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2.
Rev. biol. trop ; 42(1/2): 319-22, abr.-ago. 1994. graf, tab
Article in Spanish | LILACS | ID: lil-218385

ABSTRACT

The effect of irradiation of the aqueous solution of L. m. muta venom was evaluated with thermic neutrons from Uranium-235 employing doses of 1.61 to 5.19 Gray. The venom was examined for protein content by the Folin Lowry Method modified by Stauffer; for acute toxicity by intraperitoneal route estimation in mice and for immunochemical tests by the antigen-antibody reactions evaluation. Neutronic radiation affects all evaluated parameters in venom (decrease in protein content levels, increase of LD50 values and decrease in the number of precipitating antigen-antibody) as shown by immunodiffusion and immunoelectrophoresis


Subject(s)
Animals , Mice , Snake Venoms/radiation effects , Uranium , Viperidae , Radiation Dosage , Immunodiffusion , Immunoelectrophoresis , Lethal Dose 50 , Proteins/radiation effects , Proteins/chemistry , Snake Venoms/chemistry
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