ABSTRACT
AIM/INTRODUCTION: Analgesic, anti-inflammatory and anti-apoptotic effects of pregabalin have been shown previously. In this study, we investigated the protective effect of different doses of pregabalin on skeletal muscle IR injury in rats. MATERIALS AND METHODS: 24 rats were randomly divided into 4 groups (Control, Ischaemia-Reperfusion (IR), IR-Pregabalin 50 mg, IR-Pregabalin 200 mg). Following IR, serum Ischemia Modified Albumin (IMA) and tissue Paraoxonase (PON) were studied and gastrocnemius muscle tissue was removed for histopathologic examination. RESULTS: Interstitial inflammation was higher in the IR group than in the control and Pregabalin 200 mg groups (p = 0.037, p = 0.037, respectively). Congestion was higher in the IR group than in the control, Pregabalin 50 and 200 mg groups (p = 0.001, p = 0.004, p = 0.004, respectively). PON was lower in the IR group than in the Control, Pregabalin 50 and 200 mg groups (p = 0.001, p = 0.007, p = 0.015, respectively). IMA was higher in the IR group than in the Control, Pregabalin 50 and 200 mg groups (p < 0.0001, all). CONCLUSION: We think that administration of pregabalin, more prominent at 200 mg, can reverse the injury that occurs in the skeletal muscle of IR-induced rats. Pregabalin can be safely used for analgesia in cases of IR (Tab. 2, Fig. 9, Ref. 41).
Subject(s)
Pregabalin/pharmacology , Reperfusion Injury/drug therapy , Animals , Apoptosis/drug effects , Biomarkers/blood , Disease Models, Animal , Male , Muscle, Skeletal/pathology , Oxidative Stress/drug effects , Rats , Rats, Wistar , Reperfusion Injury/blood , Serum Albumin , Serum Albumin, HumanABSTRACT
OBJECTIVES: To evaluate the antioxidative and therapeutic effects of spirulina on the trichloroethylene induced cutaneous irritation balb/c mice. BACKGROUND: During recent years, an attention has been focused on the antioxidant potential of Spirulina species. METHODS: Balb/c mice were randomized into the four groups. At the end of the each application, the rats were sacrificed and dorsal skin was taken. Histopathologic and immunohistochemical evaluations were conducted, oxidative stress was assessed by the measurement of malondialdehyde (MDA) levels, superoxide dismutase (SOD) activities and nitric oxide (NO) production. RESULTS: There was a statistically significant decreased disruption in epidermal integrity, oedema in intercellular dermis, disorganization in collagen fibres and immunoreactivity in the pre acute dermatitis/ antioxidant and the post acute dermatitis/ treatment groups when compared to the acute dermatitis group (p<0.05). CONCLUSION: The results of the present study indicate the antioxidative and therapeutic effects of Spirulina on trichloroethylene induced cutaneous irritation balb/c mice (Tab. 2, Fig. 8, Ref. 33).
