ABSTRACT
Our earlier studies have shown that the flavonoid hesperidin inhibits tumor promotion in a two stage skin tumorigenesis protocol in CD-1 mice. In this study hesperidin's significant protection (p < 0.0001) against 12-0-tetradecanoylphorbol-13-acetate (TPA) induced edema in CD-1 mouse ear will be presented. Hesperidin also afforded significant protection against TPA induced hyperplasia in the dorsal skin through multiple application prior to TPA. Protection by hesperidin was 93% of the epidermal thickness and 100% of the vertical cell layers. The data also indicate the protective effect of hesperidin against TPA caused infiltration of neutrophils by 73%. These results suggest that hesperidin may possess potential as chemopreventive agent against tumor promoter induced inflammation and hyperplasia.
Subject(s)
Carcinogens/toxicity , Dermatitis, Contact/etiology , Hesperidin/pharmacology , Skin/drug effects , Animals , Female , Mice , Skin/pathologyABSTRACT
A flavonoid, Hesperidin was evaluated for its ability to inhibit tumour initiation by a polycyclic aromatic hydrocarbon and tumour promotion by a phorbol ester in the skin of CD-1 mice. Subcutaneous application of Hesperidin did not inhibit 7,12-dimethylbenz(a)anthracene-induced tumour initiation but did inhibit 12-O-tetradecanoyl-13-phorbol acetate-induced tumour promotion. Results provide evidence for a potential chemopreventive activity of Hesperidin.
Subject(s)
9,10-Dimethyl-1,2-benzanthracene , Carcinogens , Hesperidin/administration & dosage , Neoplasms, Experimental/prevention & control , Skin Neoplasms/prevention & control , Tetradecanoylphorbol Acetate , Administration, Cutaneous , Animals , Antineoplastic Agents/administration & dosage , Drug Antagonism , Female , Mice , Neoplasms, Experimental/chemically induced , Skin Neoplasms/chemically inducedABSTRACT
Of the chloro-substituted benzoic acids, the 2-chloro and 2,4-dichloro compounds yield higher effective molar absorptivities than benzoic acid does in the Rhodamine B-benzene extractive spectrophotometric procedure for determination of uranium(VI). Carbonyl compounds (especially acetone) in the organic phase enhance the extraction of the ion associate. The stoichiometry of the complexes has been determined, and a method of computing the extraction constants of the ion-associates developed. A spectrophotometric method for determining uranium in the presence of interfering ions has been designed.
