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1.
Transplantation ; 70(7): 1074-80, 2000 Oct 15.
Article in English | MEDLINE | ID: mdl-11045645

ABSTRACT

BACKGROUND: Prevention of acute rejection is the most prevalent measure used to reduce the long-term risk of chronic allograft rejection. Until now, biopsy was the only useful diagnostic tool for monitoring allograft acute rejection, but invasiveness of this procedure limits its use. The aim of this study was to investigate the implication of peripheral blood immune markers as a predictive diagnostic tool preceding biopsy in acute renal allograft rejection determination. METHODS: Of the 61 patients studied, 13 had no rejection episodes, 8 had a proven acute rejection, and 40 were excluded for graft dysfunction causes. Mitogen-induced peripheral blood mononuclear cells were tested for interleukin- (IL) 2, IL-4, IL-5, IL-6, IL-10, IL-15, Interferon-gamma, Perforin, Granzyme B, and Fas L using semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). An up-regulated mRNA expression value was calculated in which a patient's sample was deemed positive if its differential expression value was equal or higher than the mean differential expression value calculated from the nonrejecting patients. RESULTS: IL-4, IL-5, IL-6, Interferon-gamma, Perforin, and Granzyme B mRNA levels were associated with acute rejection. When at least two of these cytokine markers were up-regulated in a given patient, 75% of the rejecting recipients were identified against 15% of the nonrejecting patients. CONCLUSIONS: We have shown that acute rejection episodes in renal transplant recipients were associated with an increase in mRNA expression of cytokines in mitogen-induced peripheral blood mononuclear cells. The evaluation of pro-inflammatory cytokines and cytotoxic molecules prove useful in the clinical identification of acutely rejecting transplant recipients and in the justification of concomitant antirejection therapy before histological diagnosis confirmation.


Subject(s)
Cytokines/blood , Cytokines/genetics , Kidney Transplantation/immunology , T-Lymphocytes, Cytotoxic/chemistry , Biomarkers/blood , Fas Ligand Protein , Gene Expression , Graft Rejection/diagnosis , Granzymes , Humans , Leukocytes, Mononuclear/chemistry , Membrane Glycoproteins/blood , Membrane Glycoproteins/genetics , Perforin , Polymerase Chain Reaction , Pore Forming Cytotoxic Proteins , RNA, Messenger/metabolism , RNA, Messenger/physiology , Serine Endopeptidases/blood , Serine Endopeptidases/genetics , Up-Regulation
2.
Environ Health Perspect ; 108(3): 205-11, 2000 Mar.
Article in English | MEDLINE | ID: mdl-10706525

ABSTRACT

We investigated whether organochlorine exposure is associated with the incidence of infectious diseases in Inuit infants from Nunavik (Arctic Quebec, Canada). We compiled the number of infectious disease episodes during the first year of life for 98 breast-fed and 73 bottle-fed infants. Concentrations of organochlorines were measured in early breast milk samples and used as surrogates to prenatal exposure levels. Immune system parameters were determined in venous blood samples collected from infants at 3, 7, and 12 months of age. Otitis media was the most frequent disease, with 80. 0% of breast-fed and 81.3% of bottle-fed infants experiencing at least one episode during the first year of life. During the second follow-up period, the risk of otitis media increased with prenatal exposure to p,p'-DDE, hexachlorobenzene, and dieldrin. The relative risk (RR) for 4- to 7-month-old infants in the highest tertile of p, p'-DDE exposure as compared to infants in the lowest tertile was 1. 87 [95% confidence interval (CI), 1.07-3.26]. The RR of otitis media over the entire first year of life also increased with prenatal exposure to p,p'-DDE (RR, 1.52; CI, 1.05-2.22) and hexachlorobenzene (RR, 1.49; CI, 1.10-2.03). Furthermore, the RR of recurrent otitis media ( [Greater/equal to] 3 episodes) increased with prenatal exposure to these compounds. No clinically relevant differences were noted between breast-fed and bottle-fed infants with regard to immunologic parameters, and prenatal organochlorine exposure was not associated with immunologic parameters. We conclude that prenatal organochlorine exposure could be a risk factor for acute otitis media in Inuit infants.


