ABSTRACT
The disposition of topical dimethylacetylenedicarboxylate (DMAD) in tissue and its effect on glucose metabolism were studied in vivo, using skin grafted athymic nude mice, and in vitro, using excised pig skin. [14C]DMAD that penetrated skin grafts was distributed throughout the body. At 24 hr, the liver contained 15.62% of the applied dose. The kidneys, lungs, brain, and the heart contained 12.73, 5.61, 0.36, and 3.24% of the dose, respectively. One hour postapplication, DMAD markedly decreased [U-14C]glucose oxidation and the syntheses of fatty acids and glycogen in the livers and skin grafts. Similar effects were observed in excised pig skin. In addition, the activities of hepatic glucose-6-phosphate dehydrogenase, isocitric and NADP-malic dehydrogenase, and acetyl-CoA carboxylase were significantly reduced in DMAD-treated mice. In contrast, no effect was observed on the activity of glucokinase. The data indicate that DMAD rapidly penetrates the skin and causes aberrations in the activities of the glycogenic, lipogenic, and tricarboxylic acid metabolic pathways.
Subject(s)
Acetylene/analogs & derivatives , Irritants/toxicity , Acetylene/administration & dosage , Acetylene/pharmacology , Acetylene/toxicity , Administration, Topical , Alkynes , Animals , Fatty Acids/metabolism , Glucose/metabolism , In Vitro Techniques , Liver Glycogen/metabolism , Male , Mice , Mice, Nude , Oxidation-Reduction , Skin Absorption , Swine , Tissue DistributionSubject(s)
Aging/psychology , Cross-Cultural Comparison , Family , Social Environment , Aged , Female , Humans , Korea/ethnology , Male , United StatesABSTRACT
A prospective randomised trial (534 patients, 1969-75) was designed to determine whether radical mastectomy conferred advantages over modified radical mastectomy for breast cancer in terms of total survival, local recurrence, distant metastasis, and disease-free interval. The results showed no significant difference in outcome as regards these variables between the two treatments.
