Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 10 de 10
Filter
Add more filters










Publication year range
1.
Front Cardiovasc Med ; 9: 980628, 2022.
Article in English | MEDLINE | ID: mdl-36035957

ABSTRACT

Background: Adequate blood flow into coronary micro-arteries is essential for myocardial function. Here we assess the mechanisms responsible for amplifying blood flow into myogenically-contracting human and porcine intramyocardial micro-arteries ex vivo using endothelium-dependent and -independent vasodilators. Methods: Human and porcine atrial and ventricular small intramyocardial coronary arteries (IMCAs) were studied with pressure myography and imaged using confocal microscopy and serial section/3-D reconstruction EM. Results: 3D rendered ultrastructure images of human right atrial (RA-) IMCAs revealed extensive homo-and hetero-cellular contacts, including to longitudinally-arranged smooth muscle cells (l-SMCs) found between the endothelial cells (ECs) and radially-arranged medial SMCs (r-SMCs). Local and conducted vasodilatation followed focal application of bradykinin in both human and porcine RA-IMCAs, and relied on hyperpolarization of SMCs, but not nitric oxide. Bradykinin initiated asynchronous oscillations in endothelial cell Ca2+ in pressurized RA-IMCAs and, as previously shown in human RA-IMCAs, hyperpolarized porcine arteries. Immunolabelling showed small- and intermediate-conductance Ca2+-activated K+ channels (KCa) present in the endothelium of both species, and concentration-dependent vasodilation to bradykinin followed activation of these KCa channels. Extensive electrical coupling was demonstrated between r-SMCs and l-SMCs, providing an additional pathway to facilitate the well-established myoendothelial coupling. Conducted dilation was still evident in a human RA-IMCA with poor myogenic tone, and heterocellular contacts were visible in the 3D reconstructed artery. Hyperpolarization and conducted vasodilation was also observed to adenosine which, in contrast to bradykinin, was sensitive to combined block of ATP-sensitive (KATP) and inwardly rectifying (KIR) K+ channels. Conclusions: These data extend our understanding of the mechanisms that coordinate human coronary microvascular blood flow and the mechanistic overlap with porcine IMCAs. The unusual presence of l-SMCs provides an additional pathway for rapid intercellular signaling between cells of the coronary artery wall. Local and conducted vasodilation follow hyperpolarization of the ECs or SMCs, and contact-coupling between l-SMCs and r-SMCs likely facilitates this vasodilation.

2.
Cardiovasc Res ; 118(8): 1978-1992, 2022 06 29.
Article in English | MEDLINE | ID: mdl-34173824

ABSTRACT

AIMS: Coronary microvascular smooth muscle cells (SMCs) respond to luminal pressure by developing myogenic tone (MT), a process integral to the regulation of microvascular perfusion. The cellular mechanisms underlying poor myogenic reactivity in patients with heart valve disease are unknown and form the focus of this study. METHODS AND RESULTS: Intramyocardial coronary micro-arteries (IMCAs) isolated from human and pig right atrial (RA) appendage and left ventricular (LV) biopsies were studied using pressure myography combined with confocal microscopy. All RA- and LV-IMCAs from organ donors and pigs developed circa 25% MT. In contrast, 44% of human RA-IMCAs from 88 patients with heart valve disease had poor (<10%) MT yet retained cell viability and an ability to raise cytoplasmic Ca2+ in response to vasoconstrictor agents. Comparing across human heart chambers and species, we found that based on patient medical history and six tests, the strongest predictor of poor MT in IMCAs was increased expression of the synthetic marker caldesmon relative to the contractile marker SM-myosin heavy chain. In addition, high resolution imaging revealed a distinct layer of longitudinally aligned SMCs between ECs and radial SMCs, and we show poor MT was associated with disruptions in these cellular alignments. CONCLUSION: These data demonstrate the first use of atrial and ventricular biopsies from patients and pigs to reveal that impaired coronary MT reflects a switch of viable SMCs towards a synthetic phenotype, rather than a loss of SMC viability. These arteries represent a model for further studies of coronary microvascular contractile dysfunction.


