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1.
Acta Naturae ; 14(2): 50-61, 2022.
Article in English | MEDLINE | ID: mdl-35923564

ABSTRACT

Serine 339 of the active site of Citrobacter freundii methionine γ-lyase (MGL) is a conserved amino acid in most pyridoxal 5'-phosphate-dependent enzymes of the cystathionine ß-lyase subclass, to which MGL belongs. The reaction mechanism of the MGL-catalyzed γ-elimination reaction is poorly explored. We replaced serine 339 with alanine using site-directed mutagenesis. The replacement of serine 339 with alanine led to a significant (by two orders of magnitude) decrease in efficiency in the catalysis of the γ- and ß-elimination reactions by the mutant form of the enzyme. The exchange rates of the C-α- and C-ß-protons in the amino acids in complexes consisting of the enzyme and competitive inhibitors decreased by one-two orders of magnitude. The spectral characteristics of the mutant form indicated that the replacement did not lead to significant changes in the conformation and tautomerism of MGL internal aldimine. We crystallized the holoenzyme and determined its spatial structure at 1.7 E resolution. The replacement of serine 339 with alanine did not affect the overall course of the polypeptide chain of the MGL subunit and the tetrameric enzyme structure. An analysis of the obtained kinetic and spectral data, as well as the known spatial structures of C. freundii MGL, indicates that serine 339 is necessary for efficient catalysis of γ- and ß-elimination reactions at the stage of C-α-proton abstraction from the external aldimine, the γ-elimination reaction at the stages of coenzyme C4'-atom protonation, and C-ß-proton abstraction from a ketimine intermediate.

2.
J Biomol Struct Dyn ; 35(4): 704-723, 2017 Mar.
Article in English | MEDLINE | ID: mdl-26987269

ABSTRACT

In the present paper, the interactions of the origin binding protein (OBP) of herpes simplex virus type 1 (HSV1) with synthetic four-way Holliday junctions (HJs) were studied using electrophoresis mobility shift assay and the FRET method and compared with the interactions of the protein with duplex and single-stranded DNAs. It has been found that OBP exhibits a strong preference for binding to four-way and three-way DNA junctions and possesses much lower affinities to duplex and single-stranded DNAs. The protein forms three types of complexes with HJs. It forms complexes I and II which are reminiscent of the tetramer and octamer complexes with four-way junction of HJ-specific protein RuvA of Escherichia coli. The binding approaches saturation level when two OBP dimers are bound per junction. In the presence of Mg2+ ions (≥2 mM) OBP also interacts with HJ in the stacked arm form (complex III). In the presence of 5 mM ATP and 10 mM Mg2+ ions OBP catalyzes processing of the HJ in which one of the annealed oligonucleotides has a 3'-terminal tail containing 20 unpaired thymine residues. The observed preference of OBP for binding to the four-way DNA junctions provides a basis for suggestion that OBP induces large DNA structural changes upon binding to Box I and Box II sites in OriS. These changes involve the bending and partial melting of the DNA at A+T-rich spacer and also include the formation of HJ containing Box I and Box II inverted repeats and flanking DNA sequences.


Subject(s)
DNA Replication , DNA, Cruciform/antagonists & inhibitors , DNA, Viral/genetics , DNA-Binding Proteins/pharmacology , Herpesvirus 1, Human , Base Sequence , DNA, Viral/metabolism , Electrophoretic Mobility Shift Assay , Humans , Nucleic Acid Conformation
3.
Biofizika ; 61(2): 270-6, 2016.
Article in Russian | MEDLINE | ID: mdl-27192828

