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1.
J BUON ; 14(3): 511-4, 2009.
Article in English | MEDLINE | ID: mdl-19810147

ABSTRACT

Coexistence of renal cell carcinoma (RCC) and multiple myeloma (MM) is an extremely rare condition. Appearance of synchronous RCC and MM was not reported independently so far. In this brief communication, we report 2 cases of synchronous RCC and MM, discuss common risk factors or pathogenetic mechanisms seen in either RCC or MM, point out the importance of IL-6 in this coexistence and provide some descriptive properties of all reported synchronous RCC and MM cases.


Subject(s)
Carcinoma, Renal Cell/diagnosis , Interleukin-6/metabolism , Kidney Neoplasms/diagnosis , Multiple Myeloma/diagnosis , Neoplasms, Multiple Primary/diagnosis , Carcinoma, Renal Cell/epidemiology , Carcinoma, Renal Cell/therapy , Humans , Interleukin-6/blood , Kidney Neoplasms/epidemiology , Kidney Neoplasms/therapy , Male , Middle Aged , Multiple Myeloma/epidemiology , Multiple Myeloma/therapy , Neoplasms, Multiple Primary/epidemiology , Neoplasms, Multiple Primary/therapy , Risk Factors
2.
J Int Med Res ; 37(4): 1018-28, 2009.
Article in English | MEDLINE | ID: mdl-19761684

ABSTRACT

The renin-angiotensin system (RAS) is involved in cell growth, proliferation and differentiation in bone marrow in an autocrine-paracrine manner, and it modulates normal and neoplastic haematopoietic cell proliferation. This study aimed to assess expressions of the RAS components, renin, angiotensinogen and angiotensin-converting enzyme (ACE), during imatinib mesylate treatment of patients with chronic myeloid leukaemia (CML). Expressions of RAS components were studied in patients with CML at the time of diagnosis (n = 83) and at 3, 6 and 12 months after diagnosis (n = 35) by quantitative real-time polymerase chain reaction. De novo CML patients had increased ACE, angiotensinogen and renin mRNA levels and these expression levels decreased following administration of imatinib. The RAS activities were significantly different among Sokal risk groups of CML, highlighting the altered biological activity of RAS in neoplastic disorders. The results of this study confirm that haematopoietic RAS affects neoplastic cell production, which may be altered via administration of tyrosine kinase inhibitors such as imatinib mesylate.


Subject(s)
Leukemia, Myelogenous, Chronic, BCR-ABL Positive/drug therapy , Piperazines/pharmacology , Protein Kinase Inhibitors/pharmacology , Pyrimidines/pharmacology , Renin-Angiotensin System/drug effects , Adolescent , Adult , Aged , Aged, 80 and over , Angiotensinogen/genetics , Angiotensinogen/metabolism , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Benzamides , Bone Marrow/drug effects , Bone Marrow/pathology , Drug Therapy, Combination , Female , Gene Expression , Humans , Imatinib Mesylate , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/metabolism , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/physiopathology , Male , Middle Aged , Peptidyl-Dipeptidase A/genetics , Peptidyl-Dipeptidase A/metabolism , Piperazines/therapeutic use , Protein Kinase Inhibitors/therapeutic use , Pyrimidines/therapeutic use , Renin/genetics , Renin/metabolism , Renin-Angiotensin System/physiology , Young Adult
3.
Physiol Meas ; 27(7): 623-35, 2006 Jul.
Article in English | MEDLINE | ID: mdl-16705260

ABSTRACT

Chemical alterations of red blood cells (RBCs) during storage eventually affect the electrical properties of blood. In this study, the physiological parameters such as extracellular (SAGM + CPD + residual plasma) Na(+), K(+), Cl(-), pH, 2,3-DPG and ATP together with the Cole-Cole parameters were measured using erythrocyte suspensions from 51 male donors (31 donors form the training set and 20 donors are used for testing), on the 0th, 10th, 21st, 35th and 42nd days of storage. During storage, while the surrounding fluid resistance (R(e)) and the effective cell membrane capacitance (C(m)) increased progressively with time, the intracellular fluid resistance (R(i)) has decreased. Storage of RBCs resulted in a rise in K(+) and a fall in Na(+), Cl(-), pH, 2,3-DPG and ATP. Accordingly, electrical parameters were all correlated with Na(+), K(+), Cl(-), pH and ATP at varying levels. By applying multi-regression analysis, it is concluded that R(i), R(e) and C(m) are appropriate for modeling Na(+), K(+), Cl(-), pH and ATP during storage.


