ABSTRACT
The Kosakonia cowanii Cp1 strain was isolated from seeds of Capsicum pubescens R. & P. cultivated in Michoacan, Mexico. Genetic and ecological role analyses were conducted for better characterization. The results show that genome has a length of 4.7 Mbp with 56.22% G + C and an IncF plasmid of 128 Kbp with 52.51% G + C. Furthermore, pathogenicity test revealed nonpathogenic traits confirmed by the absence of specific virulence-related genes. Interestingly, when fungal inhibitory essays were carried out, the bacterial synthesis of volatile organic compounds (VOCs) with antifungal activity showed that Sclerotinia sp. and Rhizoctonia solani were inhibited by 87.45% and 77.24%, respectively. Meanwhile, Sclerotium rolfsii, Alternaria alternata, and Colletotrichum gloeosporioides demonstrated a mean radial growth inhibition of 52.79%, 40.82%, and 55.40%, respectively. The lowest inhibition was by Fusarium oxysporum, with 10.64%. The VOCs' characterization by headspace solid-phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME-GC-MS) revealed 65 potential compounds. Some of the compounds identified with high relative abundance were ketones (22.47%), represented by 2-butanone, 3-hydroxy (13.52%), and alcohols (23.5%), represented by ethanol (5.56%) and 1-butanol-3-methyl (4.83%). Our findings revealed, for the first time, that K. cowanii Cp1 associated with C. pubescens seeds possesses potential traits indicating that it could serve as an effective biocontrol.
ABSTRACT
Tanto las entidades públicas en salud como las asociaciones médicas se preocupan por mejorar la calidad deprestación de sus servicios olvidando que uno de los factores condicionantes básicos de la productividad es elgrado de complacencia que los profesionales tienen con su trabajo.Fue un estudio observacional descriptivo, de corte transversal, realizado a través del Cuestionario de calidadde vida profesional (CVP-35), validado en español, que consta de treinta y cinco ítems.Se llevó a cabo un total de veinticinco encuestas, edad media: 29 años. Los resultados en la encuesta de calidadde vida revelan en el ítem Trabajo que tengo que el 100% está en valores > 5 (Bastante a Mucho); en Satisfaccióncon el tipo de trabajo el 72% está distribuido en valores > 5 (Bastante a Mucho); en Satisfaccióncon el sueño, 72% fueron calificados con valores < 5 (Algo o Nada); en Calidad de vida percibida 4% lacalificó como Nada, 40% Algo, 44% Bastante y 12% Mucho; y en la variable Estrés emocional,76% puntuó valores > 5 (Bastante a Mucho).El estudio de calidad de vida profesional medida por el cuestionario CVP-35 mostró que la mayoría de residentesviven en estrato socioeconómico 3 y 68% no tienen vivienda propia. Una gran cantidad de residentes trabajanmás de cuarenta y ocho horas semanales. Llama la atención que el 40% reportó sufrir de alguna patología. Lashoras de sueño diarias son siete en promedio, lo cual es aceptable, sin embargo 72% no están satisfechos conlas horas de sueño; pese a que 76% refieren tener una gran cantidad de trabajo y presentar estrés emocional,72% están satisfechos con el tipo de trabajo y más de la mitad de los residentes consideran tener una buenacalidad de vida.
Both public health institutions and medical associations concerned about improving prevision of heathcare, forgetting that one of the basic conditioning factors of productivity is the degree of complacency that professionals have with their workIt was a descriptive study, cross-sectional, conducted through the questionnaire of quality of professional life (CVP-35), validated in spanish, consisting of thirty- five items.A total of twenty survey was conducted, mean age: 29 years. The results in quality of life survey reveal in item "Work I have" that 100% values is > 5 (Quite to Much); In "Satisfaction with the type of work" 72% is distributed in values > 5 (Quite to Much); In "Satisfaction with sleep" 72 % were rated with values < 5 (Something or Nothing); In "Perceived quality of life" 4% qualify as "Nothing", 40% "Something", 44% "Quite" and 12% "Much"; and in the variable "Emotional stress" 76 % scored values > 5 (Quite to Much).The study of professional quality of life measured by the CVP -35 questionnaire showed that most residents live in socioeconomic stratum 3 and 68% not have their own housing. A lot of residents work more than forty- eight hours per week. It is striking that 40 % reported suffering from some pathology. The average of daily hours of sleep is seven, which it is acceptable, however 72 % are dissatisfied with the sleep hours. Although 76 % reported having a lot of work and present emotional stress, 72 % are satisfied with the type of work and more than half of residents believe they have a good quality of life.