Experimental study of the diagnostic potentialities of bioimpedance measurement in acute intestinal obstruction.

The dynamics of impedance values of the small and large intestine, parietal peritoneum, and greater omentum was analyzed during different periods of experimental acute intestinal obstruction. The impedance was below the normal, which indicated progressive microcirculatory disorders and necrosis of the intestinal wall. The histomorphological picture of healing of anastomoses created after resection of the intestine with consideration for bioimpedance values and of anastomoses created after resection of the intestine in a priori viable tissues virtually did not differ.

[Experimental substantiation of use of sodium hypochlorite and ozone at a formation of intestinal anastomosis in conditions of acute intestinal obstruction and peritonitis].

Presented are the results of experimental research on 144 animals (the rats Wistar), on whom was carried out resection of a part of large intestine and a intestinal anastamosis was formed in the presents of acute intestinal obstruction and peritonitis. The sanitation of abdominal cavity in the basic group of animals (74) was performed using sodium hypochlorite solution and dissolved ozone. The intestinal lavage was carried out by dissolved ozone with the subsequent introduction of ozonized oil Ozonide in the area of intestinal anastamosis. The combined application of ozone and sodium produces an expressed samative effect which leads to healing of intestinal anastamosis and decreases unstability of intestinal sutures and mortality.

[Seasonal patterns of breast tumor growth in Far North residents].

Earlier, we established a relationship between sex hormone receptor concentration in tumor and 5-year survival, on the one hand, and seasonality, on the other. The parameters showed a distinct 6-month cycle. That pointed to certain environmental factors which could synchronize hormone-dependent tumor process in the breast of women living in the North. The present study is concerned with a relationship of 6-month rhythm of tumor growth and latitude of residence. Said rhythm was reliably identified as a parameter of 5-year survival in the Far North (68 deg. northern latitude, p < 0.001). Maximum values of 5-year survival were registered in those diagnosed with cancer in winter or summer, while those diagnosed in spring or fall had unfavorable prognosis. Northern magnetic storms recur at 6-month intervals and most frequently in spring and fall. Electromagnetic radiation is known to suppress melatonin production and, that might have stimulated tumor process. Therefore, it is most likely that solar electromagnetic radiation might synchronize hormone-dependent tumor process in women resident in the North.

[The factors affecting the level of ovarian hormone receptors in breast tumors in women]. / Faktory, vliiaiushchie na soderzhanie retseptorov ovarial'nykh gormonov v opukholiakh molochnoi zhelezy u zhenshchin.

We studied the level of estradiol and progesterone receptors in breast tumors of female patients. We have shown that the level of these receptors in tumors depends on whether the tumour is present in the left or right breast, the age of the patient, and the season of the year.

[Application of plasma scalpel for cholecystectomy]. / Kholetsist'ektomiia s primeneniem plazmennogo skal'pelia.

Plasma scalpel SUPR-M was used in operative treatment of calculous cholecystitis. Two variants of cholecystectomy with plasma scalpel have been worked out in experiment. It was determined, that the influence of plasma spurt is innocuous for hepatic tissues, it helps to achieve complete demucosation of the mucous membrane of the gall bladder in atypical variants of cholecystectomy, prevents bleeding and bile leakage from the bladder bead in typical cholecytectomy. In clinics the operation procedures were carried out in 176 patients, in 20 of them cholecystectomy was performed atypically, leaving gall bladder wall, adjacent to hepatic surface, in place. In postoperative period were no complications connected with the use of plasma scalpel as well as there were no lethal outcomes. Long-term follow-up of the patients for 5 years showed favourable clinical effect.

[Proteins associated with nucleolar organizer regions: practical application in tumor histopathology and relationship to biological properties of the tumor]. / Belki, assotsiirovannye s zonami iadryshkovogo organizatora: prakticheskoe primenenie v onkomorfologii i sviaz' s biologicheskimi osobennostiami opukholi.

The argyrophilic staining of nucleolar organizer regions (AgNOR) has been found to be of increasing use in routine and experimental histopathology in the study of certain neoplastic lesions. The AgNOR reaction demonstrates the presence of argyrophilic non-histone proteins within NORs. The number of detectable NORs depends on several factors: the level of transcriptional activity, the number of NOR-bearing chromosomes in the karyotype, and the stage of the cell cycle in which they are sought. In man, five chromosomes have NORs, and these areas are the sites which hybridize with rRNA and are of importance with respect to the ultimate synthesis of protein. The results of AgNORs might be more informative if this technique would be applied simultaneously in cytological and histological specimens for each patient. It may also be important to examine the AgNORs profiles in stromal cells.

[Characteristics of the morphologic changes in an experimental suppurative wound of the perimandibular soft tissues treated by some types of laser radiation]. / Osobennosti morfologicheskikh izmenenii v gnoinoi rane okolocheliustnykh miagkikh tkanei v éksperimente pri lechenii nekotorymi vidami lazernogo izlucheniia.

In rabbits isolated CO2 and He-Ne laser irradiation promotes early granulation of wounds and accelerates epithelialization with more complete restitution of the regenerated skin organ specificity. The best effect was achieved when combined laser irradiation was applied. This was further corroborated by the immunohistochemical investigation.

[Experimental validation of the expediency of the use of ultra-high frequency therapy in combination with antibacterial drugs in infiltrative tuberculosis of the lungs]. / Eksperimental'noe obosnovanie tselesobraznosti primeneniia UVCh-terapii v sochetanii s antibakterial'nymi preparatami pri infil'trativnom tuberkuleze legkikh.

The influence of the U.H.F. electric field in combination with specific antibacterial therapy on the character of morphological shifts, isoniazid accumulation, collagen fractional composition and tubercle bacilli population in the foci of infiltrative tuberculosis of the lungs was studied experimentally. The effect of this preformed physical factor on the process of tuberculosis was shown to be favourable. Under the action of the U.H.F. electric field microcirculation in the lungs improved, and the levels of isoniazid accumulation in tuberculosis foci increased 1.9-fold leading to decreasing of mycobacterial populations. By restricting specific inflammation, the U.H.F. electric field prevented excessive collagen formation.

[Role of modern methods of morphological research in the exact definition of the biological characteristics and morphogenesis of human breast cancer]. / Rol' sovremennykh metodov morfologicheskogo issledovaniia v utochnenii biologicheskikh osobennostei i morfogeneza raka molochnoi zhelezy cheloveka.

Combined use of modern methods of morphologic investigation will allow one in every diagnosed case of breast carcinoma to determine its histogenesis, make a differential diagnosis. Knowledge of biological features of malignant cells will allow one to apply an individual approach to treatment and prognosis. To clarify the morphogenesis of breast carcinoma, it is important to study the stromal component, as parenchymal-stromal relationship is a fundamental link in structural homeostasis.