ABSTRACT

The binding of distamycin dimeric analog (Pt-bis-Dst) to poly[d(A-T)] x poly[d(A-T)1, poly(dA) x poly(dT) and duplex O23 with the sequence 5'-GCCAATATATATATATTATTAGG-3' which is present at the origin of replication of herpes simplex virus OriS is investigated with the use of UV and CD spectroscopy. The distinction of the synthetic polyamide from a natural antibiotic lies in the fact that in the synthetic polyamide there are two distamycin moieties bound via a glycine cis-diamino platinum group. It was shown that the binding of Pt-bis-Dst to poly[d(A-T)] x poly[d(A-T)] and poly(dA) x poly(dT) reaches saturation if one molecule of the ligand occurs at approximately every 8 bp. With further increase in the ratio of the added ligand to the base pairs in CD spectra of complexes with poly[d(A-T)] x poly[d(A-T)], we observed that the maximum wavelength band tend to be shifted towards longer wavelengths, while in the spectral region of 290-310 nm a "shoulder", that was absent in the spectra of the complexes obtained at low polymer coverages by the ligand, appeared. At high molar concentration ratios of ligand to oligonucleotide Pt-bis-Dst can bind to poly[d(A-T)] x poly[d(A-T)] in the form of hairpins or may form associates by the interaction between the distamycin moieties of neighboring molecules of Pt-bis-Dst. The structure of the complexes is stabilized by interactions between pirrolcarboxamide moieties of two molecules of Pt-bis-Dst adsorbed on adjacent overlapping binding sites. These interactions are probably also responsible for the concentration-dependent spectral changes observed during the formation of a complex between Pt-bis-Dst and poly[d(A-T)] x poly[d(A-T)]. Spectral changes are almost absent in binding of Pt-bis-Dst to poly(dA) x poly(dT). Binding of Pt-bis-Dst to duplex O23 reaches saturation if two ligand molecules occur in a duplex that contains a cluster of 18 AT pairs. With increasing the molar concentration ratio of the ligand to the duplex CD spectra undergo concentration-dependent changes similar to those observed during binding of Pt-bis-Dst to poly [d(A-T)] x poly[d(A-T)]. Testing for antiviral efficacy of Pt-bis-Dst showed that the concentration, at which the cytopathic effect produced by the herpes simplex virus in cell culture Vero E6 halved, is equal to 1.5 µg/ml and the selectivity index for evaluating antiviral activity is 65 at a relatively low cytotoxicity. The concentration of Pt-bis-Dst, at which approximately half the cells are killed, is equal to 100 µg/ml.


Subject(s)
DNA Replication/genetics , Replication Origin/genetics , Simplexvirus/chemistry , Circular Dichroism , Nucleic Acid Conformation , Oligonucleotides/chemistry , Poly A/chemistry , Poly A/genetics , Poly T/chemistry , Poly T/genetics
4.
Acta Naturae ; 7(4): 128-35, 2015.
Article in English | MEDLINE | ID: mdl-26798500

ABSTRACT

The problem of resistance to antibiotics requires the development of new classes of broad-spectrum antimicrobial drugs. The concept of pro-drugs allows researchers to look for new approaches to obtain effective drugs with improved pharmacokinetic and pharmacodynamic properties. Thiosulfinates, formed enzymatically from amino acid sulfoxides upon crushing cells of genus Allium plants, are known as antimicrobial compounds. The instability and high reactivity of thiosulfinates complicate their use as individual antimicrobial compounds. We propose a pharmacologically complementary pair: an amino acid sulfoxide pro-drug and vitamin B6 - dependent methionine γ-lyase, which metabolizes it in the patient's body. The enzyme catalyzes the γ- and ß-elimination reactions of sulfoxides, analogues of L-methionine and L-cysteine, which leads to the formation of thiosulfinates. In the present work, we cloned the enzyme gene from Clostridium sporogenes. Ionic and tautomeric forms of the internal aldimine were determined by lognormal deconvolution of the holoenzyme spectrum and the catalytic parameters of the recombinant enzyme in the γ- and ß-elimination reactions of amino acids, and some sulfoxides of amino acids were obtained. For the first time, the possibility of usage of the enzyme for effective conversion of sulfoxides was established and the antimicrobial activity of thiosulfinates against Gram-negative and Gram-positive bacteria in situ was shown.