Subject(s)
Heart Valve Diseases , Muscle, Smooth, Vascular , Animals , Coronary Vessels/pathology , Heart Valve Diseases/metabolism , Humans , Muscle Contraction , Muscle, Smooth, Vascular/metabolism , Myocytes, Smooth Muscle/metabolism , Swine
3.
Br J Hosp Med (Lond) ; 80(1): 40-45, 2019 Jan 02.
Article in English | MEDLINE | ID: mdl-30592667

ABSTRACT

Heart failure is an increasingly prevalent chronic condition which causes substantial morbidity and mortality, placing an increasing economic burden on health care. Hospitalizations as a result of heart failure are projected to increase considerably over the next two decades. A robust restructuring of existing heart failure treatment models in the UK is needed to enable an integrated seamless transition of care across the community, primary care and hospital networks. This has to be achieved with the patient as a partner in health care as a part of a multidisciplinary approach. This uses innovative strategies such as ambulatory treatment (including intravenous diuretics, remote and telemonitoring) as well as shifting heart failure treatment to the community and to patients' homes. This article analyses the existing evidence for ambulatory management of acute decompensated heart failure and looks at future strategies for restructuring care.


Subject(s)
Ambulatory Care/methods , Diuretics/therapeutic use , Heart Failure/therapy , Acute Disease , Administration, Intravenous , Chronic Disease , Disease Management , Early Diagnosis , Early Medical Intervention , Hospitalization , Humans , Symptom Flare Up
4.
Proc Natl Acad Sci U S A ; 109(44): 18174-9, 2012 Oct 30.
Article in English | MEDLINE | ID: mdl-23071308

ABSTRACT

Endothelial cell (EC) Ca(2+)-activated K channels (SK(Ca) and IK(Ca) channels) generate hyperpolarization that passes to the adjacent smooth muscle cells causing vasodilation. IK(Ca) channels focused within EC projections toward the smooth muscle cells are activated by spontaneous Ca(2+) events (Ca(2+) puffs/pulsars). We now show that transient receptor potential, vanilloid 4 channels (TRPV4 channels) also cluster within this microdomain and are selectively activated at low intravascular pressure. In arterioles pressurized to 80 mmHg, ECs generated low-frequency (~2 min(-1)) inositol 1,4,5-trisphosphate receptor-based Ca(2+) events. Decreasing intraluminal pressure below 50 mmHg increased the frequency of EC Ca(2+) events twofold to threefold, an effect blocked with the TRPV4 antagonist RN1734. These discrete events represent both TRPV4-sparklet- and nonsparklet-evoked Ca(2+) increases, which on occasion led to intracellular Ca(2+) waves. The concurrent vasodilation associated with increases in Ca(2+) event frequency was inhibited, and basal myogenic tone was increased, by either RN1734 or TRAM-34 (IK(Ca) channel blocker), but not by apamin (SK(Ca) channel blocker). These data show that intraluminal pressure influences an endothelial microdomain inversely to alter Ca(2+) event frequency; at low pressures the consequence is activation of EC IK(Ca) channels and vasodilation, reducing the myogenic tone that underpins tissue blood-flow autoregulation.


Subject(s)
Arterioles/metabolism , Calcium/metabolism , Endothelium, Vascular/metabolism , Potassium Channels/metabolism , Animals , Arterioles/physiology , Endothelium, Vascular/physiology , Muscle Tonus , Potassium Channel Blockers/pharmacology , Pyrazoles/pharmacology , Rats , Sulfonamides/pharmacology , Vasodilation
5.
Vascul Pharmacol ; 56(3-4): 115-21, 2012.
Article in English | MEDLINE | ID: mdl-22155161

ABSTRACT

We hypothesized that under high glucose conditions, activation of the hexosamine pathway leads to impaired nitric oxide (NO)-dependent arteriolar dilation. Skeletal muscle arterioles (diameter: ~160µm) isolated from male Wistar rats were exposed to normal glucose (NG, 5.5mmol/L) or high glucose concentrations (HG, 30mmol/L, for 2h) and agonist-induced diameter changes were measured with videomicroscopy. Western blots were performed to identify the vascular levels of protein O-linked-N-acetyl-glucosamine (O-GlcNAc) and phosphorylated endothelial NO synthase (eNOS). In arterioles exposed to HG, dilations to histamine were abolished compared to those exposed to NG (max: -6±6% and 69±9%, respectively), while acetylcholine-induced responses were not affected. Inhibition of NO synthesis with N(G)-nitro-l-arginine methyl ester (L-NAME) reduced histamine-induced dilations in NG arterioles, but it had no effect on microvessels exposed to HG. Dilations to the NO donor, sodium nitroprusside and constrictions to norepinephrine and serotonin were similar in the two groups. In the presence of the inhibitor of hexosamine pathway, azaserine, histamine-induced dilations were significantly augmented in arterioles exposed to HG (max: 67±2%). Moreover, exposure of vessels to glucosamine (5mmol/L, for 2h) resulted in reduced histamine-induced arteriolar dilations (max: 26±3%). The level of protein O-GlcNAcylation was increased, whereas the P-eNOS (Ser-1177) was decreased in HG exposed vessels. These findings indicate that a high concentration of glucose may lead to glucosamine formation, which impairs histamine-induced, NO-mediated arteriolar dilations. We propose that interfering with the hexosamine pathway may prevent microvascular complications in diabetes.