Subject(s)
Dichlorodiphenyl Dichloroethylene/adverse effects , Dieldrin/adverse effects , Environmental Exposure/adverse effects , Hexachlorobenzene/adverse effects , Infections/ethnology , Infections/immunology , Insecticides/adverse effects , Inuit/statistics & numerical data , Maternal Exposure/adverse effects , Otitis Media/ethnology , Otitis Media/immunology , Acute Disease , Adult , Arctic Regions/epidemiology , Bottle Feeding/statistics & numerical data , Breast Feeding/statistics & numerical data , Dichlorodiphenyl Dichloroethylene/analysis , Dieldrin/analysis , Disease Susceptibility/ethnology , Environmental Exposure/analysis , Environmental Monitoring/methods , Epidemiological Monitoring , Female , Follow-Up Studies , Hexachlorobenzene/analysis , Humans , Incidence , Infant , Infections/blood , Infections/etiology , Insecticides/analysis , Male , Otitis Media/blood , Otitis Media/etiology , Population Surveillance , Quebec/epidemiology , Risk Factors
3.
Transplantation ; 65(2): 279-82, 1998 Jan 27.
Article in English | MEDLINE | ID: mdl-9458031

ABSTRACT

BACKGROUND: The CD8+CD38+ T-cell subset can predict progression to acquired immune deficiency syndrome among human immunodeficiency virus-positive subjects. This T-cell subset usually increases during other active viral infections (cytomegalovirus [CMV], Epstein Barr virus). We report on its usefulness in the early detection of CMV infection in kidney transplant recipients. METHODS: Quantitation of CD8+CD38+ T cells was monitored by dual-color flow cytometry analysis on 77 patients during the posttransplantation period. Seventeen of the 52 patients at risk for CMV disease (33%) had primary infection or reactivation and three patients had herpes simplex virus infection only. RESULTS: In every patient with CMV disease, high values for the CD8+CD38+ subset were detected with a 90% positive predictive value for the primary infections. Elevated values were observed at the very first clinical signs of the viral disease or within the few preceeding days. Acute rejection episodes did not provoke false-positive results. CONCLUSION: This immunologic marker is sensitive and easily obtainable on a daily basis. It may help to direct therapy during rejection or serve as a tool for early detection of clinical viral diseases.


Subject(s)
Antigens, CD , Antigens, Differentiation/analysis , CD8 Antigens/analysis , Cytomegalovirus Infections/immunology , Kidney Transplantation/immunology , NAD+ Nucleosidase/analysis , Opportunistic Infections/immunology , T-Lymphocyte Subsets/immunology , ADP-ribosyl Cyclase , ADP-ribosyl Cyclase 1 , Biomarkers/blood , Cytomegalovirus Infections/diagnosis , Flow Cytometry , Humans , Membrane Glycoproteins , Opportunistic Infections/diagnosis , Predictive Value of Tests
4.
Eur J Histochem ; 37(4): 375-80, 1993.
Article in English | MEDLINE | ID: mdl-7510544

ABSTRACT

This study describes an easy 3 step-procedure to prepare rapidly and at low cost, pure myoblast cell cultures from a normal muscle biopsy. Following collagenase and trypsin treatment of the tissue (step 1), dissociated cells were cloned at a density of 10 cells/ml in MCDB 120 medium (0.2 ml/well). Clones that grew were then tested for NCAM cell surface expression by cytofluorometric analysis (CFA) using Coulter CD56-PE monoclonal antibodies (step 2). Only those clones with more than 98% strongly labelled positive cells were expanded (step 3) for further trials in cell transfer therapy for dystrophic patients. Visualization of the pattern of NCAM expression was performed by immunoperoxidase assay, while the potential ability to form myotubes was confirmed by the observation of their formation within a period of 1 to 2 weeks. The 65% of the CD56+ clones in CFA were the same clones that proved to be myogenic with positive immunoperoxidase assay and myotube formation. This method avoids the fastidious and costly approach of cell sorting (whenever available), avoids contamination hazards due to many manipulations of the clones. Moreover this approach leads to a pure myoblast population free of any contaminating fibroblast which could contribute to connective tissue implement already deleterious in dystrophic patients.


