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1.
Life (Basel) ; 12(3)2022 Mar 16.
Article in English | MEDLINE | ID: mdl-35330182

ABSTRACT

The genome hypothesis postulates that genes in a genome tend to conform to their species' usage of the codon catalog and the GC content of the DNA. Thus, codon frequencies differ across organisms, including the three termination codons in the standard genetic code. Here, we analyze the frequencies of stop codons in a group of highly expressed genes from 196 prokaryotes under strong translational selection. The occurrence of the three translation termination codons is highly biased, with UAA (ochre) being the most prevalent in almost all bacteria. In contrast, UAG (amber) is the least frequent termination codon, e.g., only 321 occurrences (7.4%) in E. coli K-12 substr. W3110. Of the 253 highly expressed genes, only two end with an UAG codon. The strength of the selective bias against UAG in highly expressed genes varies among bacterial genomes, but it is not affected by the GC content of these genomes. In contrast, increased GC content results in a decrease in UAA abundance with a concomitant increase in UGA abundance. We propose that readthrough efficiency and context effects could explain the prevalence of UAA over UAG, particularly in highly expressed genes. Findings from this communication can be utilized for the optimization of gene expression.

2.
PLoS One ; 10(4): e0124309, 2015.
Article in English | MEDLINE | ID: mdl-25875362

ABSTRACT

Topoisomerases are enzymes that alter the topological properties of DNA. Phage T4 encodes its own topoisomerase but it can also utilize host-encoded topoisomerases. Here we characterized 55.2, a phage T4 predicted ORF of unknown function. High levels of expression of the cloned 55.2 gene are toxic in E. coli. This toxicity is suppressed either by increased topoisomerase I expression or by partial inactivation of the ATPase subunit of the DNA gyrase. Interestingly, very low-level expression of 55.2, which is non-lethal to wild type E. coli, prevents the growth of a deletion mutant of the topoisomerase I (topA) gene. In vitro, gp55.2 binds DNA and blocks specifically the relaxation of negatively supercoiled DNA by topoisomerase I. In vivo, expression of gp55.2 at low non-toxic levels alters the steady state DNA supercoiling of a reporter plasmid. Although 55.2 is not an essential gene, competition experiments indicate that it is required for optimal phage growth. We propose that the role of gp55.2 is to subtly modulate host topoisomerase I activity during infection to insure optimal T4 phage yield.


Subject(s)
Bacteriophage T4/genetics , DNA Topoisomerases, Type I/metabolism , Escherichia coli/enzymology , Open Reading Frames/genetics , Bacteriophage T4/growth & development , Bacteriophage T4/physiology , Cloning, Molecular , DNA Topoisomerases, Type I/chemistry , DNA Topoisomerases, Type I/genetics , DNA, Superhelical/chemistry , DNA, Superhelical/metabolism , Electrophoretic Mobility Shift Assay , Escherichia coli/growth & development , Escherichia coli/virology , Plasmids/genetics , Plasmids/metabolism , Protein Binding , Recombinant Fusion Proteins/biosynthesis , Recombinant Fusion Proteins/genetics , Topoisomerase I Inhibitors/chemistry , Topoisomerase I Inhibitors/metabolism , Viral Proteins/chemistry , Viral Proteins/genetics , Viral Proteins/metabolism
3.
J Bacteriol ; 197(3): 542-52, 2015 Feb.
Article in English | MEDLINE | ID: mdl-25404704

ABSTRACT

Protein export to the bacterial periplasm is achieved by SecYEG, an inner membrane heterotrimer. SecY and SecE are encoded by essential genes, while SecG is not essential for growth under standard laboratory conditions. Using a quantitative and sensitive export assay, we show that SecG plays a critical role for the residual export mediated by mutant signal sequences; the magnitude of this effect is not proportional to the strength of the export defect. In contrast, export mediated by wild-type signal sequences is only barely retarded in the absence of SecG. When probed with mutant signal sequences, secG loss of function mutations display a phenotype opposite to that of prlA mutations in secY. The analysis of secG and prlA single and double mutant strains shows that the increased export conferred by several prlA alleles is enhanced in the absence of SecG. Several combinations of prlA alleles with a secG deletion cannot be easily constructed. This synthetic phenotype is conditional, indicating that cells can adapt to the presence of both alleles. The biochemical basis of this phenomenon is linked to the stability of the SecYE dimer in solubilized membranes. With prlA alleles that can be normally introduced in a secG deletion strain, SecG has only a limited effect on the stability of the SecYE dimer. With the other prlA alleles, the SecYE dimer can often be detected only in the presence of SecG. A possible role for the maintenance of SecG during evolution is proposed.


