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1.
Plant Physiol Biochem ; 151: 10-20, 2020 Jun.
Article in English | MEDLINE | ID: mdl-32179468

ABSTRACT

Soldanella alpina differing in leaf epidermal UV-A absorbance (DEA375), as measured with the Dualex, was investigated as a model alpine plant for the flavonoid (Flav) composition and concentration and for anatomical and pigment characteristics. In sun leaves, twenty-three flavones were characterised by their mass formula, their maximum absorption, their glycosylation, their methylation and dehydroxylation pattern. The flavones belonged to four subfamilies (tetra-hydroxy-flavones, penta-hydroxy-flavones, penta-hydroxy-methyl-flavones and tri-hydroxy-di-methoxy-flavones), abundant in sun and shade leaves. Their concentration was estimated by their absorption at 350 nm after HPLC separation. Sun leaves contained relatively higher contents of penta-hydroxy-methyl-flavones and shade leaves higher contents of tetra-hydroxy-flavones. The flavones were present mainly in vacuoles, all over the leaf. After shade-sun transfer, the content of most flavones increased, irrespective of the presence or absence of UV radiation. Highly significant correlations with the log-transformed DEA375 suggest that DEA375 can be readily applied to predict the flavone content of S. alpina leaves. Shade-sun transfer of leaves decreased the hydroxycinnamic acid (HCA) content, the mass-based chlorophyll (Chl) a+b content and the Chl/Carotenoid (Car) ratio but increased DEA375, and the Car content. Together with previously reported anatomical characteristics all these parameters correlated significantly with the DEA375. The Flav content is therefore correlated to most of the structural characteristics of leaf acclimation to light and this can be probed in situ by DEA375.


Subject(s)
Acclimatization , Plant Leaves/physiology , Primulaceae/physiology , Ultraviolet Rays , Carotenoids/analysis , Chlorophyll/analysis , Flavonoids/analysis , Photosynthesis , Plant Leaves/radiation effects , Primulaceae/radiation effects , Sunlight
2.
Physiol Plant ; 168(3): 563-575, 2020 Mar.
Article in English | MEDLINE | ID: mdl-31090072

ABSTRACT

In the French Alps, Soldanella alpina (S. alpina) grow under shade and sun conditions during the vegetation period. This species was investigated as a model for the dynamic acclimation of shade leaves to the sun under natural alpine conditions, in terms of photosynthesis and leaf anatomy. Photosynthetic activity in sun leaves was only slightly higher than in shade leaves. The leaf thickness, the stomatal density and the epidermal flavonoid content were markedly higher, and the chlorophyll/flavonoid ratio was significantly lower in sun than in shade leaves. Sun leaves also had a more oxidised plastoquinone pool, their PSII efficiency in light was higher and their non-photochemical quenching (NPQ) capacity was higher than that of shade leaves. Shade-sun transferred leaves increased their leaf thickness, stomatal density and epidermal flavonoid content, while their photosynthetic activity and chlorophyll/flavonoid ratio declined compared to shade leaves. Parameters indicating protection against high light and oxidative stress, such as NPQ and ascorbate peroxidase, increased in shade-sun transferred leaves and leaf mortality increased. We conclude that the dynamic acclimation of S. alpina leaves to high light under alpine conditions mainly concerns anatomical features and epidermal flavonoid acclimation, as well as an increase in antioxidative protection. However, this increase is not large enough to prevent damage under stress conditions and to replace damaged leaves.


Subject(s)
Acclimatization , Photosynthesis , Primulaceae/physiology , Sunlight , Chlorophyll , Oxidative Stress , Plant Leaves
3.
Development ; 146(14)2019 07 25.
Article in English | MEDLINE | ID: mdl-31239243