5.
J Biomol Struct Dyn ; 32(9): 1456-73, 2014.
Article in English | MEDLINE | ID: mdl-23879454

ABSTRACT

The herpes simplex virus type 1 origin-binding protein, OBP, is a DNA helicase encoded by the UL9 gene. The protein binds in a sequence-specific manner to the viral origins of replication, two OriS sites and one OriL site. In order to search for efficient inhibitors of the OBP activity, we have obtained a recombinant origin-binding protein expressed in Escherichia coli cells. The UL9 gene has been amplified by PCR and inserted into a modified plasmid pET14 between NdeI and KpnI sites. The recombinant protein binds to Box I and Box II sequences and possesses helicase and ATPase activities. In the presence of ATP and viral protein ICP8 (single-strand DNA-binding protein), the initiator protein induces unwinding of the minimal OriS duplex (≈80 bp). The protein also binds to a single-stranded DNA (OriS*) containing a stable Box I-Box III hairpin and an unstable AT-rich hairpin at the 3'-end. In the present work, new minor groove binding ligands have been synthesized which are capable to inhibit the development of virus-induced cytopathic effect in cultured Vero cells. Studies on binding of these compounds to DNA and synthetic oligonucleotides have been performed by fluorescence methods, gel mobility shift analysis and footprinting assays. Footprinting studies have revealed that Pt-bis-netropsin and related molecules exhibit preferences for binding to the AT-spacer in OriS. The drugs stabilize structure of the AT-rich region and inhibit the fluctuation opening of AT-base pairs which is a prerequisite to unwinding of DNA by OBP. Kinetics of ATP-dependent unwinding of OriS in the presence and absence of netropsin derivatives have been studied by measuring the efficiency of Forster resonance energy transfer (FRET) between fluorophores attached to 5'- and 3'- ends of an oligonucleotide in the minimal OriS duplex. The results are consistent with the suggestion that OBP is the DNA Holiday junction (HJ) binding helicase. The protein induces conformation changes (bending and partial melting) of OriS duplexes and stimulates HJ formation in the absence of ATP. The antiviral activity of bis-netropsins is coupled with their ability to inhibit the fluctuation opening of АТ base pairs in the А + Т cluster and their capacity to stabilize the structure of the АТ-rich hairpin in the single-stranded oligonucleotide corresponding to the upper chain in the minimal duplex OriS. The antiviral activities of bis-netropsins in cell culture and their therapeutic effects on HSV1-infected laboratory animals have been studied.


Subject(s)
Antiviral Agents/chemistry , DNA Helicases/chemistry , DNA Replication , DNA, Viral/chemistry , DNA-Binding Proteins/chemistry , Herpesvirus 1, Human/metabolism , Netropsin/chemistry , Viral Proteins/chemistry , Animals , Antiviral Agents/pharmacology , DNA Helicases/genetics , DNA, Viral/metabolism , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Netropsin/analogs & derivatives , Netropsin/pharmacology , Organoplatinum Compounds/chemistry , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Structure-Activity Relationship , Vero Cells , Viral Proteins/genetics , Viral Proteins/metabolism
6.
Biofizika ; 57(2): 232-42, 2012.
Article in Russian | MEDLINE | ID: mdl-22594278

ABSTRACT

Data obtained show that antiviral activities of bis-linked netropsin derivatives are targeted by specific complexes formed by helicase UL9 of herpes simplex virus type 1 with viral DNA replication origins, represented by two OriS sites and one OriL site. According to the results of footprinting studies bis-netropsins get bound selectively to an A+T-cluster which separates interaction sites I and II for helicase UL9 in OriS. Upon binding to DNA bis-netropsins stabilize a structure of the A+T-cluster and inhibit thermal fluctuation-induced opening of AT- base pairs which is needed for local unwinding of DNA by helicase UL9. Kinetics of ATP-dependent DNA unwinding in the presence and absence of Pt-bis-netropsin are studied by measuring the efficiency of Forster resonance energy transfer (FRET) between the fluorescent probes attached covalently to 3?- and 5?-ends of the oligonucleotides in the minimal OriS duplex. Pt-bis-netropsin and related molecules inhibit unwinding of OriS duplex by helicase UL9. Pt-bis-netropsin is also able to reduce the rate of unwinding of the AT- rich hairpin formed by the upper strand in the minimal OriS duplex. The antiviral activities and toxicity of bis-linked netropsin derivatives are studied in cell cultured experiments and experiments with animals infected by herpes virus.


