Subject(s)
Leukemia, Megakaryoblastic, Acute/genetics , Oncogene Proteins, Fusion/genetics , Transcription, Genetic , Base Sequence , Child , Down Syndrome/genetics , Humans , Leukemia, Megakaryoblastic, Acute/immunology , Male , Receptors, Antigen, T-Cell, gamma-delta/genetics , Reverse Transcriptase Polymerase Chain ReactionSubject(s)
Apoptosis/genetics , Core Binding Factor Alpha 2 Subunit/genetics , Oncogene Proteins, Fusion/genetics , Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/genetics , Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics , Proto-Oncogene Proteins c-bcl-2/genetics , Amino Acid Sequence , Child , Humans , Molecular Sequence Data , Transcription, GeneticABSTRACT
Monocytic cell lines (HL-60 and THP-1) were infected with viable Mycobacterium leprae. Levels of human hsp60 were estimated by Western blot (immunoblot) assay and a sandwich enzyme-linked immunosorbent assay. The results showed that infection of both of the cell lines induced the synthesis of human hsp60, which may be of significance in relation to autoimmune manifestations associated with mycobacterial infections.
Subject(s)
Chaperonin 60/biosynthesis , Monocytes/metabolism , Mycobacterium leprae/physiology , Cell Line , Cytokines/pharmacology , HumansABSTRACT
A total of 220 untreated leprosy patients who underwent parallel skin and nerve biopsies are included in this study, which is intended to evaluate the extent of previously reported differences in bacillary load between skin and nerve lesions in leprosy and to describe the response of peripheral blood lymphocytes to Mycobacterium leprae antigens in such patients. In 161 patients out of the 220, the skin and nerve biopsies were diagnostic for leprosy. When patients were grouped according their skin and nerve lesions, the 3 groups observed were (1) paucibacillary skin and nerve lesions; (2) multibacillary skin and nerve lesions, and (3) paucibacillary skin and multibacillary nerve lesions. There was no observation of a group of patients with multibacillary skin and paucibacillary nerve lesions. In all patients with multibacillary nerve lesions, regardless of the type of skin lesions, a low response of peripheral blood lymphocytes to M. leprae was consistently noted. These results suggest that the bacillary load in the nerve is certainly one of the factors determining the immunological spectrum observed in leprosy.