Subject(s)
Cell Adhesion Molecules, Neuronal/biosynthesis , Cell Separation/methods , Clone Cells/cytology , Muscles/cytology , Muscular Dystrophies/therapy , Antibodies, Monoclonal/immunology , Cell Adhesion Molecules, Neuronal/immunology , Cell Membrane/metabolism , Clone Cells/transplantation , Humans , Immunoenzyme Techniques , Muscles/metabolism , Muscles/transplantation
7.
Mol Cell Endocrinol ; 62(1): 69-78, 1989 Mar.
Article in English | MEDLINE | ID: mdl-2545486

ABSTRACT

The metabolic effects of atrial natriuretic peptide (ANP) have not been widely investigated. Since adipocyte cells represent a model system extensively used to examine the metabolic actions of many peptide hormones, we sought to establish whether ANP could bind to adipocyte membranes, alter cyclic nucleotide metabolism, and affect spontaneous or hormone-stimulated lipolysis. Using in vitro autoradiographic techniques, radiolabelled ANP was found to bind specifically to mammary gland fat cells. Additionally, endogenous ANP-like immunoreactivity could be localized in the plasma membrane compartment and cytoplasmic matrix of fat cells, but not in fat vacuoles. [125I]ANP bound to single high affinity sites (Kd = 0.72 nM) in fat cell membranes. The binding was rapid (equilibrium within 1 min at 25 degrees C) and specific. The atrial peptide was capable of stimulating a time- and concentration-dependent increase in cGMP accumulation in isolated adipocytes, but had no effect on spontaneous or stimulated [-)-isoproterenol, ACTH, forskolin) cAMP formation. ANP did not alter the increase in glycerol production stimulated by l-epinephrine in isolated fat cells. While i.v. infusion of ANP stimulated a marked increase in circulating levels of cGMP, the atrial peptide did not alter plasma triglyceride levels. These data demonstrate the presence of specific ANP binding sites on adipocyte membranes and internalization of ANP-associated immunoreactivity. These receptors are biochemically functional given the ability of ANP to augment cGMP formation. The peptide, however, does not exert an action on adipocyte lipolysis. Adipocytes, therefore, represent an ANP target tissue in which the physiological action of the peptide is yet to be defined.


Subject(s)
Adipose Tissue/metabolism , Atrial Natriuretic Factor/metabolism , Adipose Tissue/cytology , Animals , Atrial Natriuretic Factor/pharmacology , Cell Membrane/metabolism , Cell Separation , Cyclic AMP/metabolism , Cyclic GMP/metabolism , Female , Immunohistochemistry , Lipolysis/drug effects , Male , Rats , Rats, Inbred Strains
8.
Biol Cell ; 65(2): 181-8, 1989.
Article in English | MEDLINE | ID: mdl-2525411

ABSTRACT

Internalization of 125I-labelled atrial natriuretic peptide ([ 125I]ANP) by rat adrenal glomerulosa cells in vivo was investigated by means of an ultrastructural autoradiographic approach. One to 30 min after IV injection of [125I]ANP, silver grains were found, at the light microscope level, over all glomerulosa cells; coinjection of 20 micrograms of unlabelled ANP inhibited this binding by 64%. At the electron microscope level, the time-course study indicated maximal silver grain densities in plasma membranes 1 min after IV injection; grains were detected in mitochondria (external membranes and matrix) 2 min after injection, with maximal labelling at 15 min. The cytoplasmic matrix was labelled only 30 min after injection. During the time-course, labelling of nuclei, Golgi apparatus, and lysosomes was minimal. The data suggest that after binding to plasma membranes ANP is rapidly internalized and distributed within glomerulosa cells. The association of radioactivity with mitochondria suggests that ANP may have intracellular sites of action complementary to those on plasma membranes.


Subject(s)
Adrenal Cortex/metabolism , Atrial Natriuretic Factor/pharmacokinetics , Adrenal Cortex/ultrastructure , Animals , Male , Microscopy, Electron , Rats , Rats, Inbred Strains
9.
Biochem Biophys Res Commun ; 156(2): 1000-6, 1988 Oct 31.
Article in English | MEDLINE | ID: mdl-2847709

ABSTRACT

Binding activities and metabolic effects of atrial natriuretic peptide (ANP) were examined in rat brown fat. ANP binding sites were identified in thin sections of brown adipose. Binding of [125I]-ANP to brown fat membranes was specific, saturable and time-dependent. A single class of high affinity sites (Kd, 1.7 nM; Bmax, 226 fmol/mg protein) was present. ANP increased cGMP synthesis in isolated cells (EC50, 2.5 x 10(-9) M), but did not alter epinephrine-stimulated glycerol production. The results demonstrate the presence of specific ANP receptors in brown fat, activation of which increases cGMP formation; however, since neither ANP nor cGMP affect lipolysis, the biological importance of these receptors remains unknown.


