ABSTRACT
It is well known that caloric restriction inhibits, whereas excess calories promote, mammary tumorigenesis in rats. However, the relative contribution to carcinogenesis by calories derived from fat or from carbohydrate are not well established. To determine the relative effects of calories from fat or from carbohydrate, as well as any interaction of dietary fiber on the promotion of 7,12-dimethylbenz[a]anthracene-induced mammary tumors, we fed isocalorically nine diets containing different ratios of fat, carbohydrate, and fiber to female Sprague-Dawley rats treated with 7,12-dimethylbenz[a]anthracene (30/group). Under conditions of isocaloric consumption, at or near ad libitum feeding, calories from dietary fat had approximately twofold greater promoting effect on final body weight and tumor incidence than calories derived from dietary carbohydrate. Dietary fiber had an inhibitory effect on tumor development, but the effect was evident only in the high-fat groups. Logistic regression analysis of tumor incidence gave beta-coefficient estimates for the relative effects of fat, carbohydrate, and fiber of 0.866, 0.189, and -4.281, respectively. Time-to-tumor analysis by the Weibull model indicated beta-estimates of 3.016, 3.324, and 5.825 for dietary fat, carbohydrate, and fiber, respectively, indicating that fat shortens and fiber increases the length of time to tumor. The statistical model derived from these results also indicates a significant synergistic interaction of dietary fat and carbohydrate on final body weight and tumor incidence.
Subject(s)
Dietary Carbohydrates/analysis , Dietary Fats/adverse effects , Dietary Fiber/analysis , Dietary Fiber/therapeutic use , Energy Intake , Mammary Neoplasms, Animal/diet therapy , 9,10-Dimethyl-1,2-benzanthracene , Animals , Carcinogens , Female , Logistic Models , Mammary Neoplasms, Animal/chemically induced , Rats , Rats, Sprague-DawleyABSTRACT
On the basis of reports of rat mammary- and pancreas-tumor models, we hypothesized that an increase in consumption of linoleic acid (LA) would also cause an enhancement in mouse skin-tumor promotion. SEN-CAR mice were placed on diets containing 0.8%, 2.2%, 3.5%, 4.5%, 5.6%, 7.0%, or 8.4% LA, 1 week after initiation with 7,12-dimethylbenz(a)anthracene and 3 weeks before starting promotion with 12-O-tetradecanoylphorbol-13-acetate. An inverse correlation (r = -0.92) was observed between papilloma number and level of LA; however, there was little difference in tumor incidence. A relationship between diet and carcinoma incidence was also found. The fatty acid composition of epidermal phospholipids reflected the dietary LA levels. 12-O-Tetradecanoylphorbol-13-acetate-induced epidermal prostaglandin E2 levels generally decreased with increasing dietary LA. To determine whether this inverse correlation between dietary LA and tumor yield was due to species differences or organ-model differences, a mammary carcinogenesis experiment was performed. SENCAR mice were fed the 0.8%, 4.5%, and 8.4% LA diets. All mice received 6 mg 7,12-dimethylbenz(a)anthracene, administered intragastrically at 1 mg/week. Tumor appearance was delayed in the 0.8% LA diet group, and a positive dose-response relationship between dietary LA and mammary-tumor incidence was observed. These studies suggest that the effect of dietary LA on tumor development is target tissue specific rather than species specific.
Subject(s)
Dietary Fats/adverse effects , Linoleic Acids/adverse effects , Mammary Neoplasms, Experimental/chemically induced , Skin Neoplasms/chemically induced , 9,10-Dimethyl-1,2-benzanthracene , Animals , Dietary Fats/administration & dosage , Dinoprostone/analysis , Fatty Acids/analysis , Fatty Acids/chemistry , Female , Linoleic Acid , Linoleic Acids/administration & dosage , Mice , Skin/chemistry , Tetradecanoylphorbol AcetateABSTRACT
We recently reported (J. Leyton et al., Cancer Res., 51: 907-915, 1991) an inverse correlation between skin tumor number and level of dietary linoleic acid (LA) in SENCAR mice following an initiation-promotion protocol. These results differed from the reported (C. Ip et al., Cancer Res., 45: 1997-2001, 1985) positive correlation between dietary LA and tumor incidence for the rat mammary gland. The goal of the study reported here was to determine whether this dissimilarity was due to organ site or species differences. Female SENCAR mice were fed 1 of 3 15% fat diets containing LA at levels of 0.8, 4.5, and 8.4% before, during, and after intragastric administration of 6 mg (1 mg/week) 7,12-dimethylbenz(a)anthracene. A positive correlation between level of dietary LA and mammary tumor incidence was observed such that for the first 15 weeks, the incidence was greatest in the 8.4% LA diet group, followed by the 4.5% and then the 0.8% LA groups. Distinct dietary effects on latency were also noted in that 15, 12, and 8 weeks after cessation of 7,12-dimethylbenz(a)anthracene were required for a 40% carcinoma incidence in the 0.8, 4.5, and 8.4% LA diet groups, respectively. A histopathological analysis of all tumors revealed that the predominant type was the adenosquamous carcinoma, which comprised 46.6, 54.1, and 77.7% of all mammary tumors for diets containing 0.8, 4.5, and 8.4% LA, respectively. The second most common tumor was the adenocarcinoma type B, which was found with a frequency of 33% in the 0.8% and 4.5% LA diet groups and 22% in the 8.4% LA diet group. These results indicate that SENCAR mice have a short latency period for 7,12-dimethylbenz(a)anthracene-induced mammary tumor development and that rat and mouse mammary tumor development is modified by dietary LA in a similar manner, although in the SENCAR mouse dietary LA did not have a saturating effect. In addition, high dietary LA was found to be associated specifically with an increased incidence of adenosquamous carcinomas but not of other types of mammary tumors.
Subject(s)
Dietary Fats/pharmacology , Mammary Neoplasms, Experimental/pathology , 9,10-Dimethyl-1,2-benzanthracene , Analysis of Variance , Animals , Female , Linoleic Acid , Linoleic Acids/analysis , Mammary Neoplasms, Experimental/chemically induced , Mammary Neoplasms, Experimental/classification , Mice , Mice, Inbred Strains , Time FactorsABSTRACT
An analysis of hepatic aflatoxin B1-DNA adduct (HADA) concentrations in rats and trout demonstrated that the hepatic cancer risk was linearly and quantitatively related in both species. Additionally, the adduct-risk correlation for short-term exposure accurately predicted chronic exposure risk in trout. Although rat and trout liver cells differ substantially in their nuclear DNA content, they appeared to experience similar cancer risks at a given HADA concentration. This may indicate that the liver nuclei of both species contain a similar number of an equivalent protooncogene(s). This analysis also provided a possible basis for the use of adduct measurements for estimating human cancer risk. The minimum human virtual safe dose for aflatoxin was estimated to be 0.264 ng/kg/day on the basis of this approach.