ABSTRACT
There was investigated the expression of molecular and cellular regulators of apoptosis Bcl-2 and Bad in the regional lymph nodes of the liver in rats exposed to the magnetic field of 50 Hz during 14 days. There was revealed a reduction in the protein expression of regulators of apoptosis Bcl-2 in the cortical area of the regional lymph nodes of the liver in animals exposed to the magnetic field.
Subject(s)
Apoptosis/physiology , Environmental Illness/metabolism , Genes, bcl-2/physiology , Liver/metabolism , Lymph Nodes/metabolism , bcl-Associated Death Protein/metabolism , Animals , Disease Models, Animal , Environmental Illness/pathology , Liver/pathology , Liver/radiation effects , Lymph Nodes/pathology , Lymph Nodes/radiation effects , Magnetic Fields , Male , Rats , Rats, WistarABSTRACT
There was investigated the response of cytoplasmic organelles of rat liver hepatocytes to the exposure of impact of power frequency magnetic field (magnetic field intensity: 5 - 10 microT, exposure time: 14 days). A statistically significant reduction in the number of hepatocytes and volume of mitochondria, the decline of the number of primary lysosomes and peroxisomes, the increase of granular and agranular reticulum has been established.
Subject(s)
Hepatocytes/metabolism , Magnetic Fields , Mitochondria, Liver/metabolism , Organelles/metabolism , Animals , Lysosomes/metabolism , Male , Peroxisomes/metabolism , Rats , Rats, WistarABSTRACT
We studied the effects of benzo(a)pyrene on lymphocyte chromatin in mice with experimental desynchronosis. Desynchronosis altered chromatin structure in immune system cells and suppressed the immune response. In mice with experimental desynchronosis, benzo(a)pyrene produced more pronounced changes in the chromatin structure compared to the control. It was inferred that light-dark cycle disturbances impair the resistance of organs and systems to damaging environmental factors.
Subject(s)
Benzo(a)pyrene/toxicity , Chromatin/drug effects , Circadian Rhythm/immunology , Lymphocytes/chemistry , Photoperiod , Animals , Chromatin/ultrastructure , Image Processing, Computer-Assisted , Male , Mice , Microscopy , Statistics, NonparametricABSTRACT
The effects of combined action of the industrial frequency magnetic field (MF) and continuous illumination (CI) on microcirculation, ultrastructural features of liver cells and apoptosis were studied in Wistar rats (n=56). It was found that in the liver of rats exposed to MF, CI and CI+MF for 14 days, parenchyma relative area was reduced, and that occupied by the sinusoidal capillaries was increased together with the liver vascularization coefficient. Combined exposure resulted in most pronounced ultrastructural changes (plasma membrane lysis in hepatocytes and endothelial cells, doubling of osmiophilic layers in tight intercellular junctions). Hepatocytes revealed condensed cytoplasm, mitochondria with electron-dense matrix, nuclear chromatin disintegration and nucleolar compaction. Hepatocyte intranuclear endonucleases were activated.
Subject(s)
Cell Nucleus , Endonucleases/metabolism , Hepatocytes , Lighting/adverse effects , Liver , Magnetics , Animals , Apoptosis , Cell Membrane/metabolism , Cell Membrane/ultrastructure , Cell Nucleus/enzymology , Cell Nucleus/ultrastructure , Cytoplasm/metabolism , Cytoplasm/ultrastructure , Hepatocytes/enzymology , Hepatocytes/ultrastructure , Liver/enzymology , Liver/ultrastructure , Male , Mitochondria, Liver/metabolism , Mitochondria, Liver/ultrastructure , Rats , Rats, Wistar , Time FactorsABSTRACT
We studied the effect of isolated and combined exposure to 24-h illumination and industrial frequency magnetic field on the expression of intracellular molecular apoptosis regulator Bcl-2 in the liver. Previous experiments showed enhanced expression of Bcl-2 protein in liver cells in animals maintained under conditions of 24-h illumination, whereas exposure to industrial frequency magnetic field had practically no effect on this process. Under conditions of combined exposure to 24-h illumination and industrial frequency magnetic field, magnetic field partially suppressed the expression of Bcl-2 induced by 24-h illumination.