Subject(s)
Adipose Tissue, Brown/metabolism , Receptors, Cell Surface/metabolism , Adipose Tissue, Brown/drug effects , Animals , Atrial Natriuretic Factor/metabolism , Atrial Natriuretic Factor/pharmacology , Cell Membrane/metabolism , Cyclic GMP/biosynthesis , Epinephrine/pharmacology , Glycerol/metabolism , Kinetics , Lipolysis/drug effects , Rats , Rats, Inbred Strains , Receptors, Atrial Natriuretic Factor , Tissue Distribution
10.
Endocrinology ; 123(1): 149-58, 1988 Jul.
Article in English | MEDLINE | ID: mdl-2838252

ABSTRACT

Some, though not all studies, have indicated that atrial natriuretic peptide (ANP) can bind to adrenal medullary cells. ANP-like immunoreactivity (ANP-LI) has also been identified in catecholamine-secreting cells. Together, these findings suggest that ANP may be taken up and/or synthesized in the adrenal medulla. The present study was designed to ascertain, by in situ hybridization, whether adrenal chromaffin cells could synthesize ANP, to define by an in vivo ultrastructural autoradiographic approach, whether ANP could, in fact, bind to rat adrenal medulla cells, to determine whether there was a cellular [noradrenaline (NA) vs. adrenaline (A)] selectivity in the binding process, and to establish whether extracellular [125I]ANP could be internalized by these cells. The cellular and subcellular distribution of endogenous ANP-LI was also investigated in both cell types by cryoultramicrotomy and immunocytochemical approaches. The in situ hybridization studies indicate the presence of mRNA to ANP in about 15% of adrenal medullary cells. Intravenous injection of [125I]ANP resulted in a 3-fold, preferential and specific radiolabeling of A-as compared to NA-containing cells. In A-containing cells, plasma membranes were significantly labeled 2 and 5 min post injection; cytoplasmic matrix, mitochondria, and secretory granules throughout the time course studied (1-30 min post injection). Lysosomes, rough endoplasmic reticulum, Golgi apparatus, and nuclei were not labeled. ANP-LI was identified in both NA- and A-containing cells; in the former, it was almost exclusively localized in secretory vesicles, in the latter it was detected in plasma membranes, cytoplasmic matrix, nuclear euchromatin, some mitochondria and relatively fewer granules than in NA-containing cells. The findings suggest that ANP may be synthesized primarily in NA-containing cells and that A-containing cells primarily bind and internalize the extracellular (endogenous or exogenous) atrial peptide. The data suggest that ANP secreted by adrenal medullary chromaffin cells may have distal paracrine actions or interactions with coreleased catecholamines and neuropeptides. Binding and internalization may reflect an action of ANP on the secretory function of A-containing cells.


Subject(s)
Adrenal Medulla/metabolism , Atrial Natriuretic Factor/metabolism , Receptors, Cell Surface/metabolism , Adrenal Medulla/ultrastructure , Animals , Atrial Natriuretic Factor/biosynthesis , Atrial Natriuretic Factor/genetics , Autoradiography , Iodine Radioisotopes , Kinetics , Male , Microscopy, Electron , Nucleic Acid Hybridization , RNA, Messenger/genetics , Rats , Rats, Inbred Strains , Receptors, Atrial Natriuretic Factor , Subcellular Fractions/metabolism
11.
Am J Physiol ; 254(3 Pt 1): E301-9, 1988 Mar.
Article in English | MEDLINE | ID: mdl-2964789