ABSTRACT

Bone morphogenetic proteins (BMPs) are secreted regulators of cell fate in several developing tissues. In the embryonic spinal cord, they control the emergence of the neural crest, roof plate and distinct subsets of dorsal interneurons. Although a gradient of BMP activity has been proposed to determine cell type identity in vivo, whether this is sufficient for pattern formation in vitro is unclear. Here, we demonstrate that exposure to BMP4 initiates distinct spatial dynamics of BMP signalling within the self-emerging epithelia of both mouse and human pluripotent stem cell-derived spinal organoids. The pattern of BMP signalling results in the stereotyped spatial arrangement of dorsal neural tube cell types, and concentration, timing and duration of BMP4 exposure modulate these patterns. Moreover, differences in the duration of competence time-windows between mouse and human account for the species-specific tempo of neural differentiation. Together, this study describes efficient methods for generating patterned subsets of dorsal interneurons in spinal organoids and supports the conclusion that graded BMP activity orchestrates the spatial organization of the dorsal neural tube cellular diversity in mouse and human.


Subject(s)
Bone Morphogenetic Protein 4/physiology , Cell Differentiation/genetics , Organoids/physiology , Smad Proteins/metabolism , Spine/cytology , Animals , Cell Lineage/genetics , Cells, Cultured , Embryo, Mammalian , Gene Expression Regulation, Developmental , Humans , Induced Pluripotent Stem Cells/cytology , Induced Pluripotent Stem Cells/physiology , Interneurons/cytology , Interneurons/physiology , Mice , Neural Crest/cytology , Neural Crest/physiology , Neural Tube/cytology , Neural Tube/embryology , Neurons/cytology , Neurons/physiology , Organoids/cytology , Signal Transduction/genetics , Smad Proteins/genetics
4.
Cell Host Microbe ; 18(5): 593-603, 2015 Nov 11.
Article in English | MEDLINE | ID: mdl-26607162

ABSTRACT

Plasmodium sporozoites are deposited in the host skin by Anopheles mosquitoes. The parasites migrate from the dermis to the liver, where they invade hepatocytes through a moving junction (MJ) to form a replicative parasitophorous vacuole (PV). Malaria sporozoites need to traverse cells during progression through host tissues, a process requiring parasite perforin-like protein 1 (PLP1). We find that sporozoites traverse cells inside transient vacuoles that precede PV formation. Sporozoites initially invade cells inside transient vacuoles by an active MJ-independent process that does not require vacuole membrane remodeling or release of parasite secretory organelles typically involved in invasion. Sporozoites use pH sensing and PLP1 to exit these vacuoles and avoid degradation by host lysosomes. Next, parasites enter the MJ-dependent PV, which has a different membrane composition, precluding lysosome fusion. The malaria parasite has thus evolved different strategies to evade host cell defense and establish an intracellular niche for replication.


Subject(s)
Malaria/pathology , Malaria/parasitology , Plasmodium berghei/metabolism , Plasmodium yoelii/metabolism , Sporozoites/pathology , Sporozoites/parasitology , Vacuoles/parasitology , Animals , Anopheles/parasitology , Hep G2 Cells , Hepatocytes/pathology , Hepatocytes/ultrastructure , Humans , Membrane Proteins/metabolism , Mice , Mice, Inbred BALB C , Plasmodium berghei/growth & development , Plasmodium berghei/ultrastructure , Plasmodium yoelii/growth & development , Plasmodium yoelii/ultrastructure , Protozoan Proteins/metabolism , Sporozoites/metabolism , Vacuoles/metabolism , Vacuoles/ultrastructure
5.
Mol Biol Cell ; 26(12): 2343-56, 2015 Jun 15.
Article in English | MEDLINE | ID: mdl-25904327

ABSTRACT

Nup98 is a glycine-leucine-phenylalanine-glycine (GLFG) repeat-containing nucleoporin that, in addition to nuclear transport, contributes to multiple aspects of gene regulation. Previous studies revealed its dynamic localization within intranuclear structures known as GLFG bodies. Here we show that the mammalian Nup107-160 complex (Y-complex), a major scaffold module of the nuclear pore, together with its partner Elys, colocalizes with Nup98 in GLFG bodies. The frequency and size of GLFG bodies vary among HeLa sublines, and we find that an increased level of Nup98 is associated with the presence of bodies. Recruitment of the Y-complex and Elys into GLFG bodies requires the C-terminal domain of Nup98. During cell division, Y-Nup-containing GLFG bodies are disassembled in mitotic prophase, significantly ahead of nuclear pore disassembly. FRAP studies revealed that, unlike at nuclear pores, the Y-complex shuttles into and out of GLFG bodies. Finally, we show that within the nucleoplasm, a fraction of Nup107, a key component of the Y-complex, displays reduced mobility, suggesting interaction with other nuclear components. Together our data uncover a previously neglected intranuclear pool of the Y-complex that may underscore a yet-uncharacterized function of these nucleoporins inside the nucleus, even in cells that contain no detectable GLFG bodies.


