Dose-dependent in vitro inhibition of rabbit corneal matrix metalloproteinases by an extract of Pothomorphe umbellata after alkali injury

The in vitro ability of Pothomorphe umbellata ethanolic crude extract to inhibit matrix metalloproteinase (MMP) in normal cornea and in cornea after alkali injury was demonstrated. Corneas of albino rabbits were injured with 1 N NaOH for 20 s. After 48 h the corneas were excised, homogenized and analyzed for MMP-9 (92 kDa), pro-MMP-2 (72 kDa) and MMP-2 (67 kDa) activity by gelatin zymography. The activity was also measured in untreated corneas. After electrophoresis of 20 æg protein, gels were incubated with 50, 100, or 250 µg/mL lyophilized hydroethanolic (1:1) root crude extract of P. umbellata standardized for 4-nerolidylcatechol (7.09 percent). The activity of the enzymes was compared with that of untreated gel. At 48 h after injury, the activity of all MMPs was increased compared with untreated eyes. When the gels were incubated with P. umbellata extract the activity of MMP-2, pro-MMP-2 and MMP-9 decreased in a dose-dependent manner. MMP-9 activity decreased by approximately 50 percent after incubation with 50 µg/mL and was completely abolished at 100 and 250 µg/mL of the extract. After incubation with 50 µg/mL the activity of pro-MMP-2 and MMP-2 also decreased by 50 percent. The activity of pro-MMP-2 was almost completely abolished after incubation with 250 µg/mL of the extract. For MMP-2 the incubation with 100 or 250 µg/mL of the extract of P. umbellata promoted a 10-fold decrease in activity. In conclusion, P. umbellata root crude extract can be useful as an alternative therapy to control MMP activity after corneal injury.

Lack of evidence for superantigen activity of Toxoplasma gondii towards human T cells

Toxoplasma gondii is an obligatory intracellular parasite whose life cycle may include man as an intermediate host. More than 500 million people are infected with this parasite worldwide. It has been previously reported that T. gondii contains a superantigen activity. The purpose of the present study was to determine if the putative superantigen activity of T. gondii would manifest towards human T cells. Peripheral blood mononuclear cells (PBMC) from individuals with no previous contact with the parasite were evaluated for proliferation as well as specific Vá expansion after exposure to Toxoplasma antigens. Likewise, PBMC from individuals with the congenital infection were evaluated for putative Vá family deletions in their T cell repertoire. We also evaluated, over a period of one year, the PBMC proliferation pattern in response to Toxoplasma antigens in patients with recently acquired infection. Some degree of proliferation in response to T. gondii was observed in the PBMC from individuals never exposed to the parasite, accompanied by specific Vá expansion, suggesting a superantigen effect. However, we found no specific deletion of Vá (or Valpha) families in the blood of congenitally infected individuals. Furthermore, PBMC from recently infected individuals followed up over a period of one year did not present a reduction of the Vá families that were originally expanded in response to the parasite antigens. Taken together, our data suggest that T. gondii does not have a strong superantigen activity on human T cells

Treatment of cytomegalovirus retinitis with an intraocular sustained-release ganciclovir implant

The objective of this prospective study was to evaluate the efficacy and complications of the use of an intraocular sustained-release ganciclovir implant for the treatment of active cytomegalovirus (CMV) retinitis in AIDS patients. Thirty-nine eyes of 26 patients were submitted to ocular surgery. All patients underwent complete ocular examination before and after surgery. The surgical procedure was always done under local anesthesia using the same technique. The mean time for the surgical procedure was 20 min (range, 15 to 30 min). The average follow-up period was 3.7 months. Of all patient, only 4 presented recurrence of retinitis after 8, 8, 9 and 2 months, respectively. Three of them received a successful second implant. All 39 eyes of the 26 patients presented healing of retinitis as shown by clinical improvement evaluated by indirect binocular ophthalmoscopy and retinography. Retinitis healed within a period of 4 to 6 weeks in all patients, with clinical regression signs from the third week on. Six (15.4 percent) eyes developed retinal detachment. None of the patients developed CMV retinitis in the contralateral eye. The intraocular implant proved to be effective in controlling the progression of retinitis for a period of up to 8 months even in patients for whom systemic therapy with either ganciclovir or foscarnet or both had failed. The intraocular sustained-release ganciclovir implant proved to be a safe new procedure for the treatment of CMV retinitis, avoiding the systemic side effects caused by the intravenous medications and improving the quality of life of the patients.