Subject(s)
Escherichia coli Proteins/metabolism , Escherichia coli/metabolism , Membrane Proteins/metabolism , Protein Sorting Signals , Protein Transport , Escherichia coli/genetics , Escherichia coli Proteins/genetics , Gene Deletion , Membrane Proteins/genetics , Protein Multimerization , Protein Stability , SEC Translocation Channels
4.
J Appl Toxicol ; 32(3): 233-43, 2012 Mar.
Article in English | MEDLINE | ID: mdl-22223356

ABSTRACT

Aluminium salts used as antiperspirants have been incriminated as contributing to breast cancer incidence in Western societies. To date, very little or no epidemiological or experimental data confirm or infirm this hypothesis. We report here that in MCF-10A human mammary epithelial cells, a well-established normal human mammary epithelial cell model, long-term exposure to aluminium chloride (AlCl(3) ) concentrations of 10-300 µ m, i.e. up to 100 000-fold lower than those found in antiperspirants, and in the range of those recently measured in the human breast, results in loss of contact inhibition and anchorage-independent growth. These effects were preceded by an increase of DNA synthesis, DNA double strand breaks (DSBs), and senescence in proliferating cultures. AlCl(3) also induced DSBs and senescence in proliferating primary human mammary epithelial cells. In contrast, it had no similar effects on human keratinocytes or fibroblasts, and was not detectably mutagenic in bacteria. MCF-10A cells morphologically transformed by long-term exposure to AlCl(3) display strong upregulation of the p53/p21(Waf1) pathway, a key mediator of growth arrest and senescence. These results suggest that aluminium is not generically mutagenic, but similar to an activated oncogene, it induces proliferation stress, DSBs and senescence in normal mammary epithelial cells; and that long-term exposure to AlCl(3) generates and selects for cells able to bypass p53/p21(Waf1) -mediated cellular senescence. Our observations do not formally identify aluminium as a breast carcinogen, but challenge the safety ascribed to its widespread use in underarm cosmetics.


Subject(s)
Aluminum Compounds/toxicity , Antiperspirants/toxicity , Breast/drug effects , Chlorides/toxicity , Aluminum Chloride , Breast/pathology , Cell Line, Tumor , Cell Proliferation/drug effects , Cell Transformation, Neoplastic , DNA Breaks, Double-Stranded , DNA Repair , Epithelial Cells/drug effects , Female , Humans
5.
Mol Microbiol ; 82(6): 1406-21, 2011 Dec.
Article in English | MEDLINE | ID: mdl-22029793

ABSTRACT

Phage T4, the archetype of lytic bacterial viruses, needs only 62 genes to propagate under standard laboratory conditions. Interestingly, the T4 genome contains more than 100 putative genes of unknown function, with few detectable homologues in cellular genomes. To characterize this uncharted territory of genetic information, we have identified several T4 genes that prevent bacterial growth when expressed from plasmids under inducible conditions. Here, we report on the various phenotypes and molecular characterization of 55.1, one of the genes of unknown function. High-level expression from the arabinose-inducible P(BAD) promoter is toxic to the bacteria and delays the intracellular accumulation of phage without affecting the final burst size. Low-level expression from T4 promoter(s) renders bacteria highly sensitive to UV irradiation and hypersensitive to trimethoprim, an inhibitor of dihydrofolate reductase. The delay in intracellular phage accumulation requires UvsW, a T4 helicase that is also a suppressor of 55.1-induced toxicity and UV sensitivity. Genetic and biochemical experiments demonstrate that gp55.1 binds to FolD, a key enzyme of the folate metabolism and suppressor of 55.1. Finally, we show that gp55.1 prevents the repair of UV-induced DNA photoproducts by the nucleotide excision repair (NER) pathway through interaction with the UvrA and UvrB proteins.