Subject(s)
Antiviral Agents/pharmacology , DNA Replication/drug effects , DNA, Viral/metabolism , DNA-Binding Proteins , Herpes Simplex , Herpesvirus 1, Human/enzymology , Netropsin/pharmacology , Viral Proteins , Animals , Chlorocebus aethiops , DNA-Binding Proteins/antagonists & inhibitors , DNA-Binding Proteins/metabolism , Herpes Simplex/drug therapy , Herpes Simplex/enzymology , Mice , Mice, Inbred BALB C , Netropsin/analogs & derivatives , Vero Cells , Viral Proteins/antagonists & inhibitors , Viral Proteins/metabolism
7.
Biochemistry (Mosc) ; 75(10): 1272-80, 2010 Oct.
Article in English | MEDLINE | ID: mdl-21166645

ABSTRACT

Kinetic parameters of Citrobacter freundii methionine γ-lyase were determined with substrates in γ-elimination reactions as well as the inhibition of the enzyme in the γ-elimination of L-methionine by amino acids with different structure. The data indicate an important contribution of the sulfur atom and methylene groups to the efficiency of binding of substrates and inhibitors. The rate constants of the enzyme-catalyzed exchange of C-α- and C-ß-protons with deuterium were determined, as well as the kinetic isotope effect of the deuterium label in the C-α-position of inhibitors on the rate of exchange of their ß-protons. Neither stereoselectivity in the ß-proton exchange nor noticeable α-isotope effect on the exchange rates of ß-protons was found. The ionic and tautomeric composition of the external Schiff base of methionine γ-lyase was determined. Spectral characteristics (absorption and circular dichroism spectra) of complexes with substrates and inhibitors were determined. The spectral and kinetic data indicate that deamination of aminocrotonate should be the rate-determining stage of the enzymatic reaction.


Subject(s)
Amino Acids/chemistry , Bacterial Proteins/chemistry , Carbon-Sulfur Lyases/chemistry , Citrobacter freundii/enzymology , Amino Acids/metabolism , Bacterial Proteins/metabolism , Carbon-Sulfur Lyases/metabolism , Kinetics , Substrate Specificity/physiology
8.
Biofizika ; 55(2): 239-51, 2010.
Article in Russian | MEDLINE | ID: mdl-20429277

ABSTRACT

The protein binding to the origin of replication of the herpes simplex virus type 1 (HSV-1) is DNA helicase encoded by the UL9 gene of the herpes virus. The protein specifically binds to two binding sites in the viral DNA replication origins OriS or OriL. In order to determine the role of the UL9 protein in the initiation of replication and find efficient inhibitors of the UL9 activity, we have synthesized a recombinant UL9 protein expressed in E. coli cells. It was found that the recombinant UL9 protein binds to Boxes I and II in the OriS and possesses the DNA helicase and ATPase activities. In a complex with a fluorescent analog of ATP, two molecules of the ATP analog bind to one protein dimer molecule. It was also found that the UL9 protein in the dimer form can bind simultaneously to two DNA fragments, each containing specific binding sites for the protein. The interaction of the recombinant UL9 protein with the 63-mer double and single-stranded oligonucleotides OriS and OriS* has been investigated, which correspond to the origin of replication of herpes simplex virus. From the titrations of OriS and OriS* by ethidium bromide in the presence and absence of the UL9 protein, the equilibrium affinity constants of the protein binding to OriS and OriS* have been determined. A DNase I footprinting study showed that bis-linked netropsin derivatives exhibit preferences for binding to the AT-cluster in the origin of replication OriS and inhibit the fluctuation opening of AT-base pairs in the AT-cluster. The drugs also prevent the formation of an intermediate conformation of OriS* that involves a disordered tail at the 3'-end and stable Box I-Box III hairpin to which the UL9 helicase selectively binds. The stabilization by bis-netropsins of the AT-rich hairpin at its 3' end can inhibit the helicase activity. It was concluded that the antiviral activity of bis-netropsins may be associated with the inhibitory effects of bis-netropsins on these two stages of the reaction catalyzed by helicase UL9.