Cytomegalovirus antigenemia in acquired immunodeficiency syndrome patients with untreated cytomegalovirus retinitis.

PURPOSE: To determine the frequency of cytomegalovirus (CMV) viremia in patients with acquired immunodeficiency syndrome (AIDS) and untreated CMV retinitis using conventional cell culture isolation and the sensitive CMV antigenemia assay. METHODS: We examined 24 AIDS patients with ophthalmologic diagnosis of untreated CMV retinitis and 24 AIDS patients without present or past retinitis (control patients) from three medical centers between September 1992 and March 1994. Cytomegalovirus antigenemia was detected by an indirect peroxidase staining in 300,000 cytocentrifuged neutrophils, using a mixture of murine monoclonal antibodies directed against the pp65 lower matrix protein of CMV. RESULTS: Positive antigenemia was demonstrated in eight (33.3%) of the 24 retinitis patients and in none of the 24 control patients (P < .001). Only two of the eight antigenemia-positive patients had a concurrent positive CMV isolation from blood leukocytes by conventional cell culture assay. CONCLUSIONS: These results emphasize the risk of extraocular disease in AIDS patients with CMV retinitis because the virus is often present in peripheral blood leukocytes. The CMV antigenemia assay may be a simple and rapid means of identifying those patients with unilateral retinitis at highest risk of developing CMV retinitis of the fellow eye or of visceral CMV disease if intravitreal injections or implants are used as sole treatment for CMV retinitis.

Intrastromal corneal ring: 12-month sighted myopic eyes.

BACKGROUND: To evaluate the efficacy, predictability, and stability of the refractive effect produced by the Intrastromal Corneal Ring (ICR), the authors completed a 12-month study on 10 myopic eyes. METHODS: Ten patients with preoperative refractive errors ranging from -2.63 diopters (D) to -4.25 D (mean, -3.30 D) participated in the study. The attempted correction was -2.50 D for a 7.0-mm diameter, 0.3-mm thick ICR. One eye of each patient received an ICR. RESULTS: The average change in spherical equivalent at 12 months was -2.25 D (SD 0.54 D; range, -1.62 to -3.25 D). All patients maintained a spectacle-corrected visual acuity of 20/20 or better during the 12-month study period, with the exception of patient no. 6 who saw 20/30 at the 6-month examination. Her spectacle-corrected visual acuity returned to 20/20 a few days after the ICR was explanted and remained stable throughout the study. Uncorrected visual acuity had improved to 20/40 or better in all patients on postoperative day 1 and remained in this range for the 9 eyes (90%) during the 12 months of follow up. At postoperative month 12, 9 of 9 eyes (100%) had an uncorrected visual acuity of 20/40 or better with 3 of 9 eyes (33%) seeing 20/20 or better. The remaining patient, no. 6, experienced a tear in Descemet's membrane during the procedure and required explantation of the ring after 6 months due to induced astigmatism and deterioration of uncorrected visual acuity. Two patients developed infiltrates that resolved with the use of antibiotics. The most common postoperative ocular findings were peripheral corneal haze in all eyes that diminished over time, minute lamellar channel deposits (7 of 10 eyes, 70%), deep stromal neovascularization (5 of 10 eyes, 50%), and pannus (5 of 10 eyes, 50%). CONCLUSION: This preliminary study shows that implantation of an intrastromal corneal ring of this dimension (0.3-mm thick) can reduce approximately 1.50 to 3.00 D of myopia and maintain spectacle-corrected visual acuity.

Limbal and choroidal Cryptococcus infection in the acquired immunodeficiency syndrome.

PURPOSE: A 30-year-old patient with the acquired immunodeficiency syndrome (AIDS) had limbal nodules and multifocal choroidal lesions. METHODS: A biopsy of the limbal nodules was performed. RESULTS: The biopsy showed Cryptococcus neoformans surrounded by thick mucinous capsules without inflammatory cell infiltration. CONCLUSION: In the differential diagnosis of limbal mass in patients with AIDS, cryptococcal infection should be considered.

