Bacillus subtilis rec Assay for Quantification of Aflatoxins.

The Bacillus subtilis 1791 rec- assay was used to quantify genotoxic mycotoxins. This assay is based on detection of mycotoxin-produced DNA alterations arising from recombinational deficiency in rec- cells. Aflatoxin B1 showed a linear dose-response relationship when the inhibition halo was taken as a parameter for the evaluation procedure. Assays carried out with or without hepatic microsomal activation exhibited a similar response.