ABSTRACT

Atrial natriuretic factor (ANF) binding sites have been recently demonstrated to be present in exocrine pancreas by an in vitro autoradiographic approach. An autoradiographic study was carried out to identify the exocrine cells containing ANF binding sites and to monitor the fate of 125I-labeled ANF in acinar cells after removal of pancreas at specific time intervals (1-30 min) after intravenous administration. At the light microscopic level, silver grains were found over acinar and centroacinar cells. Concomitant injection of an excess of unlabeled ANF inhibited the binding of labeled peptide by approximately 60%. At the electron microscopic level, the time-course study in acinar cells has revealed that of the cell compartments examined, plasma membrane, Golgi apparatus, mitochondria, and zymogen granules, the nucleus had distinct labeling patterns. Plasma membrane was maximally labeled 1 and 2 min after injection with 125I-ANF. Golgi apparatus was significantly labeled from 2 to 30 min after injection, mitochondria from 1 to 30 min after injection, zymogen granules at 1 and 15 min, and the nucleus only at 30 min. The lysosomal compartment was not labeled during the 30-min observation period. These results suggest that after binding to the plasma membrane, ANF is rapidly internalized and distributed to the intracellular organelles as a function of time. Labeling of the zymogen granules suggests that they may bind ANF and that the atrial peptide may be secreted by acinar cells. The significance of association of radioactivity with mitochondria and nuclei remains to be elucidated but may represent intracellular sites of action of ANF complementary to those on plasma membranes.


Subject(s)
Atrial Natriuretic Factor/metabolism , Pancreas/metabolism , Animals , Autoradiography , Iodine Radioisotopes , Male , Microscopy, Electron , Pancreas/cytology , Pancreas/ultrastructure , Rats , Rats, Inbred Strains
12.
Mol Cell Endocrinol ; 55(2-3): 219-31, 1988 Feb.
Article in English | MEDLINE | ID: mdl-2833418

ABSTRACT

Atrial natriuretic factors (ANF) are a family of peptides originally identified in atrial cardiocytes and having natriuretic, diuretic and vasorelaxatory properties. ANF was recently reported to be synthesized in anterior pituitary cells. In the current study, the cell-specific sites of binding and internalization of ANF in the adenohypophysis, a site of ANF action, were investigated. By in vitro autoradiographic techniques, [125I]ANF was found to bind specifically to both anterior and posterior lobes of the pituitary, but not to the intermediate lobe. 5 min following intravenous injection of [125I]ANF, radiolabel was detected by ultrastructural autoradiography on gonadotrophs, corticotrophs and lactotrophs, but not thyrotrophs or somatotrophs. Label was found at both the plasma membrane level and cytoplasmic matrix, but not in the nuclei, of all three cell types. The cellular and subcellular localization of endogenous ANF-like immunoreactivity was also investigated. Specific immunoreactivity was also detected in gonadotrophs, corticotrophs and some lactotrophs. At the subcellular level in all three cell types, immunoreactivity was localized in the cytoplasmic matrix, on secretory granule membranes, and in mitochondria. Less dense staining was observed in nuclear euchromatin; endoplasmic reticulum and Golgi apparatus were not labelled in any of the cells. The data suggest that notwithstanding their reported ability to synthesize ANF, exogenous ANF can also bind and be internalized by adenohypophyseal gonadotrophs; the binding and presence of endogenous ANF-like immunoreactivity in corticotrophs and lactotrophs suggest that these cells may be sites of action for the atrial peptide.


Subject(s)
Atrial Natriuretic Factor/metabolism , Pituitary Gland, Anterior/metabolism , Receptors, Cell Surface/metabolism , Animals , Atrial Natriuretic Factor/biosynthesis , Cell Membrane/metabolism , Male , Microscopy, Electron , Pituitary Gland, Anterior/cytology , Pituitary Gland, Anterior/ultrastructure , Rats , Receptors, Atrial Natriuretic Factor
13.
Can J Physiol Pharmacol ; 66(1): 32-7, 1988 Jan.
Article in English | MEDLINE | ID: mdl-2453261

