ABSTRACT
The pistachio twig borer, Kermania pistaciella Amsel (Lepidoptera: Tineidae), a key pest of pistachio trees, is a monovoltine pest living inside the feeding tunnel of pistachio twigs for almost 10 months in a year and overwinters there as last instar larvae. In this study, we measured some physiological parameters of overwintering field collected larvae of the pest. There were no changes in trehalose, glucose, and myo-inositol contents, but there were differences in the levels of total simple sugar and glycogen during overwintering. Total sugar content at the beginning of overwintering (October) was at the lowest level (24.13 mg/g body weight) and reached to the highest level (55.22 mg/g fresh body weight) in November whereas glycogen content was at the highest level (44.05 mg/g fresh body weight) in October and decreased to 18.42 mg/g fresh body weight in November. Decrease in lipid content during the overwintering period was not significant. The highest and lowest levels of protein content were recorded in January and February, respectively. Supercooling points (SCP) of the overwintering larvae were stable and low (ranged between -17.80 and -25.10°C) throughout the cold season and no larva survived after SCP determination. The lowest cold hardiness (60 and 0.0% survival following exposure to -10 and -20°C/24 h, respectively) was observed for in November-collected larvae. Overwintering larvae of the pistachio twig borer rely mostly on maintaining the high supercooling capacity throughout the overwintering to avoid freezing of their body fluid.