Subject(s)
Antiviral Agents/chemistry , DNA Helicases/chemistry , DNA, Viral/chemistry , DNA-Binding Proteins/chemistry , Herpesvirus 1, Human/enzymology , Netropsin/analogs & derivatives , Viral Proteins/chemistry , Adenosine Triphosphate/chemistry , Adenosine Triphosphate/metabolism , Antiviral Agents/therapeutic use , DNA Helicases/genetics , DNA Helicases/metabolism , DNA-Binding Proteins/genetics , Herpes Simplex/drug therapy , Herpes Simplex/enzymology , Herpesvirus 1, Human/genetics , Netropsin/chemistry , Netropsin/therapeutic use , Protein Binding/drug effects , Protein Binding/physiology , Protein Multimerization/drug effects , Protein Multimerization/genetics , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Replication Origin/physiology , Viral Proteins/genetics
9.
J Biomol Struct Dyn ; 26(6): 701-18, 2009 Jun.
Article in English | MEDLINE | ID: mdl-19385699

ABSTRACT

In the present work, we employed UV-VIS spectroscopy, fluorescence methods, and circular dichroism spectroscopy (CD) to study the interaction of dye Hoechst 33258, Hoechst 33342, and their derivatives to poly[d(AT)].poly[d(AT)], poly(dA).poly(dT), and DNA dodecamer with the sequence 5'-CGTATATATACG-3'. We identified three types of complexes formed by Hoechst 33258, Hoechst 33342, and methylproamine with DNA, corresponding to the binding of each drug in monomer, dimer, and tetramer forms. In a dimer complex, two dye molecules are sandwiched in the same place of the minor DNA groove. Our data show that Hoechst 33258, Hoechst 33342, and methylproamine also form complexes of the third type that reflects binding of dye associates (probably tetramers) to DNA. Substitution of a hydrogen atom in the ortho position of the phenyl ring by a methyl group has a little effect on binding of monomers to DNA. However it reduces strength of binding of tetramers to DNA. In contrast, a Hoechst derivative containing the ortho-isopropyl group in the phenyl ring exhibits a low affinity to poly(dA).poly(dT) and poly[d(AT)].poly[d(AT)] and binds to DNA only in the monomer form. This can be attributed to a sterical hindrance caused by the ortho-isopropyl group for side-by-side accommodation of two dye molecules in the minor groove. Our experiments show that mode of binding of Hoechst 33258 derivatives and their affinity for DNA depend on substituents in the ortho position of the phenyl ring of the dye molecule. A statistical mechanical treatment of binding of Hoechst 33258 and its derivatives to a polynucleotide lattice is described and used for determination of binding parameters of Hoechst 33258 and its derivatives to poly[d(AT)].poly[d(AT)] and poly(dA).poly(dT).


Subject(s)
Benzimidazoles/chemistry , Bisbenzimidazole/chemistry , DNA/chemistry , Base Sequence , Binding Sites , Binding, Competitive , Circular Dichroism , Kinetics , Models, Chemical , Models, Molecular , Molecular Structure , Nucleic Acid Conformation , Oligonucleotides/chemistry , Poly T/chemistry , Poly dA-dT/chemistry , Spectrometry, Fluorescence , Spectrophotometry, Ultraviolet , Thermodynamics
10.
Biofizika ; 53(5): 744-53, 2008.
Article in Russian | MEDLINE | ID: mdl-18954000

