ABSTRACT
A study was made of the effect of leukocytic interferon on the phagocytic activity of alveolar macrophages and neutrophils in patients with chronic bronchitis, tuberculosis and sarcoidosis of the lungs. Cells isolated from the patients' bronchoalveolar washing off were used. It was concluded that short-term incubation of macrophages and neutrophils with interferon resulted in phagocytosis enhancement and increased intensity of metabolic processes in them. Interferon produced the most noticeable effect on cells with an initially reduced absorption capacity.
Subject(s)
Bronchi/pathology , Bronchitis/immunology , Interferon Type I/pharmacology , Lung Diseases/immunology , Macrophages/immunology , Neutrophils/immunology , Pulmonary Alveoli/pathology , Sarcoidosis/immunology , Adjuvants, Immunologic , Chronic Disease , Culture Media , Humans , In Vitro Techniques , Phagocytosis/drug effects , Time Factors , Tuberculosis, Pulmonary/immunologyABSTRACT
As the result of the study of the peripheral blood monocyte function in patients with pulmonary tuberculosis, the ingestive capacity of monocytes has been found to be suppressed, which indicates the pathological state of oxygen-dependent mechanisms governing the bactericidal activity of cells, the most pronounced disturbances of monocyte functions being observed in patients with fibrous-cavernous and disseminated tuberculosis.
Subject(s)
Monocytes/immunology , Tuberculosis, Pulmonary/immunology , Adult , Aged , Blood Bactericidal Activity , Female , Humans , Male , Middle Aged , Nitroblue Tetrazolium , PhagocytosisSubject(s)
Monocytes/immunology , Tuberculosis, Pulmonary/immunology , Adult , Aged , Female , Humans , Latex , Male , Microspheres , Middle Aged , Nitroblue Tetrazolium/metabolism , PhagocytosisABSTRACT
The aim of this investigation was to increase the accuracy of the determination of the functional activity of alveolar macrophages with phagocytic functions, isolated from bronchial washings, to reduce the time necessary for this determination and to compare the results thus obtained with the clinical signs of the disease. 46 patients with nonspecific pulmonary diseases were examined. The method of the simultaneous evaluation of the phagocytic activity of alveolar macrophages and the level of acidic phosphatase activity during phagocytosis was found to ensure greater accuracy in the determination of the functional activity of alveolar macrophages and to reduce the time necessary for the evaluation of the reaction by 1.5 hours. In patients with non-specific pulmonary diseases the phagocytic activity of alveolar macrophages decreased, which was accompanied by a lower level of acidic phosphatase consumption.