ABSTRACT
In this work, a pH-sensitive carboxymethylcellulose based bio-nanocomposite hydrogel beads with different content of layered double hydroxides (LDHs) as a nanoparticle was prepared (CMC/LDH(Cu/Al)). EDX spectroscopy was used to confirm the successful composition of LDH(Cu/Al) with CMC and its presence in the hydrogel matrix. The prepared CMC/LDH(Cu/Al) bio-nanocomposite hydrogel bead characterized by XRD, FT-IR, and SEM analysis. The swelling results showed the pH-sensitive properties for all of the prepared CMC/LDH(Cu/Al). Amoxicillin (AMX) as a model of the antibiotic drug was selected to study the ability of the prepared systems as an oral drug delivery vehicle. The obtained results showed that the CMC/LDH(Cu/Al 7.5) bio-nanocomposite hydrogel bead has a good performance compared to the other prepared bio-nanocomposites. MTT assay approved the safety of this bio-nanocomposite hydrogel bead against HUVEC cells. Consistent with the obtained results, the prepared hydrogel beads could be potentially proposed as an efficient safe drug carrier for AMX oral delivery.
Subject(s)
Amoxicillin/chemistry , Amoxicillin/pharmacology , Bacterial Infections/drug therapy , Carboxymethylcellulose Sodium/chemistry , Colon/microbiology , Hydrogels/chemistry , Hydroxides/chemistry , Amoxicillin/therapeutic use , Colon/drug effects , Drug Carriers/chemistry , Drug Carriers/toxicity , Human Umbilical Vein Endothelial Cells , Humans , Microspheres , Nanocomposites/chemistry , SafetyABSTRACT
Due to the decrease of the drugs side effects in controlled drug delivery systems, today's many research has been focused on designing the new controlled drug delivery systems. The main aim of the present work was the improving of the layered double hydroxide (LDH) properties as a drug delivery system through the encapsulation with carboxymethyl cellulose (CMC). In the first section, 5-fluorouracil (5-Fu) as a colon anticancer drug was loaded in LDH(Zn/Al) (about 87%). The FT-IR, SEM, and XRD analysis were used to probe the successful synthesis of LDH(Zn/Al) and 5-Fu loading in it. In the following, the nanohybrid encapsulated with CMC, through the crosslinking of the LDH(Zn/Al)-5-Fu and CMC mixture with Fe(III) as a physical crosslinker and CMC/LDH(Zn/Al)-5-Fu hydrogel beads were formed. From comparing of the 5-Fu release from the LDH(Zn/Al)-5-Fu and CMC/LDH(Zn/Al)-5-Fu hydrogel beads, it was found that the use of CMC as a capsule induce the controlled and sustained drug release behavior to the system. MTT assay approved the biocompatibility of the CMC/LDH(Zn/Al)-5-Fu hydrogel beads. With considering the swelling, drug loading, drug-releasing, and MTT assay results, the prepared CMC/LDH(Zn/Al)-5-Fu hydrogel beads system could be proposed as a potentially safe oral delivery system for colon cancer therapy.