Subject(s)
Acetylglucosamine/metabolism , Arterioles/metabolism , Hexosamines/metabolism , Hyperglycemia/metabolism , Nitric Oxide/metabolism , Protein Processing, Post-Translational , Vasodilation , Aminoacylation/drug effects , Animals , Arterioles/drug effects , Arterioles/physiopathology , Diabetic Angiopathies/prevention & control , Enzyme Activation/drug effects , Enzyme Inhibitors/pharmacology , Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)/antagonists & inhibitors , Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)/metabolism , Hyperglycemia/physiopathology , In Vitro Techniques , Male , Muscle, Skeletal/blood supply , Nitric Oxide Synthase Type III/antagonists & inhibitors , Nitric Oxide Synthase Type III/metabolism , Phosphorylation/drug effects , Protein Processing, Post-Translational/drug effects , Rats , Rats, Wistar , Vasodilation/drug effects , Vasodilator Agents/pharmacology
6.
Cardiovasc Res ; 92(2): 199-208, 2011 Nov 01.
Article in English | MEDLINE | ID: mdl-21690174

ABSTRACT

AIMS: Agonists that evoke smooth muscle cell hyperpolarization have the potential to stimulate both local and conducted dilation. We investigated whether the endothelium-dependent vasodilators acetylcholine (ACh) and SLIGRL stimulated conducted dilation and whether this was altered by deficiency in apolipoprotein E (ApoE(-/-)). METHODS AND RESULTS: Isolated mesenteric arteries were cannulated, pressurized, and precontracted with phenylephrine. Agonists were either added to the bath to study local dilation or were restricted to one end of arteries to study conducted dilation. An enhanced sensitivity to both ACh and SLIGRL was observed in mesenteric arteries from ApoE(-/-) mice compared with wild-type controls. Inhibition of nitric oxide (NO) synthase blocked ACh responses, but had no effect on maximum dilation to SLIGRL. SLIGRL increased endothelial cell Ca(2+), hyperpolarized smooth muscle cells, and fully dilated arteries. The NO-independent dilation to SLIGRL was blocked with high [KCl] or Ca(2+)-activated K(+)-channel blockers. The hyperpolarization and dilation to SLIGRL passed through the artery to at least 2.5 mm upstream. The conducted dilation was not affected by a deficit in ApoE and could also be stimulated by ACh, suggesting NO itself could stimulate conducted dilation. CONCLUSION: In small mesenteric arteries of ApoE(-/-) mice, NO-independent dilation is enhanced. Since both NO-dependent and -independent pathways can stimulate local and conducted dilation, the potential for reducing vascular resistance is improved in these vessels.


Subject(s)
Aortic Diseases/metabolism , Apolipoproteins E/deficiency , Atherosclerosis/metabolism , Endothelium, Vascular/metabolism , Mesenteric Arteries/metabolism , Potassium Channels/metabolism , Vasodilation , Acetylcholine/pharmacology , Age Factors , Animals , Aortic Diseases/genetics , Aortic Diseases/physiopathology , Apolipoproteins E/genetics , Atherosclerosis/genetics , Atherosclerosis/physiopathology , Calcium/metabolism , Disease Models, Animal , Dose-Response Relationship, Drug , Endothelium, Vascular/drug effects , Endothelium, Vascular/physiopathology , Enzyme Inhibitors/pharmacology , Mesenteric Arteries/drug effects , Mesenteric Arteries/physiopathology , Mice , Mice, Inbred C57BL , Mice, Knockout , Nitric Oxide/metabolism , Nitric Oxide Synthase/antagonists & inhibitors , Nitric Oxide Synthase/metabolism , Oligopeptides/pharmacology , Potassium Channel Blockers/pharmacology , Potassium Channels/drug effects , Vasodilation/drug effects , Vasodilator Agents/pharmacology
7.
Microcirculation ; 18(6): 487-500, 2011 Aug.
Article in English | MEDLINE | ID: mdl-21535295