ABSTRACT

Pancreatic acinar cells do not contain depolarization-sensitive calcium channels. Nonetheless, in the current study, the calcium channel activator, BAY-K-8644, was found to stimulate a time- and concentration-dependent increase in the spontaneous release of amylase. Secretion was dependent on the presence of extracellular calcium in the incubation medium. Racemic BAY-K-8644 and (or) its S(-)optical isomer did not enhance the secretory response to either carbachol or cholecystokinin octapeptide; however, when co-applied with either phorbol ester, vasoactive intestinal peptide, or forskolin, they potentiated amylase secretion. Nifedipine and the R(+)isomer of BAY-K-8644, which are both calcium channel antagonists, did not alter basal or forskolin-stimulated amylase secretion, and [3H]nitrendipine did not bind to acinar cell membranes. Neither atropine nor dibutyryl cGMP, inhibitors of cholinergic and cholecystokininergic receptors, respectively, affected BAY-K-8644-induced amylase secretion. While BAY-K-8644 stimulated concentration-dependent cGMP synthesis in acinar cells, it had no effect on basal or forskolin-stimulated cAMP formation. The data suggest that BAY-K-8644 may bind to acinar cell sites that are not functional calcium channel proteins but are coupled nevertheless to the secretory response, and that calcium channel antagonists do not bind to these sites. The mechanism of the secretagogue action of BAY-K-8644 remains to be elucidated.


Subject(s)
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester/pharmacology , Amylases/metabolism , Pancreas/drug effects , Animals , Calcium/metabolism , Calcium/pharmacology , In Vitro Techniques , Ion Channels/metabolism , Kinetics , Pancreas/metabolism , Rats , Stereoisomerism
14.
J Rheumatol ; 14(4): 656-61, 1987 Aug.
Article in English | MEDLINE | ID: mdl-3668972

ABSTRACT

We have investigated protein synthesis and release by polymorphonuclear leukocytes (PMN) to compare the protein biosynthetic activity of peripheral blood PMN and inflammatory synovial fluid (SF) PMN from patients with inflammatory arthropathies. We analyzed and compared the protein profiles produced by these cells, using patient matched peripheral blood and SF PMN as well as peripheral blood PMN from normals. Twenty-five patients with either rheumatoid arthritis, psoriatic arthritis or gout were studied. Fluorographs of SDS-polyacrylamide slab gels, performed using cell supernatants from metabolically labelled cells, revealed an increased release of de novo synthesized proteins by inflammatory SF PMN compared to peripheral blood PMN. Under reducing conditions, 4 clearly distinguishable high molecular mass products were observed (Mr 230,000, 185,000, 170,000 and 95,000). Two of the protein bands were found to be gelatin binding (Mr 230,000 and Mr 95,000). By Western blot, the Mr 230,000 protein was found to be fibronectin and the Mr 95,000 protein was shown to be identical to a recently described gelatinase. Thus, the activation of PMN in inflammation is accompanied by an increased release of a number of de novo synthesized proteins, including fibronectin. Our studies directly pertain to the in vivo inflammatory process since the PMN were not activated artificially in vitro.


Subject(s)
Arthritis/blood , Fibronectins/biosynthesis , Gout/blood , Neutrophils/metabolism , Psoriasis/blood , Arthritis, Rheumatoid/blood , Electrophoresis, Polyacrylamide Gel , Humans , Synovial Fluid/cytology
15.
Pancreas ; 2(4): 404-13, 1987.
Article in English | MEDLINE | ID: mdl-2819860

ABSTRACT

Atrial natriuretic factor (ANF) binding sites in adult rat exocrine pancreas were studied by autoradiography using slide-mounted frozen tissue sections with mono-iodinated ANF (101-126) as the tracer. Radiolabel was displaced by unlabeled atrial peptide (IC50 = 2 X 10(-11) M). High specific labeling was found in pancreatic acini. The presence of endogenous ANF has also been demonstrated in the exocrine pancreas by immunocytochemistry on ultra-thin sections obtained by cryoultramicrotomy. ANF-like immunoreactivity was found in acinar and centro-acinar cells as well as cells of the intercalated duct. For these cells, immunostaining was observed at the plasma membrane level, in the cytoplasm and nucleus. In the cytoplasm, ANF-like immunoreactivity was observed in the cytoplasmic matrix, mitochondria, and zymogen granules. In the nucleus, ANF-like immunoreactivity was distributed in the vicinity of the heterochromatin region primarily in the euchromatin. It was also detected in the plasma membrane of microvilli of acinar and duct cells, and in the lumen of secretory ducts. In centro-acinar cells, the reaction product was also found sparsely at the nuclear envelope. No immunoreactivity was observed when anti-human ANF serum preincubated with rat ANF was used. No modifications were observed when this antiserum was preincubated with heterologous peptides (NPY, CRF, GRF, TRH, somatostatin). These data provide autoradiographic evidence of ANF binding sites, indicate the presence of this peptide in acinar and centro-acinar cells as well as cells of the intercalated duct, and immunocytochemical evidence for the internalization of endogenous ANF by exocrine pancreas.