Subject(s)
Erythrocytes/metabolism , 2,3-Diphosphoglycerate/blood , Adenosine Triphosphate/blood , Blood Chemical Analysis/methods , Blood Chemical Analysis/statistics & numerical data , Blood Preservation , Chlorides/blood , Electric Impedance , Humans , Hydrogen-Ion Concentration , In Vitro Techniques , Male , Potassium/blood , Regression Analysis , Sodium/blood , Time Factors
4.
Transfus Med ; 15(2): 93-7, 2005 Apr.
Article in English | MEDLINE | ID: mdl-15859974

ABSTRACT

This observational study attempted to identify the effect of a natural disaster on the safety of blood supply and donor types with the influx of donors after a severe earthquake. Blood donation rate, blood discard rate and safety of blood donations responding to the earthquake, as projected from the infectious disease marker rate, were evaluated in blood donated immediately before (1 July-17 August) and after 17 August 1999 (17 August-21 August). These were compared with the results from the corresponding periods in 1998 and 2000 for donations at a university medical centre and two regional blood centres. 8055 units of allogeneic blood were collected at two regional blood centres, and 450 units were collected at a university medical centre during 4 days. Viral marker rates were nearly the same at the former but were slightly lower at the latter. The blood discard rate was nearly twice the comparative periods at the former, but it remained unchanged at the latter. Voluntary donors replaced the replacement donors during 4 days. This analysis highlights the size of the pool of potential donors that are available as a national resource that can be motivated to give blood with the right motivation.


Subject(s)
Blood Donors , Blood Transfusion , Blood-Borne Pathogens , Disasters , Virus Diseases , Biomarkers/blood , Blood Donors/psychology , Blood Donors/supply & distribution , Blood Transfusion/psychology , Humans , Virus Diseases/blood , Virus Diseases/prevention & control
7.
Clin Rheumatol ; 21(3): 211-4, 2002 Jun.
Article in English | MEDLINE | ID: mdl-12111626

ABSTRACT

Alpha-interferon (alpha-IFN) is implicated in a Behçet's disease (BD)-like syndrome observed in a small number of chronic myeloid leukemia (CML) patients. The effect of alpha-IFN on neutrophil adhesion and phagocytosis in CML patients, BD patients and healthy volunteers was investigated to clarify the reason for this observation. Ten subjects were studied for each group by incubating neutrophils with various doses of alpha-IFN. Basal neutrophil adhesions for CML patients, BD patients and healthy volunteers were similar. However, BD patients had greater basal phagocytosis than CML patients, and both groups had greater basal phagocytosis than healthy volunteers. Neutrophil adhesion and phagocytosis of CML patients increased following incubation with higher doses of alpha-IFN, and phagocytosis approached the high levels observed with BD neutrophils. This study provides evidence that alpha-IFN activates neutrophils in CML patients in a dose-dependent manner, and leads to a neutrophil function profile that resembles BD.


Subject(s)
Antineoplastic Agents/adverse effects , Behcet Syndrome/blood , Interferon-alpha/adverse effects , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/blood , Neutrophils/physiology , Phagocytosis/drug effects , Adult , Cell Adhesion/drug effects , Dose-Response Relationship, Drug , Female , Humans , Interferon alpha-2 , Male , Middle Aged , Recombinant Proteins , Reference Values
9.
Haematologica ; 85(5): 464-9, 2000 May.
Article in English | MEDLINE | ID: mdl-10800160

