ABSTRACT
We studied DNA-damaging effects of dental bleaching systems containing hydrogen peroxide and/or carbamide peroxide by the "comet assay" (alkaline version). Dental bleaching systems in a hydrogen peroxide concentration range from 0.03 to 30 mM produced a genotoxic effect on isolated HeLa cells in vitro comparable with the effects of pharmacopoeial hydrogen peroxide or urea peroxide. Catalase protected the cells against products containing hydrogen peroxide and had no effect on the genotoxicity of samples containing carbamide peroxide.
Subject(s)
Bleaching Agents/pharmacology , DNA Damage/drug effects , Carbamide Peroxide , Catalase/metabolism , Comet Assay , Humans , Hydrogen Peroxide/pharmacology , Peroxides/pharmacology , Urea/analogs & derivatives , Urea/pharmacologyABSTRACT
We studied umbilical cord blood mesenchymal stem cells and compared mesenchymal stem cells derived from umbilical cord blood, adipose tissue, and skin. Umbilical cord blood mesenchymal stem cells were characterized morphologically, cytofluorometrically, and by their differentiation potential. Umbilical cord blood mesenchymal stem cells did not differ from cells isolated from adipose tissue and skin by the main parameters (by morphology, expression of surface markers, and differentiation potential). A specific feature of umbilical cord blood mesenchymal stem cells is their low count per volume of the initial material and very low proliferative activity.
Subject(s)
Fetal Blood/cytology , Mesenchymal Stem Cells/cytology , Adipocytes/cytology , Adipose Tissue/cytology , Adult , Cell Differentiation , Cell Proliferation , Female , Humans , Osteoblasts/cytology , Skin/cytologyABSTRACT
We compared differentiation potential of mesenchymal stem cells originating from human bone marrow, fatty tissue, thymus, placenta, and skin. The cells were characterized by differentiation into adipocytes and osteoblasts. Mesenchymal stem cells from different sources exhibited different differentiation potential, manifesting by the rate of differentiation and percentage of differentiated cells. Presumably, differentiation of mesenchymal stem cells derived from different tissues can differ due to the presence of progenitor cells of different types.
Subject(s)
Cell Differentiation , Mesenchymal Stem Cells/cytology , Adipose Tissue/cytology , Adolescent , Adult , Bone Marrow Cells/cytology , Bone Marrow Cells/physiology , Female , Humans , Male , Mesenchymal Stem Cells/physiology , Middle Aged , Placenta/cytology , Skin/cytology , Thymus Gland/cytologyABSTRACT
We studied mesenchymal stem cells from human bone marrow, adipose tissue, skin, placenta, and thymus. Morphological study and cytofluorometrical analysis by the main marker genes (CD10, CD13, CD31, CD44, CD90, CD105) were carried out. Mesemchymal stem cells of the studied tissues during isolation and culturing were morphologically similar and did not differ by the expression of the main marker genes.
