ABSTRACT
MR and MS adhesins on 169 strains of Escherichia coli subjected to different cultural conditions were detected. Haemagglutination Test (static settling test in plastic microtiter trays) was used and several species of red blood cells were employed. The results confirm that different media can influence the expression of the adhesins and that using as many species of red blood cells as possible one can detect different adhesins.
Subject(s)
Bacterial Adhesion , Bacterial Proteins/analysis , Escherichia coli/analysis , Adhesins, Escherichia coli , Animals , Culture Media , Escherichia coli/physiology , Hemagglutination Tests , HumansABSTRACT
API 20E, Enterotube II, Sensititre AP60 have been evaluated. Some biochemical tests (lysine, ornithine, VP, H2S, adonitol , arabinose, citrate) have revealed significative differences among three systems. The total correlations about bacterial identifications have been very similar. The repetibility of identifications of strains isolated from clinical specimens has been 98.7% for Enterotube and API 20E, 95.5% for Sensititre . The biochemical tests have revealed repetibility greater than or equal to 94%, citrate repetibility instead has been 91%.