ABSTRACT

BACKGROUND AND OBJECTIVE: Hepatocyte growth factor (HGF) is known to augment the effects of stem cell factor, interleukin-3, granulocyte-macrophage colony-stimulating factor (GM-CSF), erythropoetin, and granulocyte colony-stimulating factor, all of which are involved in hematopoiesis. HGF is also known to have a role in immune responses. The aim of this study was to investigate whether HGF is involved in the development of dendritic cells (DC) from CD34+ bone marrow cells. DESIGN AND METHODS: CD34+ cells obtained from three healthy donors were incubated in various combinations of HGF, GM-CSF, and tumor necrosis factor (TNF) for 12 days. Developing cell populations were analyzed for surface markers, morphology and functional capacities by flow cytometry, light microscopy and mixed lymphocyte reaction, respectively. RESULTS: Incubation with HGF alone generated greater number of dendritic cells from CD34+ bone marrow cells than incubation with GM-CSF, or a combination of GM-CSF with TNF. HGF was also found to potentiate the effect of GM-CSF on DC and monocyte development. The effects of HGF were inhibited by the concurrent use of TNF. INTERPRETATION AND CONCLUSIONS: HGF appears to be a significant factor in the development of dendritic cells from CD34+ bone marrow cells.


Subject(s)
Antigens, CD34/analysis , Bone Marrow Cells/cytology , Bone Marrow Cells/immunology , Cell Differentiation/drug effects , Dendritic Cells/cytology , Hepatocyte Growth Factor/physiology , Antigens, CD/metabolism , Bone Marrow Cells/drug effects , Flow Cytometry , Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology , Hepatocyte Growth Factor/pharmacology , Humans , Lymphocyte Culture Test, Mixed , Time Factors , Tumor Necrosis Factor-alpha/pharmacology
10.
Turk J Haematol ; 17(1): 33-5, 2000 Mar 05.
Article in English | MEDLINE | ID: mdl-27265762

ABSTRACT

Chronic a-interferon use has been reported to cause a variety of neurotoxic side effects. This case summary suggests the possibility of a new neurotoxic side effect of normal pressure hydrocephalus following chronic a-interferon use.

11.
Leuk Lymphoma ; 33(5-6): 567-72, 1999 May.
Article in English | MEDLINE | ID: mdl-10342584

ABSTRACT

In order to investigate the relationship between aberrant marker expression and DNA ploidy, 61 adult patients with acute leukemia (39 AML and 22 ALL) were studied. Aberrant marker expression was observed in 20 patients (16/39 of AML and 4/22 of ALL patients). In flow cytometric DNA analysis aneuploidy was observed in 18 patients (9/39 of AML and 9/22 of ALL patients). The incidence of aneuploidy in patients with aberrant marker expression was 35% whereas this was 26.8% in patients without aberrant marker expression. Furthermore, 7 patients with aberrant marker expression showed an aneuploid, double G0/G1 peaks appearance whereas the remaining 11 patients with aberrant marker expression had euploid DNA content. Double G0/G1 appearance was not observed in patients without aberrant marker expression. Further analyses revealed that this did not correlate with apoptosis. All 7 patients, who had both aberrant marker expression and double G0/G1 peak had a poor clinical outcome with a short survival and all died within three months whereas three-months survival was 67% for AML, 69% for ALL patients and 81% for patients with aberrant marker expression respectively (p<0.01). Our data indicate that the evaluation of the DNA ploidy in patients with aberrant marker expression may be of prognostic importance.


Subject(s)
Aneuploidy , Biomarkers, Tumor , DNA, Neoplasm/genetics , Leukemia/genetics , Acute Disease , Adolescent , Adult , G1 Phase , Humans , Leukemia/pathology , Leukemia/physiopathology , Middle Aged , Predictive Value of Tests , Prognosis , Resting Phase, Cell Cycle
13.
Br J Haematol ; 103(4): 1061-3, 1998 Dec.
Article in English | MEDLINE | ID: mdl-9886319

ABSTRACT

Fifty-seven adult patients with idiopathic thrombocytopenic purpura (ITP) were treated with either conventional-dose prednisolone (CDP) (1 mg/kg/d, 36 patients) or high-dose methylprednisolone (HDP) (30 mg/kg/d, 21 patients), as first-line treatment. Patients in the HDP arm responded more rapidly (4.7 v 8.4 d), with a higher response rate (80% v 52.7%), and without severe side-effects. One quarter of the patients (3/12) who were non-responsive to CDP achieved complete remission when they were treated with HDP. The findings suggest that HDP may be a more effective first-line treatment than CDP for adult ITP, and it may also be preferred for life-threatening cases of ITP. However, these results must be confirmed by a randomized study prior to any change in the current practice of employing CDP as first-line treatment for adult ITP.