ABSTRACT

OBJECTIVE: To assess the influence of blocking smooth muscle large conductance Ca(2+) -activated K+ channels and voltage-gated K+ channels on the conducted dilation to ACh and isoproterenol. MATERIALS AND METHODS: Rat mesenteric arteries were isolated with a bifurcation, triple-cannulated, pressurized and imaged using confocal microscopy. Phenylephrine was added to the superfusate to generate tone, and agonists perfused into a sidebranch to evoke local dilation and subsequent conducted dilation into the feed artery. RESULTS: Both ACh- and isoproterenol-stimulated local and conducted dilation with similar magnitudes of decay with distance along the feed artery (2000µm: ∼15% maximum dilation). The gap junction uncoupler carbenoxolone prevented both conducted dilation and intercellular spread of dye through gap junctions. IbTx, TEA or 4-AP, blockers of large conductance Ca(2+) -activated K+ channels and voltage-gated K+ channels, did not affect conducted dilation to either agonist. A combination of either IbTx or TEA with 4-AP markedly improved the extent of conducted dilation to both agonists (2000µm: >50% maximum dilation). The enhanced conducted dilation was reflected in the hyperpolarization to ACh (2000µm: Control, 4±1 mV, n = 3; TEA with 4-AP, 14±3mV, n=4), and was dependent on the endothelium. CONCLUSIONS: These data show that activated BK(Ca) and K(V) -channels serve to reduce the effectiveness of conducted dilation.


Subject(s)
Mesenteric Arteries/physiology , Muscle, Smooth, Vascular/metabolism , Potassium Channels, Calcium-Activated/metabolism , Potassium Channels, Voltage-Gated/metabolism , Vasodilation/physiology , Acetylcholine/pharmacology , Adrenergic beta-Agonists/pharmacology , Animals , Gap Junctions/metabolism , Isoproterenol/pharmacology , Male , Potassium Channel Blockers/pharmacology , Potassium Channels, Calcium-Activated/antagonists & inhibitors , Potassium Channels, Voltage-Gated/antagonists & inhibitors , Rats , Rats, Wistar , Vasodilation/drug effects , Vasodilator Agents/pharmacology
8.
Am J Physiol Heart Circ Physiol ; 300(3): H777-83, 2011 Mar.
Article in English | MEDLINE | ID: mdl-21217072

ABSTRACT

Arginase 1, via competing with nitric oxide (NO) synthase for the substrate L-arginine, may interfere with NO-mediated vascular responses. We tested the hypothesis that arginase 1 contributes to coronary vasomotor dysfunction in patients with diabetes mellitus (DM). Coronary arterioles were dissected from the right atrial appendages of 41 consecutive patients with or without DM (the 2 groups suffered from similar comorbidities), and agonist-induced changes in diameter were measured with videomicroscopy. We found that the endothelium-dependent agonist ACh elicited a diminished vasodilation and caused constriction to the highest ACh concentration (0.1 µM) with a similar magnitude in patients with (18 ± 8%) and without (17 ± 9%) DM. Responses to ACh were not significantly affected by the inhibition of NO synthesis with N(G)-nitro-L-arginine methyl ester in either group. The NO donor sodium nitroprusside-dependent dilations were not different in patients with or without DM. Interestingly, we found that the presence of N(G)-hydroxy-L-arginine (10 µM), a selective inhibitor of arginase or application of L-arginine (3 mM), restored ACh-induced coronary dilations only in patients with DM (to 47 ± 6% and to 40 ± 19%, respectively) but not in subjects without DM. Correspondingly, the protein expression of arginase 1 was increased in coronary arterioles of patients with DM compared with subjects without diabetes. Moreover, using immunocytochemistry, we detected an abundant immunostaining of arginase 1 in coronary endothelial cells of patients with DM, which was colocalized with NO synthase. Collectively, we provided evidence for a distinct upregulation of arginase 1 in coronary arterioles of patients with DM, which contributes to a reduced NO production and consequently diminished vasodilation.


Subject(s)
Arginase/metabolism , Coronary Vessels/enzymology , Diabetes Mellitus, Type 1/enzymology , Diabetes Mellitus, Type 2/enzymology , Vasodilation , Acetylcholine/pharmacology , Aged , Arginine/analogs & derivatives , Arginine/pharmacology , Arterioles/drug effects , Coronary Vessels/cytology , Diabetes Mellitus, Type 1/drug therapy , Diabetes Mellitus, Type 1/physiopathology , Diabetes Mellitus, Type 2/drug therapy , Diabetes Mellitus, Type 2/physiopathology , Female , Humans , Hypoglycemic Agents/therapeutic use , Male , Middle Aged , NG-Nitroarginine Methyl Ester/pharmacology , Nitroprusside/pharmacology , Up-Regulation , Vasodilator Agents/pharmacology
9.
Cardiovasc Res ; 87(4): 732-9, 2010 Sep 01.
Article in English | MEDLINE | ID: mdl-20299334

