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1.
Mem Inst Oswaldo Cruz ; 112(6): 456-457, 2017 Jun.
Article in English | MEDLINE | ID: mdl-28591407

ABSTRACT

Triatoma infestans is an insect of subfamily Triatominae (Hemiptera: Reduviidae) and an important vector of Trypanosoma cruzi, the etiologic agent of human Chagas disease. In this work we reported a transcriptome assembly and annotation of T. infestans heads obtained by Next Generation Sequencing (NGS) technologies.


Subject(s)
Gene Expression Profiling , High-Throughput Nucleotide Sequencing , Insect Vectors/genetics , Triatoma/genetics , Animals
2.
Acta Trop ; 99(2-3): 144-54, 2006 Oct.
Article in English | MEDLINE | ID: mdl-17055993

ABSTRACT

In epidemiological terms, Panstrongylus megistus is one of the most important species of triatomine bug in Brazil. Samples from 11 localities were studied using the random amplification of polymorphic DNA (RAPD) technique, which was able to differentiate the study populations clearly. Biogeographical data indicate that these populations could already have arisen 18,000 years ago (C(14)), it being possible to differentiate insects from the Brazilian states of Santa Catarina (SC) in the south, Ceará (CE) in the northeast and another large intermediate block containing the remaining eight populations from five other states. These results agree with those obtained by phenograms constructed from RAPD data, in which the SC population lies opposite those of CE, consistent with the greatest geographical distance between these localities. The other eight populations (Alagoas (AL), Bahia (BA), Goiás (GO), Minas Gerais (MG) and São Paulo (SP)) are closer genetically and originated in areas whose vegetational characteristics have remained similar to each other during the last 18,000 years, thus allowing greater contact between them. The greatest divergence of this group of insects and those of Ceará appears to have occurred 8000 years ago. This more humid period gave rise to other landscape changes, allowing greater differentiation of the vegetation and consequent expansion of P. megistus populations. Formation of the Serras do Mar and Mantiqueira probably created geographical barriers that favored a certain degree of isolation and greater differentiation of the SC population. Atlantic forest remnants within the caatinga domain (created between 25 and 17,000 years ago), where the CE populations originated probably constitute ecological refugia produced by successive amplification and retraction of the most suitable habitats for this species.


Subject(s)
Ecosystem , Insect Vectors/genetics , Panstrongylus/genetics , Animals , Brazil , DNA/chemistry , DNA/genetics , Female , Genetic Variation , Male , Paleontology , Plants , Random Amplified Polymorphic DNA Technique
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