ABSTRACT

The binding to DNA of Pt-bis-Nt and its modified analogue (Pt*-bis-Nt), which differs from Pt-bis-Nt by the fact that the connecting chain between two netropsin fragments contains two additional glycine residues, has been studied. Elongating the chain in the bis-netropsin molecule increases the cytotoxicity and leads to a complete disappearance of the antiherpetic activity of bis-netropsin. A study of the binding of two bis-netropsins with the oligonucleotide duplex containing an AT cluster, which is present at the replication initiation site of herpes virus (OriS), revealed significant structural differences between complexes of bis-netropsins with this DNA oligomer. It was shown by CD spectroscopy that the binding of Pt-bis-Nt in the elongated conformation and in the form of a hair-pin with the parallel orientation of two bis-netropsin fragments makes a greater contribution than it is the case in the complex formation with Pt*-bis-Nt. At high binding rates, Pt*-bis-Nt binds to the AT cluster in OriS predominantly in the form of associates based on the antiparallel double-stranded pyrrolcarboxyamide motif. The interaction of Pt-bis-Nt and Pt*-bis-Nt with the single-stranded oligonucleotide (64 nt), which corresponds to the upper strand at the replication initiation site of herpes virus (OriS*), was also studied. Substantial differences in the binding of bis-netropsins with OriS* and thermostability of the resulting complexes were found by CD spectroscopy and by studying the melting of complexes of bis-netropsins with OriS*.


Subject(s)
Antiviral Agents/chemistry , DNA, Viral/chemistry , Herpesviridae/chemistry , Netropsin/analogs & derivatives , Organoplatinum Compounds/chemistry , Replication Origin , Netropsin/chemistry , Nucleic Acid Conformation , Oligodeoxyribonucleotides/chemistry
11.
Biochim Biophys Acta ; 1764(7): 1268-76, 2006 Jul.
Article in English | MEDLINE | ID: mdl-16793353

ABSTRACT

In the X-ray structure of tyrosine phenol-lyase (TPL) Asp214 is located at H-bonding distance from the N1 atom of the cofactor. This residue has been replaced with Ala and Asn and the properties of the mutant enzymes have been studied. The substitutions result in a decrease in the cofactor affinity of about four orders of magnitude. D214A and D214N TPLs do not catalyze the decomposition of l-Tyr and 3-fluoro-l-Tyr. They decompose substrates, containing better leaving groups with rates reduced by one or two orders of magnitude. Lognormal resolution of the spectra of the mutant enzymes revealed that the N1 atom of the cofactor is deprotonated. Spectral characteristics of internal and external aldimines of the mutant TPLs and the data on their interaction with quasisubstrates demonstrate that replacements of Asp214 lead to alteration of active site conformations. The mutant enzymes do not form noticeable amounts of a quinonoid upon interaction with inhibitors, but catalyze isotope exchange of C-alpha-proton of a number of amino acids for deuterium in (2)H(2)O. The k(ex) values for the isotope exchange of l-phenylalanine and 3-fluoro-l-tyrosine are close to the k(cat) values for reacting substrates. Thus, for the mutant TPLs the stage of C-alpha-proton abstraction may be considered as a rate-limiting for the whole reaction.


Subject(s)
Aspartic Acid/chemistry , Citrobacter freundii/enzymology , Coenzymes/chemistry , Tyrosine Phenol-Lyase/chemistry , Alanine/genetics , Apoenzymes/chemistry , Asparagine/genetics , Aspartic Acid/genetics , Binding Sites/genetics , Catalysis , Circular Dichroism , Citrobacter freundii/genetics , Deuterium Oxide/chemistry , Homoserine/chemistry , Homoserine/genetics , Kinetics , Molecular Structure , Mutation/genetics , Phenylalanine/chemistry , Phenylalanine/genetics , Recombinant Fusion Proteins/chemistry , Spectrophotometry , Tyrosine/chemistry , Tyrosine/genetics , Tyrosine Phenol-Lyase/genetics , Tyrosine Phenol-Lyase/metabolism
12.
Biochemistry (Mosc) ; 68(11): 1181-8, 2003 Nov.
Article in English | MEDLINE | ID: mdl-14640959

