[The effect of three different hypocaloric diets on oxidative phosphorylation and activity of enzymatic antioxidant system in rat liver mitochondria].

Thyroid status, liver mitochondrial functional state and activity of enzymatic antioxidant system after 2-months maintenance of rats on three different hypocaloric diets were investigated. It was established that the level of calorie restriction was directly proportional to triiodothyronine content in blood and to decrease of phosphorylating oxidation intensity (with its efficacy preservation) and was inversely proportional to increase of Se-dependent glutathione peroxidase activity.

[Effect of biologically active substances of animal and plant origin on prooxidant-antioxidant balance in rats with experimental prostatic hyperplasia].

The effect of biologically active complexes of animal (prostatilen) and plant (permixon) origin on physiological indices of prostate and prooxidant-antioxidant balance in prostate and blood was studied in rats with the hyperprolactinemia-induced prostatic hyperplasia. It was shown that both prostatilen (1 mg of the total peptides per kg) and permixon (100 mg of Serenoa repens extract per kg) prevent increase in the prostate mass and volume, in the content of lipid hydroperoxides, and in the glutathione peroxidase activity in prostate. Prostatilen, in contrast to permixon, normalized the content of lipid hydroperoxides (increased under hyperplazia conditions) and increases glutathione peroxidase activity (reduced under hyperplazia conditions).

[Effect of cinnarizine on the brain mitochondrial oxidative system, antioxidant blood activity, and the rat behavior in hypoxia]. / Vliianie tsinnarizina na mitokhondrial'nuiu sistemu okisleniia mozga, antiokislitel'nuiu aktivnost' krovi i povedenie krys pri gipoksii.

The effect of cinnarizine on the functional state of brain mitochondria, the activity of blood antioxidant system, and the behavior of rats was studied under model hypoxic hypoxia conditions. A four-day treatments with cinnarizine (50 mg/kg, twice per day via a gastric tube) prevents the hypoxic brain edema development, restores NAD+ dependent oxidation of a succinate substrate, normalizes emotional-exploratory activity, and causes hyperlocomotion of the experimental animals, while not influencing a high level of activity of the blood antioxidant system.

[Features of ischemic injuries to membranes and activation of lipid peroxidation in the myocardium of rats of various ages]. / Osobennosti ishemicheskogo povrazhdeniia membran i aktivatsiia perekisnogo okisleniia lipidov v miokarde krys raznogo vozrasta.

The model of interrupted ischemia of the isolated heart has been used to show that the yield of lactate dehydrogenase into perfusate in experiments on old rats is described by more sharply pronounced S-like dependence than that in young immature animals. The activation of lipid peroxidation precedes the sarcolemma damage and is relatively more manifested in rats of middle and old age. The respiratory control of mitochondria (in homogenates) decreases considerably already 20 min after ischemia, however mitochondria retain their ability to synthesize ATP 3 h later as well. The causal-effect relationship between the activation of lipid peroxidation and partial disturbance of the mitochondrial function remains unclear.

[Prolonged storage of "firmly conjugated" mitochondria in liquid nitrogen]. / Dlitel'noe khranenie "prochnosopriazhennykh" mitokhondrii v zhidkom azote.

A method is described to obtain mitochondria whose matrix at the stage of organella washing is saturated by dimethyl sulphoxide in the concentration necessary to prevent the cryodamage of membranes. Such mitochondria, being frozen and stored in liquid nitrogen, retain the value of a respiratory control of the coefficient ADP/O and the respiratory chain activity for 1.0-1.5 and more months.

[Structural state of rat liver mitochondrial and microsomal membranes in aging and after treatment with thyroxine]. / Strukturnoe sostoianie membran mitokhondrii i mikrosom pecheni krys pri starenii i pod deistviem tiroksina.

Rotation mobility of stearic acid spin labelling in mitochondria and microsomes of 24 months old rats is shown to decrease in comparison with that of 1 month rats. This decrease correlates with an increase in the free cholesterol content in membranes. The phospholipid composition is practically unchanged. With age the osmoresistance of organelles decreases and the cryoresistance increases, supporting indirectly the existence of structural changes in membranes with ageing. The most pronounced activation of NADPH-cytochrome C reductase in the microsomal membrane after the treatment with triton X-100 is observed in case of old animals. The thyroxine treatment of old rats causes a "rejuvenative" effect on the structural state of mitochondrial and microsomal membranes, evidencing for the important role of hormonal regulation in mechanisms of their age rearrangement.

[ATP-synthetase activity, respiration and cytochromes of rat heart mitochondria in aging and hyperthyroidism]. / ATP-sintetaznaia aktivnost', dykhanie i tsitokhromy mitokhondrii serdtsa krys pri starenii i gipertireoze.

The ATP-synthetase activity, the rate of oxygen uptake under different metabolic conditions, the tightness of coupling of respiration to oxidative phosphorylation and the cytochrome contents in heart mitochondria of rats from different age groups were studied under normal conditions and in hyperthyroidism. It was found that heart mitochondria of aged animals did not practically differ in terms of their functional activity from those of the young animals. Administration of thyroxin to the animals from all age groups produced no significant effects on the state of mitochondria, increasing the rate of ATP synthesis on alpha-glycerophosphate, which was especially well-pronounced in aged animals, and the cytochrome content in 1-month-old rats.