Infecçäo por Chlamydia trachomatis e fatores de risco em gestantes / Chlamydia trachomatis infection and risk factors in pregnant women

Introduçäo. Realizado estudo com a finalidade de avaliar a freqüência da infecçäo por C. trachomatis em gestantes de Säo Paulo. MATERIAL E MÉTODOS. Um grupo de 122 gestantes (80 adultas e 42 adolescentes) foram submetidas a exame obstétrico completo e pesquisa de C. trachomatis na cérvice, pela técnica de ELISA (Chlamydiazyme). RESULTADOS. Foram positivos 11 mulheres (9 por cento), sendo oito (10 por cento) adultas e três (7 por cento) adolescentes. CONCLUSÄO. Nesta populaçäo estudade em Säo Paulo a idade das pacientes, bem como eatado civil, número de parceiros sexuais, existência de outra doença sexualmente transmissível, presença de cervicite ou a ocorrência de abortos näo foram fatores de risco para a presença de C. trachomatis

[Chlamydia trachomatis infection and risk factors in pregnant women]. / Infecção por Chlamydia trachomatis e fatores de risco em gestantes.

INTRODUCTION: In order to assess the frequency of Chlamydia trachomatis infection in pregnancy in São Paulo (Brazil), a group of 122 successive pregnant women (80 adults and 42 adolescents) attending the pre-natal care clinic of the Department of Obstetrics were studied. MATERIAL AND METHODS: The exam consisted of an epidemiological survey, a complete obstetrical clinical examination and the assessment of the presence of C. trachomatis in the vagina by ELISA (Chlamydiazyme). RESULTS: A total of 11 women (9%) were positive, being 8 (10%) of them adults and 3 (7%) adolescents. CONCLUSION: In São Paulo the age of the patient as well as the marital status, the number of sexual partners, the existence of other sexually transmitted diseases, the presence of cervicitis or the occurrence of abortions could not be considered as risk factors for the presence of the Chlamydia trachomatis.

Achados oftalmológicos em AIDS: avaliaçäo de 445 casos atendidos em um ano / Ophtalmological manifestations in AIDS: evaluation of 445 patients in one year

Os autores avaliaram 445 pacientes HIV positivos e identificaram as manifestaçöes oftalmológicas presentes nesses pacientes. OBJETIVO. Identificar e correlacionar as alteraçöes oftalmológicas com dados de literatura, bem como correlacioná-las com as diversas manifestaçöes clínicas presentes na síndrome. MÉTODOS. Foram estudados 445 pacientes ambulatoriais HIV (com 66 por cento e sem 34 por cento AIDS), sendo 87 por cento do sexo masculino e 58,2 por cento homossexuais, no período de um ano, atendidos na Escola Paulista de Medicina, Hospital Säo Paulo. RESULTADOS. Do total de pacientes examinados, 52 por cento apresentaram alteraçöes oculares secundárias à infecçäo pelo HIV ao primeiro exame (27 por cento bilaterais). A retinite por citomegalovírus esteve presente em 25 por cento deles, seguida por toxoplasmose ocular (8,5 por cento), retinite por herpes (3,6 por cento), papiledema (2,2 por cento), atrofia óptica (1,6 por cento), phthisis bulbi (1,5 por cento), coroidite multifocal (1,2 por cento), hemorragia retiniana (0,9 por cento), uveíte por sífilis (0,6 por cento) e oclusäo da veia central da retina (0,2 por cento). CONCLUSAO. Nossos dados estäo de acordo com a literatura mundial, com exceçäo feita à retinocoroidite por toxoplasmose, que mostrou muito maior freqüência em nosso meio, se comparada à da literatura. Pudemos observar, também, uma alta taxa de descolamento de retina secundário à inflamaçäo intra-ocular. Nenhum caso de coroidite por Pneumocystis carinii foi diagnosticado nessa época

[Ophthalmological manifestations in AIDS: evaluation of 445 patients in one year]. / Achados oftalmológicos em AIDS: avaliação de 445 casos atendidos em um ano.