Subject(s)
Bacteriophage T4/genetics , DNA Repair , Escherichia coli/genetics , Escherichia coli/metabolism , Folic Acid/metabolism , Gene Expression , Viral Proteins/metabolism , Bacteriophage T4/metabolism , DNA Damage/radiation effects , DNA Repair/radiation effects , Escherichia coli/radiation effects , Escherichia coli Proteins/genetics , Escherichia coli Proteins/metabolism , Ultraviolet Rays , Viral Proteins/genetics
6.
Res Microbiol ; 161(8): 706-10, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20674740

ABSTRACT

Microcin E492 (MccE492) is an antibacterial protein whose activity on target cells requires ManYZ, the inner membrane component of the mannose permease. We show here that MceA, the polypeptide core of MccE492, stably associates with ManYZ both in the presence and in the absence of MceB, the MccE492 immunity protein. The two known physiological activities of the mannose permease were assayed in cells co-expressing MceA and MceB. Under these conditions, growth on mannose as the sole carbon source is prevented; this was not observed in cells expressing only MceB. In contrast, susceptibility to bacteriophage λ infection was not affected.


Subject(s)
Anti-Bacterial Agents/metabolism , Bacteriocins/chemistry , Bacteriocins/metabolism , Klebsiella pneumoniae/metabolism , Mannose/metabolism , Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism , Anti-Bacterial Agents/chemistry , Antibiosis , Bacterial Proteins/metabolism , Bacteriophage lambda/physiology , Cell Membrane/metabolism , Klebsiella pneumoniae/enzymology , Membrane Proteins/metabolism , Microbial Viability , Phosphoenolpyruvate Sugar Phosphotransferase System/chemistry
7.
Methods Mol Biol ; 619: 103-16, 2010.
Article in English | MEDLINE | ID: mdl-20419406

ABSTRACT

General protein export requires the cooperation of two elements, the Sec translocase and a signal sequence. The interactions of both wild type and mutant components can be studied in vivo using a number of genetic systems. Signal sequence mutations that prevent export have been characterized ("down mutations"). Suppressors of these signal sequence mutations, known as prl mutations, have been isolated in most sec genes. More recently, inactive N-terminal regions of cytoplasmic proteins were converted into active signal sequences ("up mutations").Alkaline phosphatase (PhoA), an enzyme only active after export to the periplasm, provides the best and most versatile quantitative reporter for protein translocation studies. Cleavable signal sequences can be used to monitor protein export in a time frame of 15-120 s. Chimeric proteins expressed from an inducible promoter can be used to measure kinetics of enzyme accumulation in a time frame of 10-100 min. Finally, the export activity of PhoA-chimeras can be visualized in a semi-quantitative way by staining colonies growing on Petri dishes with a chromogenic substrate, in the time frame of 10-40 h.


Subject(s)
Escherichia coli Proteins/metabolism , Escherichia coli/metabolism , Periplasm/metabolism , Protein Transport/physiology , Alkaline Phosphatase/genetics , Alkaline Phosphatase/metabolism , Electrophoresis, Polyacrylamide Gel , Escherichia coli/genetics , Escherichia coli Proteins/genetics , Immunoprecipitation , Membrane Proteins/genetics , Membrane Proteins/metabolism , Protein Sorting Signals/genetics , Protein Sorting Signals/physiology , Protein Transport/genetics , Serine Endopeptidases/genetics , Serine Endopeptidases/metabolism
8.
Science ; 325(5941): 753-6, 2009 Aug 07.
Article in English | MEDLINE | ID: mdl-19661432

ABSTRACT

Protein secretion occurs via translocation by the evolutionarily conserved Sec complex. LacZ hybrid proteins have long been used to study translocation in Escherichia coli. Some LacZ hybrids were thought to block secretion by physically jamming the Sec complex, leading to cell death. We found that jammed Sec complexes caused the degradation of essential translocator components by the protease FtsH. Increasing the amounts or the stability of the membrane protein YccA, a known inhibitor of FtsH, counteracted this destruction. Antibiotics that inhibit translation elongation also jammed the translocator and caused the degradation of translocator components, which may contribute to their effectiveness. Intriguingly, YccA is a functional homolog of the proto-oncogene product Bax Inhibitor-1, which may share a similar mechanism of action in regulating apoptosis upon prolonged secretion stress.