Subject(s)
Glucocorticoids/administration & dosage , Methylprednisolone/administration & dosage , Purpura, Thrombocytopenic, Idiopathic/drug therapy , Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Prednisolone/therapeutic use
14.
Spinal Cord ; 35(10): 704-7, 1997 Oct.
Article in English | MEDLINE | ID: mdl-9347602

ABSTRACT

Hodgkin's disease very rarely presents clinically, initially with a paraspinal mass, but this should be considered in the differential diagnosis. A patient presenting with back pain was diagnosed to have Stage IV Hodgkin's disease. MRI revealed an extradural and intraspinal soft tissue mass with bone infiltration. The importance of MRI in the early evaluation of a paraspinal mass and in determining the extent of the disease is emphasized.


Subject(s)
Cauda Equina , Epidural Neoplasms/diagnosis , Hodgkin Disease/diagnosis , Low Back Pain/etiology , Nerve Compression Syndromes/diagnosis , Adult , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Diagnosis, Differential , Hodgkin Disease/drug therapy , Hodgkin Disease/pathology , Humans , Magnetic Resonance Imaging , Male , Myelography , Neoplasm Staging
15.
Int J Dermatol ; 36(8): 593-8, 1997 Aug.
Article in English | MEDLINE | ID: mdl-9329890

ABSTRACT

BACKGROUND: Chemotherapy-induced acral erythema is a distinct localized cutaneous response to certain systemic chemotherapeutic agents. METHODS: Between January 1990 and December 1994, from a total of 76 leukemic patients who have received combination chemotherapy consisting of cytosine arabinoside and anthracycline antibiotics, 15 patients developed chemotherapy-induced acral erythema. Fourteen of the patients had acute myelocytic leukemia, and one of them had chronic myelogenous leukemia in blast phase. Clinical features of these 15 patients have been analysed. Biopsy specimens obtained from eight of the patients were also evaluated for histopathologic alterations. RESULTS: The overall incidence of this reaction was found to be 19.7% in our group of patients receiving this chemotherapy protocol. The onset of reaction varied from the fourth to the seventeenth days of the chemotherapy and resolved within 2 weeks in most of the patients. Lesions appeared as well-defined erythema and edema involving the palmar surfaces in all of the patients. In nine of the patients the reaction recurred with subsequent chemotherapies. Scattered necrotic keratinocytes, vacuolar alterations of the basal layer, and mild to moderate perivascular lymphocytic infiltration in the dermis were the histopathologic findings observed in the biopsy specimens. CONCLUSIONS: Chemotherapy-induced acral erythema is a frequent reaction in patients who are receiving high-dose chemotherapy. For patients in whom this self-limited condition develops, reassurance is the mainstay of therapy. Awareness of this reaction is also important to be able to differentiate it from acute graft versus host disease in patients who receive bone marrow transplants.


Subject(s)
Antineoplastic Combined Chemotherapy Protocols/adverse effects , Erythema/chemically induced , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/drug therapy , Leukemia, Myeloid, Acute/drug therapy , Adolescent , Adult , Aged , Antibiotics, Antineoplastic/adverse effects , Antibiotics, Antineoplastic/therapeutic use , Antimetabolites, Antineoplastic/adverse effects , Antimetabolites, Antineoplastic/therapeutic use , Cytarabine/adverse effects , Cytarabine/therapeutic use , Daunorubicin/adverse effects , Daunorubicin/therapeutic use , Doxorubicin/adverse effects , Doxorubicin/therapeutic use , Erythema/pathology , Female , Humans , Immunosuppressive Agents/adverse effects , Immunosuppressive Agents/therapeutic use , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/complications , Leukemia, Myeloid, Acute/complications , Male , Middle Aged , Prospective Studies , Recurrence
17.
Ann Hematol ; 74(1): 45-8, 1997 Jan.
Article in English | MEDLINE | ID: mdl-9031616