Subject(s)
Cell Nucleus/metabolism , Nuclear Pore Complex Proteins/metabolism , Nuclear Proteins/metabolism , Cell Nucleus/physiology , DNA-Binding Proteins/metabolism , Fluorescence Recovery After Photobleaching , HeLa Cells , Humans , Mitosis , Transcription Factors/metabolism
6.
Chromosome Res ; 23(2): 333-42, 2015 Jun.
Article in English | MEDLINE | ID: mdl-25772408

ABSTRACT

The presence or absence of Mad1 at kinetochores is a major determinant of spindle assembly checkpoint (SAC) activity, the surveillance mechanism that delays anaphase onset if one or more kinetochores remain unattached to spindle fibers. Among the factors regulating the levels of Mad1 at kinetochores is the Rod, Zw10, and Zwilch (RZZ) complex, which is required for Mad1 recruitment through a mechanism that remains unknown. The relative dynamics and interactions of Mad1 and RZZ at kinetochores have not been extensively investigated, although Mad1 has been reported to be stably recruited to unattached kinetochores. In this study, we directly compare Mad1-green fluorescent protein (GFP) turnover dynamics on unattached Drosophila kinetochores with that of RZZ, tagged either with GFP-Rod or GFP-Zw10. We find that nearly 40 % of kinetochore-bound Mad1 has a significant dynamic component, turning over with a half-life of 12 s. RZZ in contrast is essentially stable on unattached kinetochores. In addition, we report that a fraction of RZZ and Mad1 can co-immunoprecipitate, indicating that the genetically determined recruitment hierarchy (in which Mad1 depends on RZZ) may reflect a physical association of the two complexes.


Subject(s)
Drosophila Proteins/metabolism , Drosophila/genetics , Drosophila/metabolism , Kinetochores/metabolism , Animals , Animals, Genetically Modified , Gene Expression , Gene Order , Genes, Reporter , Genetic Loci , M Phase Cell Cycle Checkpoints , Protein Binding , Protein Transport , Spindle Apparatus/metabolism , Transgenes
7.
Funct Plant Biol ; 42(7): 599-608, 2015 Jun.
Article in English | MEDLINE | ID: mdl-32480704

ABSTRACT

Alpine plants like Soldanella alpina L. are subjected to high PAR and high UV radiation. Among the important photoprotective mechanisms that prevent photoinhibition under such conditions, passive optical barriers such as UV-absorbing compounds were investigated. In this study, temporal and spatial patterns of epidermal UV-A absorbance for S. alpina leaves were investigated with a combination of absorbance measurements at 375nm and imaging methods. UV-A absorbance was highest in plants acclimated to full sunlight and was markedly stable during the leaves' lifetime. UV-A absorbance was correlated with leaf structure (leaf mass per area ratio, density of epidermal cells and stomata) and biochemical features such as chlorophyll and carotenoid content and ratio, which are characteristics of light acclimation. UV-A-absorbing compounds were mainly localised in the epidermal vacuoles and trichomes. Leaves with low UV-A absorbance were significantly more photosensitive than leaves with high UV-A absorbance. However, the epidermal UV-A absorbance increased in low-absorbance leaves under full sunlight even in the absence of UV radiation. Results suggest that high epidermal UV-A absorbance protects S. alpina leaves from photoinactivation, which is especially important after snowmelt, when plants are suddenly exposed to full sunlight.