The authors prospectively evaluated 445 HIV positive patients for the presence of ophthalmological manifestations. PURPOSE--To evaluate patients HIV positive with or without AIDS and correlate the data with the ocular findings mentioned in the literature. METHODS--445 HIV positive patients (66% with AIDS) were evaluated in one year at the Paulista School of Medicine, São Paulo Hospital, Brazil. There was a predominance of males (87%) and homosexuals (58.2%). RESULTS--Of the 445 patients, 52% presented ocular findings secondary to HIV infection at the first examination. The diagnosis included: CMV retinitis (25%), ocular toxoplasmosis (8.5%), herpes retinitis (3.6%), papilledema (2.2%), optic atrophy (1.6%), phthisis bulbi (1.5%), multifocal choroiditis (1.2%), retinal hemorrhages (0.9%), syphilitic uveitis (0.6%) and central vein occlusion (0.2%). CONCLUSION--The incidence of ophthalmic manifestations of AIDS in Brazil is similar to that found in the international literature. We found though a higher incidence of ocular toxoplasmosis than that in other countries. No ocular pneumocystosis was presents in the population evaluated by us.

Intrastromal corneal ring--one-year results of first implants in humans: a preliminary nonfunctional eye study.

BACKGROUND: The Intrastromal Corneal Ring (ICR) is a new investigational medical device designed to alter corneal curvature without surgical intervention in the central cornea. The ring is inserted between the layers of the stroma in the cornea's periphery at two-thirds depth through an approximately 2-millimeter incision. METHODS: To investigate the safety and refractive effect of implanting an ICR of a given thickness (0.30 mm) and outer diameter (7.70 mm) into human corneas, an ICR was implanted into one nonfunctional eye of each of three patients during the period of March to May, 1991. One predesignated ICR was successfully explanted 5 months after implantation to evaluate the feasibility of ICR removal and to observe the effect of ring removal on corneal curvature. Patients were followed for 1 year after the initial implant procedure. RESULTS: The three implant procedures and postoperative courses proceeded without any significant complications. Approximately 2.00 D of central corneal flattening was achieved in all eyes. No adverse reactions or other medically-significant complications were observed over a 1-year follow-up period. The patient who underwent ICR removal experienced no perioperative complications, and the patient's cornea has remained stable with a return to its preoperative curvature. CONCLUSION: Although this study is preliminary and limited in scope, we have demonstrated that the ICR can be tolerated safely in the human cornea and results in a flattening of the corneal curvature that is stable for up to a year after insertion. The successful removal of the ICR begins to establish reversibility of the procedure and induced refractive effect.

An unusually high prevalence of ocular toxoplasmosis in southern Brazil.

Because of the frequency of ocular toxoplasmosis and its occurrence in multiple siblings in southern Brazil, a population-based household survey was performed to better understand the epidemiologic characteristics of the disease in this region. Of 1,042 individuals examined, 184 (17.7%) were deemed to have ocular toxoplasmosis on the basis of conservative assessment of ophthalmic findings. Of those with ocular toxoplasmosis, 183 (99.5%) had specific IgG antibodies, compared with only 140 of 181 age-matched control subjects (77.4%; P less than .001). The prevalence of ocular toxoplasmosis was 0.9% in 1- to 8-year-olds, 4.3% in 9- to 12-year-olds, 14.3% in 13- to 16-year-olds, and 21.3% (95% confidence interval, 18.6% to 24.2%) in all individuals 13 years or older. The prevalence of ocular toxoplasmosis in this population was more than 30 times higher than previous estimates for the same condition elsewhere. The low prevalence in the young children we studied supplements previous data suggesting that, in this population, ocular toxoplasmosis is a sequela of postnatal rather than congenital infection.

Intraocular lens implantation in anterior megalophthalmus. Case report.

We report three cases of extracapsular cataract extraction with intraocular lens implantation in two patients with anterior megalophthalmus. This is the first report, to our knowledge, of intraocular lens implantation in this rare condition.

Behçet's disease in Brazil--a review of 49 cases with emphasis on ophthalmic manifestations.