ABSTRACT

Two patients with chronic myelogenous leukemia (CML) who developed characteristic features of Behçet's disease (BD) during alpha-interferon (IFN-alpha) treatment and another patient who had a diagnosis of BD preceding CML are presented. In the first two patients, features of BD appeared 6 months after the initiation of IFN-alpha treatment: they included recurrent oral aphthae, genital ulceration, arthritis, folliculitis, and a positive skin pathergy test. The third patient, however, had a diagnosis of Behçet's disease 4 years before diagnosis of Philadelphia-positive CML. We prospectively examined the skin pathergy reaction in a group of patients with CML, multiple myeloma, and hairy cell leukemia both before and after IFN-alpha treatment and found two additional patients with CML who developed a positive skin pathergy test following IFN-alpha treatment.


Subject(s)
Behcet Syndrome/chemically induced , Behcet Syndrome/complications , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/complications , Adult , Female , Humans , Interferon-alpha/physiology , Male , Middle Aged
18.
Leukemia ; 10(8): 1331-9, 1996 Aug.
Article in English | MEDLINE | ID: mdl-8709639

ABSTRACT

We have investigated the expression of the dual specific adhesion molecule, VLA-4 (CD49d/CD29) on lymphocytes obtained from 62 patients with B-CLL and compared it with normal controls, patients with other hematological malignancies, and umbilical cord blood. The mean CD49d expression in patients with CLL was lower than the other group of leukemia and the CD19+, CD5+ cells of normal peripheral blood and umbilical cord blood (P < 0.001). The patients in RAI stage 0, I and II (early stage) had even lower CD49d expression, whereas patients in RAI stage III and IV (advanced stage) had relatively higher CD49d levels. In vitro adhesion of lymphocytes to fibronectin, being the extracellular matrix ligand of CD49d, was also investigated. Lymphocytes obtained from B-CLL were found to have lower adhesion to fibronectin than that from controls (P < 0.03). Furthermore, CD49d(low) B-CLL cells had lower adhesion to fibronectin, whereas CD49d(high) B-CLL cells showed normal adhesion ratios (P < 0.002). Further phenotypic analyses revealed the presence of myeloid markers (CD13 and CD33) in most of the advanced stage patients, although these were negative in early stage cases. Expressions of CD11a and sIgM were also low but CD11b was relatively higher in the early stages of the disease. On the basis of these results, we concluded that early stages of CLL are correlated with the expression of CD49d(low), CD11a(low), CD11b(high), CD13-, CD33-, sIgM(low) and also had lower fibronectin adhesion, whereas advanced stages of CLL are associated with CD49d(high), CD11a(high), CD11b(low), CD13+, CD33+, SIgM(high) and show normal fibronectin adhesion.


Subject(s)
Antigens, CD/blood , Leukemia, Lymphocytic, Chronic, B-Cell/blood , Leukemia, Lymphocytic, Chronic, B-Cell/immunology , Antibodies, Monoclonal , Bone Marrow/pathology , Female , Fetal Blood/immunology , Fibronectins/blood , Flow Cytometry , Humans , Immunophenotyping , Infant, Newborn , Integrin alpha4 , Leukemia, Lymphocytic, Chronic, B-Cell/pathology , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/blood , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/immunology , Leukemia, Myeloid, Acute/blood , Leukemia, Myeloid, Acute/immunology , Lymphoma, Non-Hodgkin/immunology , Lymphoma, Non-Hodgkin/pathology , Male , Neoplasm Staging , Precursor Cell Lymphoblastic Leukemia-Lymphoma/blood , Precursor Cell Lymphoblastic Leukemia-Lymphoma/immunology , Reference Values
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