ABSTRACT
We report on the use of diffusion measurements to gauge the fluidity and surface binding properties of a molecular monolayer. The monolayer film consists of octadecyl-1-phosphonic acid (ODPA) and controlled amounts of a lyso-phosphatidic acid tagged with the fluorescent probe BODIPY (BLPA). The monolayer films were formed using a Langmuir-Blodgett (LB) trough and deposited onto a glass slide. Monolayer morphology was characterized during film formation using Brewster angle microscopy (BAM). Fluorescence Recovery After Photobleaching (FRAP) microscopy was used to measure translational diffusion of BLPA and Fluorescence Anisotropy Decay Imaging (FADI) was used to measure rotational diffusion of the BLPA chromophore. These results provide information on the motional freedom of the probe and, importantly, on the strength of interaction between the probe and the support. Compositional variations in the monolayer give rise to changes in constituent dynamics that reflect intermolecular interactions.
ABSTRACT
We report on the formation of a chemically bound 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) monolayer on modified Au and silica surfaces, and changes in the organization of the interfacial lipid layer associated with immersion in aqueous solution. We have studied the interface using steady state and time resolved fluorescence spectroscopy, water contact angle and optical ellipsometry measurements, and electrochemical methods. Our data reveal that the DMPC adlayer in contact with air forms a relatively well organized interface that mediates the rotational motion of perylene. In the presence of an aqueous overlayer, perylene reorientation becomes more rapid, consistent with a reduction in the organization of the interfacial lipid adlayer. One implication of this finding is that the interfacial adlayer is less than a uniform monolayer, which is confirmed by electrochemical data. Our data underscore the importance of water in mediating the organization of interfacial lipid adlayers.