8.
J Pathol ; 234(4): 452-63, 2014 Dec.
Article in English | MEDLINE | ID: mdl-24979321

ABSTRACT

Cirrhosis is a lesion at risk of hepatocellular carcinoma (HCC). Identifying mechanisms associated with the transition from cirrhosis to HCC and characterizing biomarkers of cirrhosis at high risk of developing into cancer are crucial for improving early diagnosis and prognosis of HCC. We used MALDI imaging to compare mass spectra obtained from tissue sections of cirrhosis without HCC, cirrhosis with HCC, and HCC, and a top-down proteomics approach to characterize differential biomarkers. We identified a truncated form of monomeric ubiquitin lacking the two C-terminal glycine residues, Ubi(1-74), the level of which increased progressively, from cirrhosis without HCC to cirrhosis with HCC to HCC. We showed that kallikrein-related peptidase 6 (KLK6) catalysed the production of Ubi(1-74) from monomeric ubiquitin. Furthermore, we demonstrated that KLK6 was induced de novo in cirrhosis and increased in HCC in parallel with accumulation of Ubi(1-74). We investigated in vitro the possible consequences of Ubi(1-74) accumulation and demonstrated that Ubi(1-74) interferes with the normal ubiquitination machinery in what is likely to be a kinetic process. Our data suggest that de novo KLK6 expression during early liver carcinogenesis may induce production of Ubi(1-74) by post-translational modification of ubiquitin. Given the deleterious effect of Ubi(1-74) on protein ubiquitination and the major role of ubiquitin machinery in maintenance of cell homeostasis, Ubi(1-74) might severely impact a number of critical cellular functions during transition from cirrhosis to cancer. Ubi(1-74) and KLK6 may serve as markers of cancer risk in patients with cirrhosis.


Subject(s)
Carcinoma, Hepatocellular/pathology , Kallikreins/metabolism , Liver Cirrhosis/pathology , Liver Neoplasms/pathology , Ubiquitin/metabolism , Aged , Biomarkers, Tumor/metabolism , Blotting, Western , Carcinoma, Hepatocellular/metabolism , Disease Progression , Female , Humans , Immunohistochemistry , Liver Cirrhosis/metabolism , Liver Neoplasms/metabolism , Male , Middle Aged , Protein Processing, Post-Translational , Proteomics , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Support Vector Machine
9.
J Mol Biol ; 425(23): 4837-44, 2013 Nov 29.
Article in English | MEDLINE | ID: mdl-23810902

ABSTRACT

We analyzed formation of single-stranded DNA (ssDNA) related to SOS induction in nalidixilate (Nal)-treated Escherichia coli, using immunofluorescence microscopy accompanied by computer analysis. We found enhancement of both ssDNA concentrations and cells having ssDNA foci that often localized around cellpoles. Analyzing several mutants deficient in DNA repair or replication, we found, after Nal treatment, that recN, recA, uvrD and dnaB failed to increase ssDNA concentration and that recG and particularly ruvA only partially enhanced it. In Nal-treated recB mutant, despite its failure in SOS induction, ssDNA foci positive cells increased with a slight enhancement of its concentration. These observations suggest the existence of a cellular process that sequesters genotoxic ssDNA as inert form, offering a new concept for SOS suppressor genes action.


Subject(s)
DNA Replication , DNA, Bacterial/metabolism , DNA, Single-Stranded/metabolism , Escherichia coli/genetics , Escherichia coli/metabolism , Anti-Bacterial Agents/metabolism , DNA Repair Enzymes/genetics , DNA Repair Enzymes/metabolism , Escherichia coli/drug effects , Gene Knockout Techniques , Image Processing, Computer-Assisted , Microscopy, Fluorescence , Nalidixic Acid/metabolism , SOS Response, Genetics
10.
J Leukoc Biol ; 91(1): 83-95, 2012 Jan.
Article in English | MEDLINE | ID: mdl-21954286