Subject(s)
Apoptosis/radiation effects , Lighting/methods , Liver/radiation effects , Magnetics/methods , Proto-Oncogene Proteins c-bcl-2/metabolism , Animals , Circadian Rhythm , Environmental Exposure/adverse effects , Industry , Liver/metabolism , Liver/pathology , Liver/ultrastructure , Rats , Rats, Wistar , Time FactorsSubject(s)
Blood Cells/radiation effects , Electromagnetic Fields/adverse effects , Occupational Exposure , Animals , Blood Cells/immunology , Cells, Cultured , Data Interpretation, Statistical , Glucosephosphate Dehydrogenase/blood , L-Lactate Dehydrogenase/blood , Lymphocytes/enzymology , Lymphocytes/radiation effects , Male , Phagocytosis , Rats , Rats, Wistar , Succinate Dehydrogenase/blood , Time FactorsABSTRACT
The aim of this research was to study the morpho-functional organization of liver and some organs of immune system in animals exposed to continous twenty-four-hour illumination (CI) for 14 days. Using the methods of light and electron microscopy and morphometric analysis of histological preparations, liver and regional lymph nodes (hepatic in rats and iliac in mice) were studied. It was found that CI resulted in a state of dyschronosis, which was structurally manifested by the changes of diurnal variations of morphometric parameters of liver and its draining lymph nodes, disturbances of hemo- and lymphocirculation, destruction of cellular membranes. Response of central and peripheral organs of immune system to CI included the changes of diurnal variations of blast forms and mature lymphocytes, that was also indicative of dyschronosis development.
Subject(s)
Chronobiology Disorders/pathology , Light , Liver/ultrastructure , Lymph Nodes/ultrastructure , Animals , Chronobiology Disorders/immunology , Immune System/physiology , Immune System/ultrastructure , Liver/physiopathology , Lymph Nodes/physiopathology , Male , Mice , RatsABSTRACT
The aim of this research was to study the morpho-functional organization of liver and its regional lymph nodes, as well as of other organs of immune system, in animals exposed for 14 days to a magnetic field of industrial frequency (MFIF) with aperiodic oscillations of amplitude of magnetic induction in the range of 5-10 _Ts. The methods of light and electron microscopy were used. Damage to DNA in hepatocyte nuclei was determined using ethidium bromide. Quantitative analysis of the cells in the organs of immune system was performed, blood leukocyte formula was calculated and the activity of oxidative enzymes in blood cells was determined. It was found that MFIF caused micro- and ultrastructural changes in animal liver and its regional lymph nodes, which included the disturbances of hemo- and lymphocirculation, destruction of cellular membranes in some cells, dilation of rough endoplasmic reticulum cisterns, appearance of degenerate mitochondrial forms, glycogen depletion, that collectively indicated the development of tissue hypoxia. The increase in the activity of nuclear endonucleases was detected. Response of organs of immune system to MFIF was manifested by shifts of blast forms/ mature lymphocytes ratio in hepatic regional lymph nodes and by the changes of energy metabolism of blood lymphocytes.
Subject(s)
Liver/ultrastructure , Lymph Nodes/ultrastructure , Magnetics , Animals , Cell Nucleus/chemistry , DNA/analysis , DNA Damage , Hepatocytes/ultrastructure , L-Lactate Dehydrogenase/analysis , Liver/blood supply , Lymphocytes/enzymology , Male , Rats , Rats, Wistar , Regional Blood Flow , Spleen/blood supply , Spleen/ultrastructure , Succinate Dehydrogenase/analysis , Thymus Gland/blood supply , Thymus Gland/ultrastructureABSTRACT
The paper presents data on the impact of vibration (32 Hz) on spontaneous mutagenesis in mammals. Higher counts of micronuclei were found in the bone marrow polychromophilic red blood cells in rats on day 5 of exposure to vibration, there was a reduction in their counts by day 20 and their recovery by day 30, and their steady-state decrease within 30 days after vibration discontinuation. The involvement of emotional stress in the mechanism of antimutagenic action of vibration is considered.