Subject(s)
Anti-Bacterial Agents/pharmacology , Escherichia coli Proteins/metabolism , Escherichia coli/drug effects , Escherichia coli/metabolism , Membrane Proteins/metabolism , Protein Transport , ATP-Dependent Proteases/metabolism , Bacterial Outer Membrane Proteins/genetics , Bacterial Outer Membrane Proteins/metabolism , Chloramphenicol/pharmacology , Escherichia coli/genetics , Escherichia coli Proteins/genetics , Heat-Shock Proteins/genetics , Heat-Shock Proteins/metabolism , Membrane Proteins/genetics , Mutant Proteins/metabolism , Periplasmic Proteins/genetics , Periplasmic Proteins/metabolism , Porins/genetics , Porins/metabolism , Protein Sorting Signals , Protein Transport/drug effects , Receptors, Virus/genetics , Receptors, Virus/metabolism , Recombinant Fusion Proteins/metabolism , SEC Translocation Channels , Serine Endopeptidases/genetics , Serine Endopeptidases/metabolism , Tetracycline/pharmacology , beta-Galactosidase/genetics , beta-Galactosidase/metabolism
9.
Development ; 135(5): 859-67, 2008 Mar.
Article in English | MEDLINE | ID: mdl-18216177

ABSTRACT

Myoblast differentiation is essential to skeletal muscle formation and repair. The earliest detectable event leading to human myoblast differentiation is an upregulation of Kir2.1 channel activity, which causes a negative shift (hyperpolarization) of the resting potential of myoblasts. After exploring various mechanisms, we found that this upregulation of Kir2.1 was due to dephosphorylation of the channel itself. Application of genistein, a tyrosine kinase inhibitor, increased Kir2.1 activity and triggered the differentiation process, whereas application of bpV(Phen), a tyrosine phosphatase inhibitor, had the opposite effects. We could show that increased Kir2.1 activity requires dephosphorylation of tyrosine 242; replacing this tyrosine in Kir2.1 by a phenylalanine abolished inhibition by bpV(Phen). Finally, we found that the level of tyrosine phosphorylation in endogenous Kir2.1 channels is considerably reduced during differentiation when compared with proliferation. We propose that Kir2.1 channels are already present at the membrane of proliferating, undifferentiated human myoblasts but in a silent state, and that Kir2.1 tyrosine 242 dephosphorylation triggers differentiation.


Subject(s)
Cell Differentiation , Myoblasts/cytology , Myoblasts/physiology , Phosphotyrosine/metabolism , Potassium Channels, Inwardly Rectifying/genetics , Tyrosine , Amino Acid Sequence , Biotinylation , Cell Culture Techniques , Cell Division , Cell Membrane/physiology , DNA/genetics , Electrophysiology , Electroporation , Gene Expression Regulation , Humans , Potassium Channels, Inwardly Rectifying/chemistry , Potassium Channels, Inwardly Rectifying/metabolism , Transfection , Tyrosine/metabolism , Up-Regulation
10.
J Biol Chem ; 282(2): 1281-7, 2007 Jan 12.
Article in English | MEDLINE | ID: mdl-17092931

ABSTRACT

Previous studies have shown that the SecY plug is displaced from the center of the SecYEG channel during polypeptide translocation. The structural and functional consequences of the deletion of the plug are now examined. Both in vivo and in vitro observations indicate that the plug domain is not essential to the function of the translocon. In fact, deletion of the plug confers to the cell and to the membranes a Prl-like phenotype: reduced proton-motive force dependence of translocation, increased membrane insertion of SecA, diminished requirement for functional leader peptide, and weakened SecYEG subunit association. Although the plug domain does not seem essential, locking the plug in the center of the channel inactivates the translocon. Thus, the SecY plug is important to regulate the activity of the channel and to confer specificity to the translocation reaction. We propose that the plug contributes to the gating mechanism of the channel by maintaining the structure of the SecYEG complex in a compact closed state.