ABSTRACT

AIMS: Caveolin-1 (Cav-1) interacts with large conductance Ca(2+)-activated potassium channels (BKCa) and likely exerts a negative regulatory effect on the channel activity. We investigated the role of Cav-1 in modulating BK(Ca) channel-mediated, endothelium-derived hyperpolarizing factor (EDHF)-dependent arteriolar dilation in normal condition and in an experimental model of obesity. METHODS AND RESULTS: In isolated, pressurized (80 mmHg) gracilis muscle arterioles (approximately 100 microm) of Cav-1 knockout mice, acetylcholine (ACh)-induced, EDHF-mediated dilations were enhanced and were significantly reduced by the BK(Ca) channel inhibitor, iberiotoxin (IBTX), whereas IBTX had no effect on EDHF-mediated dilations in the wild-type mice. Dilations to the selective BK(Ca) channel opener, NS-1619 were augmented in the Cav-1 knockout mice. In high-fat diet-treated, obese rats ACh-induced coronary arteriolar dilations were preserved, whereas IBTX-sensitive, ACh-induced and also NS-1619-evoked vasodilations were augmented when compared with lean animals. In coronary arterioles of obese rats a reduced protein expression of Cav-1 was detected by western immunoblotting and immunohistochemistry. Moreover, in coronary arterioles of lean rats, disruption of caveolae with methyl-beta-cyclodextrin augmented IBTX-sensitive, ACh-induced, and also NS-1619-evoked dilations. CONCLUSION: Thus, under normal conditions, Cav-1 limits the contribution of the BK(Ca) channel to EDHF-mediated arteriolar dilation. In obesity, a reduced expression of Cav-1 leads to greater contribution of the BK(Ca) channel to EDHF-mediated response, which seems essential for maintained coronary dilation.


Subject(s)
Biological Factors/metabolism , Caveolin 1/metabolism , Coronary Vessels/metabolism , Large-Conductance Calcium-Activated Potassium Channels/metabolism , Muscle, Skeletal/blood supply , Obesity/metabolism , Vasodilation , Animals , Arterioles/metabolism , Arterioles/physiopathology , Blotting, Western , Caveolin 1/deficiency , Caveolin 1/genetics , Coronary Vessels/drug effects , Coronary Vessels/physiopathology , Dietary Fats , Disease Models, Animal , Dose-Response Relationship, Drug , Immunohistochemistry , Mice , Mice, Knockout , Obesity/genetics , Obesity/physiopathology , Potassium Channel Blockers/pharmacology , Rats , Rats, Wistar , Vasodilation/drug effects , Vasodilator Agents/pharmacology
10.
Pharmacol Rep ; 61(1): 99-104, 2009.
Article in English | MEDLINE | ID: mdl-19307697

ABSTRACT

Type 2 diabetes mellitus is associated with clustering of cardiovascular risk factors that may greatly increase individuals' risk of developing coronary artery disease. Type 2 diabetes is believed to impair coronary function. However, its impact on the vasomotor function of coronary resistance vessels in humans is still debated. Reduced, preserved or even augmented dilations of coronary arterioles have been reported in subjects with type 2 diabetes. Interestingly, recent studies have suggested that reactive oxygen species (ROS), particularly hydrogen peroxide, may compensate for the loss of the vasodilatory function of coronary microvessels during disease development. Recent interventional clinical trials have yielded largely negative results, and there has even been some suggestion of harm caused by attempts to reduce ROS. Thus, it is possible that interference with ROS-related signaling might paradoxically temper the function of coronary microvessels, predisposing patients to myocardial ischemia. In this review, we aim to highlight current findings supporting a potential role for ROS in preserving coronary arteriolar dilation in type 2 diabetes mellitus.


Subject(s)
Coronary Vessels/physiopathology , Diabetes Mellitus, Type 2/physiopathology , Reactive Oxygen Species/metabolism , Animals , Clinical Trials as Topic , Coronary Artery Disease/etiology , Coronary Artery Disease/physiopathology , Coronary Vessels/metabolism , Diabetes Mellitus, Type 2/complications , Humans , Hydrogen Peroxide/metabolism , Microvessels/metabolism , Oxidative Stress , Risk Factors , Signal Transduction , Vasodilation
SELECTION OF CITATIONS
SEARCH DETAIL
...