ABSTRACT

Production of ROS by the leukocyte NADPH oxidase is essential for the destruction of pathogenic bacteria inside phagosomes. The enzyme is a complex of cytosolic and membranous subunits that need to assemble upon activation. Biochemical data suggest that the complex is renewed continuously during activity. Furthermore, it is generally assumed that complex assembly and activity occur in parallel. However, information about the oxidase assembly in individual phagosomes in live cells is scarce. We studied the dynamic behavior of the crucial cytosolic NADPH oxidase component p67(phox) during phagocytosis by videomicroscopy. p67(phox) is involved in the regulation of electron flow from NADPH to oxygen, leading to superoxide radical formation inside the phagosome. p67(phox)-citrine, expressed in myeloid PLB-985 cells, accumulated at the phagosomal membrane during phagocytosis of yeast particles. Using photobleaching techniques (FRAP, FLIP), we demonstrated that p67(phox)-citrine diffused freely in this phagosomal membrane, but the phagosomal pool of p67(phox)-citrine did not exchange with the cytosolic pool. This result suggests that once assembled in the NADPH oxidase complex, p67(phox) is stable in this complex. Furthermore, the time of the presence of p67(phox)-citrine at the phagosome increased substantially in the presence of complement in the opsonizing serum compared with decomplemented serum. PI(3)P also accumulated around phagosomes for twice as long in the presence of complement. The presence of p67(phox)-citrine was correlated with the duration of phagosomal ROS production in different opsonization conditions. These data support the critical role of p67(phox) for ROS production on the level of individual phagosomes.


Subject(s)
Neutrophils/metabolism , Phagocytosis/immunology , Phagosomes/metabolism , Phosphoproteins/metabolism , Reactive Oxygen Species/metabolism , Bacterial Proteins/genetics , Cell Differentiation/immunology , Cell Line, Tumor , Humans , Intracellular Membranes/immunology , Intracellular Membranes/metabolism , Leukemia, Myeloid , Luminescent Proteins/genetics , Neutrophils/cytology , Neutrophils/immunology , Phagosomes/immunology , Phosphoproteins/genetics
11.
Stem Cells ; 29(5): 812-24, 2011 May.
Article in English | MEDLINE | ID: mdl-21433223

ABSTRACT

Because stem cells are often found to improve repair tissue including heart without evidence of engraftment or differentiation, mechanisms underlying wound healing are still elusive. Several studies have reported that stem cells can fuse with cardiomyocytes either by permanent or partial cell fusion processes. However, the respective physiological impact of these two processes remains unknown in part because of the lack of knowledge of the resulting hybrid cells. To further characterize cell fusion, we cocultured mouse fully differentiated cardiomyocytes with human multipotent adipose-derived stem (hMADS) cells as a model of adult stem cells. We found that heterologous cell fusion promoted cardiomyocyte reprogramming back to a progenitor-like state. The resulting hybrid cells expressed early cardiac commitment and proliferation markers such as GATA-4, myocyte enhancer factor 2C, Nkx2.5, and Ki67 and exhibited a mouse genotype. Interestingly, human bone marrow-derived stem cells shared similar reprogramming properties than hMADS cells but not human fibroblasts, which suggests that these features might be common to multipotent cells. Furthermore, cardiac hybrid cells were preferentially generated by partial rather than permanent cell fusion and that intercellular structures composed of f-actin and microtubule filaments were involved in the process. Finally, we showed that stem cell mitochondria were transferred into cardiomyocytes, persisted in hybrids and were required for somatic cell reprogramming. In conclusion, by providing new insights into previously reported cell fusion processes, our data might contribute to a better understanding of stem cell-mediated regenerative mechanisms and thus, the development of more efficient stem cell-based heart therapies.


Subject(s)
Cell Fusion , Mesenchymal Stem Cells/cytology , Mitochondria/metabolism , Myocytes, Cardiac/cytology , Animals , Cell Differentiation/genetics , Cell Differentiation/physiology , Cell Line , Cells, Cultured , Cellular Reprogramming/genetics , Cellular Reprogramming/physiology , Fibroblasts/cytology , Fibroblasts/metabolism , Humans , Immunohistochemistry , In Situ Hybridization , Male , Mesenchymal Stem Cells/metabolism , Mice , Myocytes, Cardiac/metabolism , Reverse Transcriptase Polymerase Chain Reaction
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