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1.
Commun Biol ; 5(1): 1231, 2022 11 12.
Article in English | MEDLINE | ID: mdl-36371461

ABSTRACT

Cell-cell communication and physical interactions play a vital role in cancer initiation, homeostasis, progression, and immune response. Here, we report a system that combines live capture of different cell types, co-incubation, time-lapse imaging, and gene expression profiling of doublets using a microfluidic integrated fluidic circuit that enables measurement of physical distances between cells and the associated transcriptional profiles due to cell-cell interactions. We track the temporal variations in natural killer-triple-negative breast cancer cell distances and compare them with terminal cellular transcriptome profiles. The results show the time-bound activities of regulatory modules and allude to the existence of transcriptional memory. Our experimental and bioinformatic approaches serve as a proof of concept for interrogating live-cell interactions at doublet resolution. Together, our findings highlight the use of our approach across different cancers and cell types.


Subject(s)
Transcriptome , Triple Negative Breast Neoplasms , Humans , Microfluidics , Gene Expression Profiling/methods , Gene Expression Regulation
2.
Clin Exp Metastasis ; 37(3): 413-424, 2020 06.
Article in English | MEDLINE | ID: mdl-32335861

ABSTRACT

Patient-derived orthotopic xenograft (PDOX) models have been verified as a useful method for studying human cancers in mice. Previous studies on the extent of metastases in these models have been limited by the necessity of welfare euthanasia (primary tumors reaching threshold size), at which point metastases may only be micrometers in diameter, few in number, and solely identified by step-sectioning of formalin-fixed paraffin-embedded tissue. These small micro-metastases are less suitable for many downstream molecular analyses than macro-metastases. Resection of the primary tumor by survival surgery has been proven to allow further time for metastases to grow. Although PDOX models of triple-negative breast cancer (TNBC) shed circulating tumor cells (CTCs) into the bloodstream and metastasize, similar to human TNBC, little data has been collected in these TNBC PDOX models regarding the association between CTC characteristics and distant metastasis following excision of the primary tumor xenograft. This study assembles a timeline of PDOX tumor shedding and metastatic tumor progression before and after tumor excision surgery. We report the ability to use tumorectomies to increase the lifespan of TNBC PDOX models with the potential to obtain larger metastases. CTC clusters and CTCs expressing a mesenchymal marker (vimentin) were associated with metastatic burden in lung and liver. The data collected through these experiments will guide the further use of PDOX models in studying metastatic TNBC.


Subject(s)
Liver Neoplasms/secondary , Lung Neoplasms/secondary , Neoplasm Micrometastasis/pathology , Triple Negative Breast Neoplasms/pathology , Xenograft Model Antitumor Assays/methods , Animals , Cell Count , Disease Progression , Female , Humans , Mice , Neoplastic Cells, Circulating/pathology , Triple Negative Breast Neoplasms/surgery , Vimentin/metabolism
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