ABSTRACT

In the spatial structure of tryptophanase from Proteus vulgaris the guanidinium group of arginine 226 forms a salt bridge with the 3;-oxygen atom of the coenzyme. The replacement of arginine 226 with alanine using site-directed mutagenesis reduced the affinity of the coenzyme for the protein by one order of magnitude compared to the wild-type enzyme. The catalytic activity of the mutant enzyme in the reaction with L-tryptophan was reduced 10(5)-fold compared to the wild-type enzyme. The rates of the reactions with some other substrates decreased 10(3)-10(4)-fold. The mutant enzyme catalyzed exchange of the C-alpha-proton in complexes with some inhibitors with rates reduced 10(2)-fold compared to the wild-type enzyme. Absorption and circular dichroism spectra of the mutant enzyme and the enzyme-inhibitor complexes demonstrate that the replacement of arginine 226 with alanine does not significantly affect the tautomeric equilibrium of the internal aldimine, but it leads to an alteration of the optimal conformation of the coenzyme-substrate intermediates.


Subject(s)
Proteus vulgaris/enzymology , Tryptophanase/chemistry , Tryptophanase/metabolism , Amino Acid Sequence , Amino Acid Substitution , Amino Acids/metabolism , Arginine/chemistry , Catalytic Domain/genetics , Kinetics , Molecular Sequence Data , Molecular Structure , Mutagenesis, Site-Directed , Proteus vulgaris/genetics , Sequence Alignment , Substrate Specificity , Tryptophanase/genetics
14.
Eur J Biochem ; 267(23): 6897-902, 2000 Dec.
Article in English | MEDLINE | ID: mdl-11082202

ABSTRACT

The phosphinic analogues of tyrosine and pyruvate were first demonstrated to be substrates in the reactions of elimination and synthesis catalyzed by tyrosine phenol-lyase. Kinetic parameters of the enzymatic process were determined, and the first enzymic synthesis of an aminophosphinic acid was carried out. Replacement of the planar HOOC-group by the tetrahedral (HO)(O)PH-group in the substrate slightly affected its affinity for the enzyme but substantially diminished the conversion rate. For phosphonic analogues, containing (HO)2(O)P group, the affinity to the enzyme was decreased considerably while the conversion was completely prevented. Thus, the structural parameters of the acid group are important not only for the affinity for the enzyme, but also for the formation of the catalytically competent conformation of the active site.


Subject(s)
Amino Acids/metabolism , Tyrosine Phenol-Lyase/metabolism , Binding Sites , Catalysis , Citrobacter/enzymology , Kinetics , Models, Chemical , Organophosphonates/chemical synthesis , Protein Binding , Pyruvic Acid/metabolism , Substrate Specificity , Tyrosine/metabolism
15.
Eur J Biochem ; 267(6): 1830-6, 2000 Mar.
Article in English | MEDLINE | ID: mdl-10712616

ABSTRACT

Spectral properties of the internal Schiff base in tyrosine phenol-lyase have been investigated in the presence of an activating cation K+ and a cation-inhibitor Na+. The holoenzyme absorption spectra in the pH range 6.5-8.7 were recorded in the presence of K+. No apparent pKa value of the coenzyme chromophore was found in this pH range, indicating that the internal Schiff base does not change its ionic form on going from pH 6.5 to 8.7. To determine the ionic state and tautomeric composition of the Schiff base in tyrosine phenol-lyase, the absorption and circular dichroism spectra were analyzed using lognormal distribution curves. The predominant form of the internal Schiff base is that with protonated pyridinium and aldimine nitrogen atoms and deprotonated 3'-hydroxy group, i.e. the ketoenamine. This form is in prototropic equilibrium with its enolimine tautomer. The internal aldimine ionic form is changed upon replacement of K+ with Na+. This replacement leads to a significant decrease in the pKa value of pyridinium nitrogen of the pyridoxal-P.


Subject(s)
Bacterial Proteins/chemistry , Citrobacter freundii/enzymology , Pyridoxal Phosphate/analysis , Tyrosine Phenol-Lyase/chemistry , Circular Dichroism , Hydrogen-Ion Concentration , Kinetics , Molecular Conformation , Potassium/chemistry , Protein Conformation , Schiff Bases/analysis , Sodium/chemistry , Spectrophotometry
16.
Mol Biol (Mosk) ; 29(1): 135-43, 1995.
Article in Russian | MEDLINE | ID: mdl-7723753

ABSTRACT

An algorithm (with appropriate application package) which enables automatic determination of "reference" CD spectra of different protein secondary structure elements from set of CD spectra of proteins with known contents of the elements with taking into account of "aromatic contribution" is elaborated. By means of these spectra one can determine contents of secondary structure elements in arbitrary proteins and polypeptides.