Subject(s)
Escherichia coli Proteins/chemistry , Escherichia coli Proteins/metabolism , Escherichia coli/metabolism , Multiprotein Complexes/chemistry , Multiprotein Complexes/metabolism , Crystallography , Membrane Proteins/chemistry , Membrane Proteins/metabolism , Protein Structure, Quaternary , Protein Structure, Secondary , Protein Structure, Tertiary , Protein Transport/physiology , SEC Translocation Channels
11.
J Bacteriol ; 188(20): 7049-61, 2006 Oct.
Article in English | MEDLINE | ID: mdl-17015644

ABSTRACT

Microcin E492 (MccE492) is a bactericidal protein secreted by Klebsiella pneumoniae that is active against various species of Enterobacteriaceae. Interaction of MccE492 with target cells leads to the depolarization and permeabilization of their inner membranes. Several MccE492-specific proteins are required for the maturation and secretion of active MccE492. Surprisingly, the expression of only MceA, the polypeptide backbone of MccE492, is shown here to be toxic by itself. We refer to this phenomenon as endogenous MceA bactericidal activity to differentiate it from the action of extracellularly secreted MccE492. The toxicity of endogenous MceA is enhanced by an efficient targeting to the inner membrane. However, a periplasmic intermediate state is not required for MceA toxicity. Indeed, endogenous MceA remains fully active when it is fused to thioredoxin-1, a fast-folding protein that promotes retention of the C terminus of MceA in the cytoplasm. The C-terminal domain of MccE492 is required only for delivery from the extracellular environment to the periplasm, and it is not required for inner membrane damage. A common component is absolutely essential for the bactericidal activity of both endogenous MceA and extracellular MccE492. Indeed, toxicity is strictly dependent on the presence of ManYZ, an inner membrane protein complex involved in mannose uptake. Based on these findings, we propose a new model for cell entry, inner membrane insertion, and toxic activity of MccE492.


Subject(s)
Bacteriocins/metabolism , Bacteriocins/toxicity , Escherichia coli/drug effects , Klebsiella pneumoniae/metabolism , Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism , Anti-Bacterial Agents/metabolism , Anti-Bacterial Agents/toxicity , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Bacterial Proteins/toxicity , Bacteriocins/chemistry , Bacteriocins/genetics , Cell Membrane/metabolism , Escherichia coli/genetics , Escherichia coli Proteins/genetics , Escherichia coli Proteins/physiology , Microbial Viability , Models, Biological , Phosphoenolpyruvate Sugar Phosphotransferase System/genetics , Protein Transport/genetics
12.
Hum Gene Ther ; 16(10): 1157-67, 2005 Oct.
Article in English | MEDLINE | ID: mdl-16218777

ABSTRACT

The urokinase plasminogen activator (uPA) is implicated in both cancer cell invasion and angiogenesis. It can interact with a specific receptor (uPAR) via the epidermal growth factor (EGF)-like domain in the urokinase amino-terminal fragment (ATF) in a species-specific manner. Our previous studies showed that adenovirusmediated delivery of murine ATF (AdmATF) suppressed human tumor growth in mouse models, by inhibiting murine angiogenesis. However, we cannot exclude its putative inhibitory action on human cancer cell invasion through a uPAR-independent pathway. To further investigate the mechanisms of ATF, we constructed another adenovirus, AdhmATF, expressing humanized murine ATF (hmATF). hmATF binds to human uPAR but not to murine uPAR. We compared the antagonist effect of both AdmATF and AdhmATF on human and murine cancer cells. In vitro, the supernatant from AdhmATF-infected cells repressed 79% of membrane-associated uPA activity on human MDA-MB-231 cells, whereas that from AdmATF-infected cells repressed 35% of membrane-associated uPA activity. On murine LLC cells, the supernatant from AdhmATF-infected cells inhibited 29% of cell surface uPA activity, whereas that from AdmATF-infected cells inhibited 74% of cell surface uPA activity. Similar results were obtained in a cell invasion assay. In vivo, intratumoral injection of the adenoviruses into LLC tumors on day 24 postinjection induced lower but significant tumor growth suppression by AdhmATF (tumor volume was 1185 +/- 128 mm3), whereas suppression by AdmATF was greater (407 +/- 147 mm3). In the MDA-MB-231 tumor model, on day 52 postinjection, tumor size was 187 +/- 47 mm3 in the AdhmATF-treated group and 468 +/- 65 mm3 in the AdmATF-treated group. The LLC and MDA-MB- 231 cell lines transfected by mATF or hmATF genes showed growth inhibition In vivo equivalent to the results obtained by adenovirus treatment. These results demonstrate the strong anticancer activity of ATF even when its uPAR-binding affinity has been suppressed, and indicate that ATF exerts an antitumor effect via dual mechanisms: essentially through targeting the uPA-uPAR system via the EGF-like domain and partially through targeting a uPAR-independent interaction via the kringle domain.


