Effect of different litter materials on growth performance, the gait score and footpad dermatitis, carcass parameters, meat quality, and microbial load of litter in broiler chickens.

In the current study, the microbial load of litter materials, growth performance, the gait score and footpad dermatitis, carcass characteristics, and meat quality of broilers reared on different litter materials were investigated. After hatching and sex determination, the chicks were allocated to 3 different experimental groups with each having 8 replications. The chicks were reared on thick sawdust, fine sawdust, or rice hull litter materials. A total of 480 chicks were used with each replicate having 20 chicks (10 males, 10 females) of similar body weight. At the end of the experiment, a total of 96 chickens were slaughtered, 32 from each group with equal number of males and females. The effect of the experimental groups on body weight, mortality, and carcass characteristics were not significant; however, feed consumption and feed conversion ratio were significantly (P < 0.05) affected by the treatments in all weeks except the first 2 wk of the experiment. Different litter materials significantly (P < 0.05) influenced the foot health of the chickens as well as the microbial load of the litter. There was no significant difference between the treatment groups in terms of pH, color, and cutting resistance of raw meat however, the water loss during the cooking of the meat and the TPA analysis results such as hardness, springiness, and chewiness of the cooked meats were significantly (P < 0.05) affected by the type of litter material. It was concluded that the use of fine sawdust from trees such as pine and larch containing antimicrobial extracts would be more appropriate as a litter material in broiler production.

Variations in Multiple Syndromic Deafness Genes Mimic Non-syndromic Hearing Loss.

The genetics of both syndromic (SHL) and non-syndromic hearing loss (NSHL) is characterized by a high degree of genetic heterogeneity. We analyzed whole exome sequencing data of 102 unrelated probands with apparently NSHL without a causative variant in known NSHL genes. We detected five causative variants in different SHL genes (SOX10, MITF, PTPN11, CHD7, and KMT2D) in five (4.9%) probands. Clinical re-evaluation of these probands shows that some of them have subtle syndromic findings, while none of them meets clinical criteria for the diagnosis of the associated syndrome (Waardenburg (SOX10 and MITF), Kallmann (CHD7 and SOX10), Noonan/LEOPARD (PTPN11), CHARGE (CHD7), or Kabuki (KMT2D). This study demonstrates that individuals who are evaluated for NSHL can have pathogenic variants in SHL genes that are not usually considered for etiologic studies.

The need for mentoplasty following rhinoplasty.

OBJECTIVE: Rhinoplasty is the most common facial plastic surgery procedure. The appearance of the face must be considered as a whole to achieve the best results, but assessment of the chin is often overlooked. The aim of this paper was to determine the need for mentoplasty after rhinoplasty. METHODS: Adobe Photoshop CS6 (Adobe Systems, San Jose, CA) was used to analyze 200 consecutive rhinoplasty patients, 170 of whom were included in this study. We used Silver and Agarwal's method for assessment of the chin. RESULTS: A total of 170 profile photographs were analyzed. Of these patients, 58.2% (n = 99) had normal chins, 40% (n = 68) had small chins (microgenia), and 1.8% (n = 3) had over-projection. There were no statistically significant differences between men and women. CONCLUSION: Chin projection is an important component of facial euphony. Approximately half of the rhinoplasty patients we assessed would also benefit from mentoplasty. Surgeons that perform rhinoplasty should discuss mentoplasty with their patients to achieve the best results.

Bile acid conjugation in fetal hepatic organ cultures.

A new technique has been developed in which mammalian fetal liver can be maintained in organ culture for prolonged periods with intact structure and function. Near-term rat fetal liver explants were incubated in vitro for periods of up to 3 wk with preservation of normal cellular morphology and intercellular (organ) relationships. [14C]cholate was incorporated into tissue and medium conjugates at a constant rate during 21 days in vitro. During a 24-h incubation with radioactively labeled cholic acid, bile acid conjugates accumulated in tissues to a maximum value by 6 h and maintained this value through 24 h. During the same 24-h incubation with [14C]cholate, conjugates were secreted into the medium at a constant rate. Addition of 8 X 10(-4) M taurine to the medium during a 4-day incubation produced a threefold enhancement in the rate of conjugate formation in tissues and medium. Enhanced conjugation in the presence of additional taurine was due almost entirely to increased taurocholate formation and no significant difference was observed in the amount of glycocholate formed. Exposure of explants to 3.6 X 10(-4) M cycloheximide for prolonged periods resulted in inhibition of conjugate formation, but when this concentration of cycloheximide was maintained for only 24 h a significantly (P less than 0.001) increased rate of conjugate formation was observed. The results indicate that metabolic processes in the organ-culture system are in a state of dynamic equilibrium and that morphologic integrity and specific hepatocytic function are maintained after 21 days in vitro. Preferential taurocholate formation was demonstrated in rat fetal liver, and the data suggest that glycine and taurine interact with separate enzymatic systems in bile acid conjugation. The possible mechanisms that mediate the effect of cycloheximide are discussed.