Subject(s)
Atrial Natriuretic Factor/analysis , Pancreas/analysis , Receptors, Cell Surface/analysis , Animals , Autoradiography , Binding Sites , Histocytochemistry , Male , Microscopy, Electron , Pancreas/ultrastructure , Rats , Rats, Inbred Strains , Receptors, Atrial Natriuretic Factor
16.
Biol Cell ; 59(3): 233-8, 1987.
Article in English | MEDLINE | ID: mdl-3038234

ABSTRACT

Because extended exposure of AtT-20 corticotropin-secreting cells to atrial natriuretic factor (ANF) results in a desensitization of ANF-induced cGMP synthesis, we sought to establish whether pretreatment of AtT-20 cells with the atrial peptide also led to an internalization process. In fact, by coupling an ultrastructural approach to cryoultramicrotomy, ANF-immunoreactivity was detected at both the plasma membrane level and at intracellular sites in AtT-20 cells. Internalization was observed within 5 min at which time labelling was observed in the plasma membrane level, in vacuole-like structures in close proximity to the plasma membrane, in cytoplasmic matrix and sometimes in mitochondria. After 30 min exposure Golgi apparatus, mitochondria and nuclear euchromatin were also labelled. Following 1-4 hr, labelling in other cell compartments, e.g. lysosomal, was increased, while it was reduced in plasma membranes and vacuole-like structures. Secretory granules and endoplasmic reticulum were not labelled throughout the time course. Extraction of a intracellular [125I] ANF from AtT-20 cells following 4 hr incubation suggested that about 90% of the peptide was intact. The data suggest that internalization of ANF may serve to terminate the biological response associated with ANF receptor activation; subcellular distribution of internalized, intact ANF suggests that the peptide may have other, as yet unidentified, intracellular actions.


Subject(s)
Adrenocorticotropic Hormone/metabolism , Atrial Natriuretic Factor/metabolism , Animals , Biological Transport , Cell Line , Cell Membrane/metabolism , Cell Membrane/ultrastructure , Histocytochemistry , Immunoassay , Mitochondria/metabolism , Mitochondria/ultrastructure
17.
Biochem J ; 240(3): 863-9, 1986 Dec 15.
Article in English | MEDLINE | ID: mdl-2950853

ABSTRACT

Dialysis of demembranated bull spermatozoa against a low-salt buffer resulted in the solubilization of the outer dynein arms and 15-25% of the total ATPase activity. Low-salt extracts contained three high-Mr peptides with Mr values above 300,000. The ATPase activity was associated with two particles sedimenting at 19 S and 12 S. The heavier particle contained two major high-Mr peptides with Mr values above 300,000, one major and one minor intermediate peptides with Mr values of 91,000 and 140,000 respectively and lower-Mr peptides. The 12 S particle contained one high-Mr peptide positioned between the two heavy peptides of the 19 S particle. Even though the peptide compositions of these two particles were different, the enzymic properties of their ATPases were similar. Both particles hydrolysed in the following preference order: ATP greater than CTP greater than UTP greater than ITP greater than GTP. ATPase activities were not affected by ouabain and oligomycin but were inhibited by vanadate, erythro-9-[3-(2-hydroxynonyl)]adenine and EDTA. Enzyme activities were dependent on the presence of a bivalent cation with the following preference order: Mn2+ greater than Mg2+ greater than Ca2+ greater than Ni2+. Optimal activity was observed between pH 6.5 and 9.5. The Km for ATP ranged from 40 to 50 microM for both 19 S and 12 S ATPases. These results suggest that the 12 S and 19 S particles are dyneins from outer dynein arms.


Subject(s)
Adenosine Triphosphatases/metabolism , Dyneins/metabolism , Spermatozoa/enzymology , Animals , Antimetabolites/pharmacology , Cations, Divalent/pharmacology , Cattle , Dyneins/isolation & purification , Electrophoresis, Polyacrylamide Gel , Kinetics , Male , Microscopy, Electron , Nucleotides/pharmacology , Spermatozoa/drug effects , Spermatozoa/ultrastructure
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