mechanisms involved in tumor development and a potential platform to screen for novel oncostatic drugs.


Subject(s)
Genes, Reporter , Pancreatic Neoplasms/genetics , Pancreatic Neoplasms/pathology , Signal Transduction , Zebrafish/genetics , Adenocarcinoma/genetics , Adenocarcinoma/pathology , Animals , Animals, Genetically Modified , Apoptosis/genetics , Biomarkers/metabolism , Cell Proliferation , Cell Transformation, Neoplastic/genetics , Cell Transformation, Neoplastic/pathology , Disease Models, Animal , Disease Progression , Epithelial-Mesenchymal Transition/genetics , Gene Expression Regulation, Neoplastic , Green Fluorescent Proteins/metabolism , Kaplan-Meier Estimate , Medulloblastoma/pathology , Mutant Proteins/metabolism , Organ Specificity , Promoter Regions, Genetic/genetics , Proto-Oncogene Proteins p21(ras) , Transcription Factors/genetics , Zebrafish Proteins/metabolism
14.
J Neurooncol ; 116(3): 505-13, 2014 Feb.
Article in English | MEDLINE | ID: mdl-24401960

ABSTRACT

5-aminolevulinic acid (5-ALA) introduction in the surgical management of Glioblastoma (GBM) enables the intra-operatively identification of cancer cells in the mass by means of fluorescence. Here, we analyzed the phenotype of GBM cells isolated from distinct tumour areas determined by 5-ALA (tumour core, 5-ALA intense and vague layers) and the potency of 5-ALA labelling in identifying GBM cells and cancer stem cells (CSCs) in the mass. 5-ALA identified distinct layers in the mass, with less differentiated cells residing in the core of the tumour. 5-ALA was able to stain up to 68.5% of CD133(+) cells in the 5-ALA intense layer and, although 5-ALA(+) cells retrieved from different tumour areas contained a similar proportion of CD133(+) cells (range 27.5-35.6%), those from the vague layer displayed the lowest ability to self-renew. In conclusion, our data demonstrate that a substantial amount of GBM cells and CSCs in the mass are able to avoid 5-ALA labelling and support the presence of heterogenic CSC populations in the GBM mass.


Subject(s)
Aminolevulinic Acid , Brain Neoplasms/pathology , Glioblastoma/pathology , Neoplastic Stem Cells/pathology , Photosensitizing Agents , AC133 Antigen , Aminolevulinic Acid/metabolism , Antigens, CD/metabolism , Biopsy , Brain Neoplasms/surgery , Flow Cytometry , Glioblastoma/surgery , Glycoproteins/metabolism , Humans , Ki-67 Antigen/metabolism , Neoplastic Stem Cells/metabolism , Nerve Tissue Proteins/metabolism , Peptides/metabolism , Photosensitizing Agents/metabolism
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