At the Uveitis Clinic of the Department of Ophthalmology of the Escola Paulista de Medicina, São Paulo, Brazil, Behçet's disease was diagnosed in 49 patients, representing 2.0% of the total uveitis cases attended during the 16-year period from February 1974 to June 1990. Of these, 71% were men. The age of onset of the disease was between 9 and 61 years, with a mean of 29.6 years. The ethnic distribution was the following: 76% Caucasian, 14% darkly pigmented, 8% Mongoloid and 2% Negroid. HLA-B5 was found in 45.5% of the 11 Caucasian patients typed. In 34.5% of the cases, the ocular attack was the initial manifestation, alone or associated with other symptoms. Oral aphthae were recorded in 98% of the patients, genital ulcers in 55.1%, and skin lesions in 51%. Joint involvement was present in 44.9%, neurologic symptoms were evidenced in 3 patients, and 2 patients had major vascular involvement. The mean interval between the first manifestation of the disease and the onset of ocular involvement was 3.1 years, with a range of 4 months to 14 years. The interval between affections of both eyes ranged from 0 to 2 years; in 38.4% of the cases it occurred within one month. Anterior and posterior segment involvement was seen in 85.7% of the patients. Hypopyon was observed in 34.7% of the cases. Seven patients did not present iridocyclitis at any time in the course of their disease. We did not see any cases with only anterior segment involvement.(ABSTRACT TRUNCATED AT 250 WORDS)

Estudo da flora fúngica da comjuntiva normal, da cana-de-açúcar e de anemófilos da regiäo canavieira de Santa Rita, Paraíba, Brasil / Study of the fungi flora in conjunctiva of sugar-cane workers and in the air and leaves of the sugar-cane plants in the Northest of Brazil

O estudo da microbiotica fúngica da conjuntiva sadia de 104 trabalhadores no corte da cana-de-açucar, no município de Santa Rita, Estado da Paraíba, apresentou como conclusöes que: 1) As culturas foram positivas em 38,5% dos indivíduos. 2) O fungo filamentoso mais comumente encontrado foi o Fusarium ssp (19,65%) sendo a espécie F. solani a mais freqüente (40,0%). Dentre as leveduras, destacou-se a Candida spp (50,0%) e a espécie C. albicans (43,58%). 3) Houve predominância dos fungos leveduriformes sobre os filamentosos. 4) A média do número de colônias por placas näo foi alta, mostrando uma microbiótica reduzida em cada trabalhador. 5) Fungos reconhecidamente patogênicos para o olho fizeram parte da microbiota fúngica. Nove gêneros diferentes foram identificados, mostrando uma microbióta variada. 6) A freqüência fúngica, quando comparada aos resultados de SCARPI, mostrou grande variaçäo. 7) A maioria dos fungos encontrados na conjuntiva foram isolados também na superfície epidérmica da folha de cana-de-açucar e colmo como também no ar, mostrando a participaçäo da contaminaçäo ambiental para conjuntiva. 8) Foi significante o aumento progressivo da freqüência fúngica em conjuntiva normal com o evoluir da idade. O estudo da microbiótica fúngica da cana-de açucar, epiderme colmo e da folha demonstrou que: 1) Os fungos isolados pertenciam a 13 gêneros. 2) O fungo mais frequentemente identificado foi o Fusarium spp., sendo o F. moniliforme na epiderme do colmo da cana, e o solani na epiderme da folha de cana os mais freqüentes. 3) Há maior variaçäo no gênereo entre os fungos presentes na folha. 4) A Candida albicans foi a levedura mais encontrada. 5) Os fungos mais freqüentemente encontrados por SCARPI, foram verificados em baixa freqüência nesse estudo e vice-versa. A análise da microbióta fúngica anemófila do canavial revelou que: 1) Identificaram-se 18 variedades de gêneros; 2) O fungo mais comumente isolado foi o Fusarium, e a espécie foi F. oxysporum; 3) A freqüência média de crescimento de fungo por placa foi alta; em 20 placas expostas ao meio ambiente, 18 mostraram crescimento fúngico; 4) Candida albicans foi a levedura identificada com maior freqüência. 5) Houve grande variedade fúngica nesse estudo, em comparaçäo com o trabalho de SCARPI