Recruiting refugees and migrants as potential hematopoietic stem cell donors to serve patients of comparable ethnicities with rare human leucocyte antigen patterns - The BluStar.NRW project in North Western Germany.

Currently, approximately 19 million people with a migration background live in Germany. The majority of those descend from regions where the population has a genetically different distribution of HLA antigens when compared to the HLA frequencies usually found in North Western Europe. In case of severe haematological disorders of these individuals, allogeneic stem cell transplantation may be the treatment of choice. However, finding appropriate histocompatible hematopoietic stem cell donors continues to be a major challenge. If no matching sibling donors are available, there are only few suitable donors with a similar genetic background available in international blood stem cell donor registries. The "BluStar.NRW" project aimed to recruit new blood and hematopoietic stem cell donors with a migration background and to noticeably increase the number of suitable donors for patients within this group. Since December 2017, a total number of 9100 blood and stem cell donors with a migration background were recruited and typed for this project. HLA typing for HLA-A, -B, -C, -DRB1, -DQB1, and -DPB1 was performed by Next Generation Sequencing. We assessed the proportion of rare alleles according to HLA frequency tables, as defined by a frequency of <1:1000. The rare HLA allele frequencies according to HLA frequency tables of the BluStar.NRW cohort were compared with a matched control donor cohort: Rare HLA-A, -B, -C, -DRB1 and -DQB1 alleles occurred three times more frequent than in the control group, but rare HLA-DPB1 alleles occurred more frequently in the control cohort. This difference was highly significant for all HLA alleles (p < 0.0001 for HLA-A, -B, -C, -DRB1, -DPB1; p = 0.0002 for HLA-DQB1). In addition, the distribution of rare alleles differed between the two groups. To date, 29 work-ups were initiated, 12 PBSC, one BM and three DLI were collected so far out of the BluStar.NRW cohort. The apheresis probability is twofold higher (0.18% vs. 0.07%) compared to the control group which clearly shows a serious medical need. However, 13 work-ups were cancelled in the BluStar.NRW donor cohort which represents an almost twice as higher cancellation rate (45% vs. 25%). This single registry analysis with a large sample cohort clearly indicates that hematopoietic stem cell donors with a migration background represent an adequate donor pool to serve patients of comparable ethnicity.

Optical clearing of the eye using the See Deep Brain technique.

PurposeTissue clearing has been used in anatomy for the first time in Germany over a century ago. Neuronal tissue, like cortex, was investigated in mice using a water-based optical clearing method termed See Deep Brain (SeeDB). However, although the eye belongs to the central nervous system, this histological technique was not applied in the eye up to date. We applied SeeDB for the visualization of intraocular structures.Patients and methodsFour eyes of cornea donors (two male, two female: 73-84 years) obtained from the Cornea Bank of the Department of Ophthalmology Erlangen, four chicken eyes and two mices' optic nerve were used. Bulbi were fixed in 4% paraformaldehyde in phosphate-buffered saline and treated with increasing concentrations of aqueous fructose solution with 0.5% α-thioglycerol. After SeeDB, transscleral macrophotographs of the choroid were performed.ResultsComplete transparency of the sclera was obtained in enucleated human and chicken eyes after SeeDB treatment. Macroscopical anatomy of the choroid (partially transparent due to the remaining retinal pigment epithelium and melanocytes) showing vessels and other related structures was possible without preparing slides. Mice optic nerves were also transparent after SeeDB treatment.ConclusionThe SeeDB method allows visualization of intraocular structures through a completely translucent sclera. This innovative processing technique could facilitate comprehensive qualitative and quantitative topographical anatomical studies of human and animal eyes, preserving their 3D architecture. Supra- and intrachoroidal ganglionic plexus could potentially be visualized transsclerally. Finally, clinical-pathological correlations of intraocular diseases-for example, retinal tumors-will be possible in non-dissected eyes.

Match Running Performance and Success Across a Season in German Bundesliga Soccer Teams.

The aim of the study was to quantify the association between match running performance and success across a season in soccer teams competing within a European top league. We analyzed the match running performance data of all soccer teams from the German Bundesliga across the season 2012/13 (306 matches). The following match running performance data were used: total distance covered as well as number of running activities>18.0 km/h and > 22.7 km/h. Depending on the team's ball possession status, all match running performance data were also analyzed as those with and without ball possession. The success across the season was defined as the final competition points accumulated. The match running performance alone was not significantly correlated with the final points accumulated (best r=0.24; p=0.34). In contrast, positive-significant correlations were observed for the match running performance with ball possession (best r=0.77; p<0.01). However, of these latter correlations, only the total distance covered with ball possession was a significant predictor (p<0.01) and accounted for 60% of the variance (R(2)=0.60) in the final points accumulated. It is concluded that it is not the match running performance alone that is important for achieving success in German Bundesliga soccer teams, but rather its relation to technical/tactical skills with respect to ball possession.

[Reliability of Selected Parameters of Cycling Ergospirometry from the PowerCube-Ergo Respiratory Gas Analyser]. / Reliabilität ausgewählter Parameter der Fahrradergospirometrie anhand des PowerCube-Ergo-Atemgasanalysators.

