Whole-Genome Sequences of Variants of Bacillus anthracis Sterne and Their Toxin Gene Deletion Mutants.

Here, we report the draft genome sequences of three laboratory variants of Bacillus anthracis Sterne and their double (Δlef Δcya) and triple (Δpag Δlef Δcya) toxin gene deletion derivatives.

[Bionomical aspects of the Squamatoides triviattus (Diptera, Sarcophagidae) under laboratory conditions]. / Aspectos bionômicos de Squamatoides trivittatus (Diptera, Sarcophagidae) sob condições de laboratório.

Experiments with Squamatoides trivitattus were carried out in two different controlled temperatures (16 +/- 1 degree C/50-60% RH and 27 +/- 1 degree C/70-80% RH). The viability of larvae and pupae at 27 degrees C was 89.82% and 92.75% respectively. Larvae did not develop at 16 degrees C. Larval development lasted for 20 +/- 4 hr, 16 +/- 8 hr and 60 +/- 7 hr for the first, second and third instars, respectively, completing a total of 96 +/- 6 hr. The mean pupal period lasted for 15.7 +/- 1.6 days. In longevity tables for the adults, life-expectancy for 50% of the colony submitted to 16 degrees C was of 1.78 weeks for males and 2.42 for females. At 27 degrees C a life-expectancy of 1.15 weeks for males and 0.78 week for females was recorded. The average life-spans for males and females at 16 degrees C were 3.5 +/- 2.0 and 3.8 +/- 2.6 weeks, respectively, and 1.9 +/- 1.2 weeks for both sexes. At 27 degrees C, the longevity recorded was of 2.1 +/- 1.3 weeks for males and 1.7 +/- 1.1 week for females.

Positive thinking in a (too often) negative environment.

With the uncertainty surrounding the future of medicine, we might fear that no one will be drawn to the profession; yet, statistics show that medical school enrollments continue to rise. In this article, we look at the choices, constants, and bottom line of joining the profession.