Subject(s)
Chlormadinone Acetate/pharmacology , Estradiol/metabolism , Mammary Glands, Animal/metabolism , Organ Culture Techniques , Acetates , Animals , Binding Sites , Binding, Competitive/drug effects , Drug Synergism , Estradiol/administration & dosage , Female , Mammary Glands, Animal/drug effects , Rats , Research Design , TritiumSubject(s)
Photic Stimulation , Pineal Gland/metabolism , RNA/biosynthesis , Serotonin/metabolism , Animals , Carbon Isotopes , Cerebral Cortex/metabolism , Chromatography, Thin Layer , Dark Adaptation , Ducks , Male , Organ Culture Techniques , Organ Specificity , Photoreceptor Cells/metabolism , Tritium , Uridine/metabolismABSTRACT
PIP: The action of chlormadinone acetate on the estradiol uptake by rat organ cultures was investigated. Rat hypothalami and uteri tissue were incubated in TC 199 medium for 49 or 72 hours with tritiated estradiol-17beta and unlabeled estradiol or chlormadinone acetate. Chlormadinone acetate added simultaneously to tritiated estradiol inhibited the estradiol uptake (p less than .01) and when added 24 hours previously, the uptake increased (p less than .01) in both uterus and hypothalamus. Unlabeled estradiol also diminished (p less than .01) the estradiol uptake when added simultaneously and increased its uptake when added prior to tritiated estradiol-17beta. These results may indicate an estrogeniclike effect of chlormadinone acetate, perhaps by increasing the binding sites.^ieng
