ABSTRACT
A total of 234 M. tuberculosis isolates were used to demonstrate the leading role of mutations in, respectively, codon 531 of gene rpoB (90.0%) and codon 315 of gene katG (92.9%), in the development of resistance to rifampicin and isoniazid by the methods of reverse hybridization with oligonucleotide probes and the sequencing of gene stretches. The levels of primary resistance of M. tuberculosis to rifampicin, isoniazid and multiresistance, according to the molecular-genetic analysis, were 41.0%, 57.7% and 37.2% respectively. The coincidence of the results of the bacteriological and molecular-genetic analyses of the antimicrobial resistance of the isolates was 90.4% and 95.3% for isoniazid and rifampicin respectively. The prevalence of individual types of mutations, linked with antimicrobial resistance, in the presence of a considerable spread of strains of the family Beijing in the region may be indicative of the limited number of M. tuberculosis clones circulating in the region.
Subject(s)
Drug Resistance, Multiple, Bacterial/genetics , Mycobacterium tuberculosis/genetics , Antibiotics, Antitubercular/pharmacology , Bacterial Proteins/genetics , Catalase/genetics , Codon , DNA-Directed RNA Polymerases/genetics , Humans , Isoniazid/pharmacology , Mutation , Mycobacterium tuberculosis/drug effects , Mycobacterium tuberculosis/isolation & purification , Oligonucleotide Probes , Rifampin/pharmacology , Russia , Tuberculosis/microbiologyABSTRACT
Results of molecular-genetic analysis of Mycobacterium tuberculosis strains isolated from patients of Odessa and Nikolaev regions of Ukraine are represented. Occurrence of individual types of mutations in genes associated with rifampicin and isoniazid resistance has been studied for the first time. Data concerning prevalence of Beijing strains in the South of Ukraine are obtained.
Subject(s)
Bacterial Proteins/genetics , Catalase/genetics , DNA, Bacterial/analysis , Mycobacterium tuberculosis/genetics , Oxidoreductases/genetics , Tuberculosis, Multidrug-Resistant/microbiology , Antitubercular Agents/therapeutic use , Codon/genetics , DNA Fingerprinting , Drug Resistance, Multiple, Bacterial/genetics , Humans , Mutation , Mycobacterium tuberculosis/isolation & purification , Polymerase Chain Reaction , Tuberculosis, Multidrug-Resistant/drug therapy , Tuberculosis, Multidrug-Resistant/epidemiology , Ukraine/epidemiologySubject(s)
Immune System/physiology , Sports/physiology , Adolescent , Adult , Humans , Male , Middle AgedABSTRACT
Activity of peroxidase (AP) and maintenance of lysosomal cation proteins (CP) were studied during phagocytic reaction of polymorphonuclear leukocytes of healthy persons of different age and those with acute respiratory disease and chronic tonsillitis. It is found that during acute virus and chronic bacterial infection similar changes in AP and CP dynamics are observed, which is correlated with a decrease of phagocytic digesting function.
Subject(s)
Bacterial Infections/blood , Blood Proteins/metabolism , Neutrophils/metabolism , Peroxidases/blood , Phagocytosis/immunology , Pneumonia, Viral/blood , Tonsillitis/blood , Adult , Age Factors , Bacterial Infections/immunology , Child, Preschool , Humans , In Vitro Techniques , Infant , Neutrophils/enzymology , Neutrophils/immunology , Peroxidases/deficiency , Pneumonia, Viral/immunology , Tonsillitis/immunologyABSTRACT
Study of the qualitative and quantitative indicators of reprint smears from the surface of the upper respiratory mucosa in healthy infants and in these with acute respiratory viral infection has shown that migrating polymorphonuclear leukocytes take an active part in the functioning of the barrier of the upper respiratory mucosa at the early stages of human ontogenesis. The cytogram and cytochemical characterization of peprint smear cells from the upper respiratory mucosa in infants afflicted with acute respiratory viral infection are sensitive tests in the assessment of defence potentialities of the child's body.
