[Premature aging in sleep apnea comorbid obesity persons.]

The objective was to assess the role of pathological changes in cerebral hemodynamics in obstructive sleep apnea comorbid obesity patients in premature aging. Concentric-eccentric remodeling of the common carotid artery wall, hyperconstrictor variant of cerebral angiopathy, depletion of compensatory mechanisms of cerebral blood flow autoregulation, restriction of hemodynamic reserve with narrowing of the «corridor¼ of admissible changes in cerebral perfusion in patients with respiratory disturbances comorbid obesity. The biological age of sleep apnea patients exceeded the passport age by 41,3%, and comorbid patients by 49,6%. The most significant predictors of premature aging in both groups are cerebral hemodynamic indicators, reflecting the structural and geometric characteristics of the vessels, in particular, the thickness of the intima-media complex of the common carotid artery, as well as microvasculature reactivity indicators. In comorbid individuals, an additional marker of early aging may be the cerebral blood flow index. Patients with sleep apnea and obesity are characterized by accelerated rates of aging. Indicators of cerebral hemodynamics can be considered as markers of premature aging.

[SUPPRESSION OF TUMOR GROWTH AFTER XENOGRAFTING OF HUMAN COLORECTAL CARCINOMA CELLS].

Previously we've described the obtainment of a subpopulation of cancer stem cells from a human colorec- tal carcinoma cell line MIP101. These cells possess elevated clonogenic and tumorigenic capacities. According to our data, depletion of stem compartment in a cancer cell population blocks its tumorigenicity. The current work is dedicated to the comparison of tumorigenic potential between cell populations with enriched or depleted stem compartment. We show that tumor growth following xenografting of enriched stem cell population can be suppressed by intramuscular injections of ganciclovir. Thus, we report a method to obtain a cell population with high Oct4 promoter expression within the MIP101 colorectal carcinoma cell line and to eliminate these cells from the population in vitro as well as in vivo.

[A comparative analysis of colorectal carcinoma cell lines that differ in metastatic potential].

In the current work we make an attempt to compare cancer cells of one origin, but differing in the expression of CEA protein, a clinical marker of metastatic carcinomas, presumably one of the key factors in metastatic activity. We have explored the morphology of cell colonies in vitro, expression patterns of epithelial markers, the ability of these cells to form tumors and metastases in vivo, and evaluated their stem compartment with the aid of a suicidal genetic construct sensitive to the embryonic stem cell marker, Oct4.

[In vitro derivation and characterization of a colorectal cancer stem cell subpopulation].

In present publication we describe for the first time the obtainment of cancer stem cells from a weakly metastatic human colorectal carcinoma cell line MIP101 via selecting from the native population the cells that express intensively an embryonic stem cell marker, POU5F1 (Oct4). We provide the evidence that these cells possess an elevated clonogenic and tumorigenic potential when compared to the native population, and this correlates to the hypothesis of cancer stem cells' primary role in the development of malignant neoplasms.

Vagal reactivity and affective adjustment in infants during interaction challenges.

Respiratory sinus arrhythmia (RSA) and heart period were evaluated in 5-month-old infants (N = 40) during interaction challenges requiring affective adjustment. The paradigm consisted of four 2-min experimental conditions designed to elicit behavioral and autonomic responses to object-mediated (Picture Attention and Toy Attention) and person-mediated (Still Face and Social Interaction) engagement. The data demonstrated that autonomic state systematically changed during engagement and disengagement with the environment. During the object-mediated challenge, increases in RSA were uniquely related to positive engagement. During the person-mediated challenge, there was a more complex integration of autonomic and behavioral responses characterized by concordant increases and decreases in RSA, heart period, positive engagement, negative affect, and motor activity. When participants were partitioned into two groups, based on their RSA response pattern during the person-mediated challenge, only participants who exhibited a pattern of RSA decrease from Toy Attention to Still Face followed by a rapid recovery during Social Interaction demonstrated regulation of behavioral activity, including concordant recovery from stress. These findings provide additional empirical support for the role of vagal regulation of the heart in the modulation of affective adjustment and engagement behavior.

[Micropsychiatry and the possibilities for correcting mental disorders in infancy]. / Mikropsikhiatriia i vozmozhnosti korrektsii psikhicheskikh rasstroistv v mladenchestve.

The investigations of authors concerning infants and their families were generalized on the basis of the observations of infants and their parents with some psychological problems in families. The principles of psychotherapeutic work in above-mentioned groups were formulated. Besides the methods of the diagnosis of psychical deviations development in first year infants as well as peculiarities of alterations in mother-baby system and the main forms of psychic disturbances correction in infants were also described.

[The prevention of mental pathology at an early age]. / O profilaktike piskhicheskoi patologii v rannem vozraste.

New principles of family medical-psychological and psychotherapeutic correction of infants and their parents are presented. Main approaches to prevention of psychic disorders in early childhood are formulated and main tasks for different specialists are specified. Organizational structures such as primary preventive centers based on joint activities of physicians, psychologists and pedagogues are offered.