Subject(s)
Adenoviridae , Genetic Therapy , Genetic Vectors , Neoplasms, Experimental/therapy , Receptors, Cell Surface/metabolism , Urokinase-Type Plasminogen Activator , Animals , Cell Line, Tumor , Female , Genetic Therapy/methods , Humans , Mice , Mice, Mutant Strains , Neoplasm Invasiveness , Neoplasm Transplantation , Neoplasms, Experimental/metabolism , Neoplasms, Experimental/pathology , Peptide Fragments/administration & dosage , Peptide Fragments/genetics , Receptors, Urokinase Plasminogen Activator , Urokinase-Type Plasminogen Activator/administration & dosage , Urokinase-Type Plasminogen Activator/chemistry , Urokinase-Type Plasminogen Activator/genetics
14.
Genetics ; 162(3): 1031-43, 2002 Nov.
Article in English | MEDLINE | ID: mdl-12454053

ABSTRACT

The murine plasminogen activator inhibitor 2 (PAI2) signal sequence inefficiently promotes the export of E. coli alkaline phosphatase (AP). High-level expression of PAI2::AP chimeric proteins from the arabinose P(BAD) promoter is toxic and confers an Ara(S) phenotype. Most Ara(R) suppressors map to secA, as determined by sequencing 21 independent alleles. Mutations occur throughout the gene, including both nucleotide binding domains (NBDI and NBDII) and the putative signal sequence binding domain (SSBD). Using malE and phoA signal sequence mutants, we showed that the vast majority of these secA suppressors exhibit weak Sec phenotypes. Eight of these secA mutations were further characterized in detail. Phenotypically, these eight suppressors can be divided into three groups, each localized to one domain of SecA. Most mutations allow near-normal levels of wild-type preprotein export, but they enhance the secretion defect conferred by signal sequence mutations. Interestingly, one group exerts a selective effect on the export of PAI2::AP when compared to that of AP. In conclusion, this novel class of secA mutations, selected as suppressors of a toxic signal sequence, differs from the classical secA (prlD) mutations, selected as suppressors of defective signal sequences, although both types of mutations affect signal sequence recognition.


Subject(s)
Adenosine Triphosphatases/genetics , Bacterial Proteins , Escherichia coli Proteins/genetics , Escherichia coli/physiology , Membrane Transport Proteins/genetics , Protein Sorting Signals/genetics , Protein Transport/physiology , Adenosine Triphosphatases/metabolism , Alkaline Phosphatase/genetics , Alkaline Phosphatase/metabolism , Animals , DNA Mutational Analysis , Escherichia coli/genetics , Escherichia coli Proteins/metabolism , Membrane Transport Proteins/metabolism , Mice , Mutation , Periplasmic Binding Proteins/genetics , Periplasmic Binding Proteins/metabolism , Plasminogen Activator Inhibitor 2/genetics , Plasminogen Activator Inhibitor 2/metabolism , Protein Sorting Signals/physiology , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , SEC Translocation Channels , SecA Proteins , Selection, Genetic
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