This study aimed to investigate the reliability of 1) the key parameters of cycling ergospirometry (maximum power output [Pmax] and oxygen uptake [V̇O2peak], ventilatory thresholds 1 [VT 1] and 2 [VT 2], and cycling efficiency [CE] and gross efficiency [GE]), 2) the commonly used parameters to quantify exhaustion (maximum heart rate [HFmax], respiratory quotient [RQmax], blood lactate concentration [BLAmax], and ratings of perceived exhaustion [RPEmax]), and 3) the kinetics of exercise induced gas exchange measurements (oxygen uptake [V̇O2], carbon dioxide output [V̇CO2], and minute ventilation [V̇E]) using the PowerCube-Ergo metabolic system in consideration of international statistical recommendations. 12 women and 12 men (28 ±â€Š4 years; 23.2 ±â€Š2.4 kg/m(2)) performed two cycling tests (20 watt/min) separated by one week. The reliability was calculated based on differences in means (t test and effect sizes), retest correlation (intraclass correlation coefficient [ICC]), and within-subject variation (standard error of measurement [SEM]). Of the key parameters of cycling ergospirometry, an excellent reliability (ICC ≥ 0.969; p = 0.000) and high accuracy (%SEM ≤ 4.6) were found for Pmax, V̇O2peak, and VT 1. Of the most commonly used parameters to quantify exhaustion, an excellent reliability (ICC = 0.922; p = 0.000) and high accuracy (%SEM = 1.0) existed only for HFmax. The gas exchange measurements (V̇O2, V̇CO2 und V̇E) of the PowerCube-Ergo were all excellently reliable (ICC ≥ 0,991; p = 0.000) and the accuracy of V̇O2 (SEM = 0.10 l/min) and V̇E (SEM = 3.13 l/min) fulfilled the quality guidance of exercise physiology laboratories. For future studies and practical purposes, the results are vital for the decision as to whether a difference between two tests represents a true intervention effect or just a measurement error and for the estimation of required sample sizes.

Relationship between core strength and key variables of performance in elite rink hockey players.

AIM: The aim of this study was to test the hypothesis that a significant relationship exists between the level of core strength-endurance and key variables of endurance, strength, power, speed, and agility performance in male elite rink hockey players. METHODS: Ten male elite rink hockey players of the German national team were tested for 1) time to exhaustion, maximum oxygen uptake, and running economy, 2) one repetition maximum bench press and half squat, 3) counter movement jump height, 4) 5 m, 10 m, and 20 m speed, and 5) 22 m agility. The rink hockey players were also tested for 6) ventral, lateral-left, lateral-right, and dorsal core strength-endurance using concentric-eccentric muscle tests. RESULTS: The level of total and ventral core strength-endurance was very largely correlated with maximum oxygen uptake (r=0.74 and r=0.71, both P<0.05). Additionally, there was a large correlation between the level of ventral core strength-endurance and time to exhaustion (r=0.66, P<0.05). No further significant relationships were observed (best r=0.60, P>0.05). CONCLUSION: The findings from this study suggest that the level of core strength-endurance is largely to very largely correlated with key variables of endurance performance, but not significantly with strength, power, speed, or agility indicators in male elite rink hockey players. These findings should be noted by coaches and scientists when testing physical fitness or planning strength and conditioning programs for male elite rink hockey players.

Different endurance characteristics of female and male german soccer players.

The aims of the present study were to assess gender differences regarding lactate threshold and intermittent shuttle run performance in female and male soccer players as well as to investigate the relationships between both endurance characteristics in both genders. Fourteen female (1(st) division) and thirteen male (4(th) division) soccer players completed an incremental test (IT) to determine running velocities at 2 and 4 mmol · l(-1) blood lactate (v2 and v4) and maximum velocity (vmax) as well as an interval shuttle run test (ISRT) to determine running distance. Based on v2 and v4 and their percentages in relation to vmax, three intensity zones were calculated: a low lactate zone (v4). Female soccer players have a lower v4 (8.2%), vmax (11.3%) and ISRT distance (31.6%). No gender difference was found in v2. In contrast to males, ISRT distance correlates with vmax as well as with v2 and v4 in female soccer players. The intensity zones v4 differ between genders. The present study revealed that gender differences increase when the running performance is intermittent including change of directions. In both genders, different relationships between lactate threshold and intermittent shuttle run performance exist. During incremental testing, the running performances of female and male players reflect different distributions of aerobic and anaerobic metabolic pathways. The revealed gender differences should be considered for soccer endurance training.

[Motivation and psychological suitability for work in a pediatric intensive care unit]. / Zur Motivation und psychologischen Eignung für die Arbeit auf einer pädiatrischen Intensivstation.

During interviews with physicians and nurses on the general pediatric intensive care unit (ICU) of the University of Heidelberg Children's Hospital personnel reported on various aspects of their work. One complex of questions dealt with the motivation to work in intensive medicine. Physicians and nurses showed different patterns of motivation. Physicians could be clearly divided into those who found intensive medicine fascinating and those who tended to fear it. Nurses' motivation is multifaceted and differentiated. The information gained from the interviews questions the sense of obligating physicians without interest or aptitude to rotate to an ICU during their specialty training in pediatrics. This policy is associated with corresponding risks for patients. The work of often superiorly qualified nurses becomes more difficult.

Degradation of unsaturated fatty acids in peroxisomes. Existence of a 2,4-dienoyl-CoA reductase pathway.

1. There are two 2,4-dienoyl-CoA reductases (EC 1.3.1.-) (formerly called 4-enoyl-CoA reductase, Eur. J. Biochem. (1978) 91, 533-544) in rat liver, one in mitochondria and another one in peroxisomes. 2. Treatment of rats with clofibrate increases the activities of the 2,4-dienoyl-CoA reductases in both cell organelles. 3. Isolated peroxisomes metabolize 4-cis-decenoyl-CoA, a metabolite of linoleic acid, via the 2,4-dienoyl-CoA reductase pathway. 4. From these results it is concluded that peroxisomes have the enzymatic apparatus necessary for the degradation of unsaturated fatty acids.