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Mamm Genome ; 3(2): 112-8, 1992.
Article in English | MEDLINE | ID: mdl-1352156

ABSTRACT

To expand the mink map, we established a new panel consisting of 23 mink-mouse clones. On the basis of statistical criteria (Wijnen et al. 1977; Burgerhout 1978), we developed a computer program for choice of clones of the panel. Assignments of the following mink genes were achieved with the use of the hybrid panel: glyoxalase (GLO), Chromosome (Chr) 1; acetyl acylase (ACY), Chr 5; creatine phosphokinase B (CKBB), Chr 10; alcohol dehydrogenase-2 (subunit B) (ADH2), Chr 8. Using a series of clones carrying rearrangements involving mink Chr 1 and 8, we assigned the gene for ME1 to the short arm of Chr 1 and that for ADH2 to Chr 8, in the region 8p12-p24. Mapping results confirm the ones we previously obtained with a mink-Chinese hamster panel. However, by means of an improved electrophoretic technique, we revised the localization of the gene for purine nucleoside phosphorylase (NP), which has been thought to be on mink Chr 2. It is reassigned to mink Chr 10.


Subject(s)
Chromosome Mapping , Enzymes/genetics , Hybrid Cells , Acetyl-CoA Hydrolase/genetics , Alcohol Dehydrogenase/genetics , Animals , Creatine Kinase/genetics , Isoenzymes/genetics , Lactoylglutathione Lyase/genetics , Malate Dehydrogenase/genetics , Malate Dehydrogenase (NADP+) , Mice , Mink , Pentosyltransferases/genetics
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