Subject(s)
Protein Structure, Secondary , Algorithms , Circular Dichroism , Reference Values
17.
FEBS Lett ; 357(1): 58-61, 1995 Jan 02.
Article in English | MEDLINE | ID: mdl-8001679

ABSTRACT

The effect of ethanol and pH on thermodynamic parameters and cooperativity of pepsinogen heat denaturation was studied by scanning microcalorimetry. Addition of 20% ethanol decreases the protein denaturation temperature by 10.7 degrees C at pH 6.4 and 15.8 degrees C at pH 8.0. It also decreases the denaturation heat capacity increment from 5.8 to 4.2 kcal/K.mol. The dependences of calorimetric denaturation enthalpy on denaturation temperature both in water and 20% ethanol are linear and intersect at about 95 degrees C. In 20% ethanol the pH shift from 5.9 to 8.0 results in a decreased number of cooperative domains in pepsinogen. This process causes no changes either in the secondary structure or in the local surroundings of aromatic amino acids. It is concluded that ethanol addition does not affect the cooperativity of pepsinogen denaturation substantially until the pH change provokes redistribution of charges in the protein molecule.


Subject(s)
Pepsinogens/chemistry , Calorimetry, Differential Scanning , Ethanol/pharmacology , Hot Temperature , Hydrogen-Ion Concentration , Pepsinogens/drug effects , Protein Denaturation , Water
18.
Mol Biol (Mosk) ; 28(6): 1346-53, 1994.
Article in Russian | MEDLINE | ID: mdl-7885334

ABSTRACT

The effect of ethanol and medium pH on thermodynamic parameters and cooperativity of pepsinogen thermal denaturation have been studied by scanning microcalorimetry. Addition of 20% (v/v) ethanol decreases the protein temperature of denaturation by 10.7 degrees C at pH 6.4 and by 15.8 degrees C at pH 8.0. It decreases the denaturation heat capacity change of pepsinogen from 5.8 to 4.2 kcal/K.mol, but has no effect on the number of energetic domains (regions melting in an "all-or-none" manner). The dependences of calorimetric denaturation enthalpy on denaturation temperature in both aqueous solution and 20% ethanol are linear and converge at about 95 degrees C, which coincides with the converge temperature of similar dependencies shown for a number of proteins in aqueous and water-alcohol solutions. A change of pH from 5.9 to 8.2 in 20% ethanol has been shown to cause a decrease of the number of cooperatively melting regions in pepsinogen. This process involves no changes either in the secondary structure or in the local surroundings of aromatic amino acids. It is concluded that ethanol addition has no effect on pepsinogen denaturation cooperativity until there is sufficient influence on intramolecular charge distribution, taking place upon a change of pH.


Subject(s)
Ethanol/pharmacology , Pepsinogens/chemistry , Animals , Calorimetry, Differential Scanning , Circular Dichroism , Enzyme Activation , Hot Temperature , Hydrogen-Ion Concentration , Pepsin A/metabolism , Pepsinogens/metabolism , Protein Denaturation , Protein Structure, Secondary , Swine , Thermodynamics
19.
Biofizika ; 39(4): 746-8, 1994.
Article in Russian | MEDLINE | ID: mdl-7981285

ABSTRACT

There is established that the protein trypsin inhibitor Kunitz is a globular protein of (PPII-PPII)-type. The content of poly-L-proline helix is not smaller than 43%. It has been shown by CD method that the transition from an extended left-handed helical structure to alpha-helix arises on heating till the denaturation temperatures. On this assumption the CD spectrum of poly-L-proline II type has been calculated.


Subject(s)
Peptides/analysis , Proteins/chemistry , Trypsin Inhibitor, Kunitz Soybean/chemistry , Circular Dichroism , Computer Graphics , Protein Conformation
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