ABSTRACT
Investigated the role of hypercapnic component in the mechanism of activation of HIF-1α and increase the synthesis of erythropoietin in the combined and the isolated impact of hypoxia and hypercapnia. It was found that the content of proteins of interest significantly increases both in isolated hypoxia and hypercapnia, and at their combined effect. Moreover, the hypercapnic hypoxia causes maximum activation of the synthesis and accumulation of erythropoietin HIF-1α, and permissive hypercapnia enhances their content more than hypoxic exposure.
Subject(s)
Brain/metabolism , Erythropoietin/biosynthesis , Hypercapnia/metabolism , Hypoxia, Brain/metabolism , Hypoxia-Inducible Factor 1, alpha Subunit/biosynthesis , Nerve Tissue Proteins/biosynthesis , Animals , Brain/pathology , Hypercapnia/pathology , Hypoxia, Brain/pathology , Male , RatsABSTRACT
Studied the role of VEGF, HSP-70 and S-100B in potentiating hypercapnia neuroprotective effect of hypoxia. Demonstrated that neuroprotective effects when exposed hypercapnic hypoxia-mediated protein synthesis increased S-100B, mainly due to the action of carbon dioxide, and not oxygen deficiency. Neuroprotective effects of HSP-70 due to hypoxia, but the combined effect of hypoxia and hypercapnia gives a significant increase in the synthesis of HSP-70 in comparison with the isolated effect of hypoxia. Vascularization activated equally as hypoxia and hypercapnia, without adding significant effects in combination. This suggests dominant effect hypercapnia, hypoxia compared in neuroprotection mechanisms related to protein S-100B, but not the protein VEGF, hypercapnia and potentiate the neuroprotective efficacy of hypoxia-related protein HSP-70.
Subject(s)
HSP70 Heat-Shock Proteins/blood , Hypercapnia/blood , Hypoxia/blood , S100 Calcium Binding Protein beta Subunit/blood , Vascular Endothelial Growth Factor A/blood , Animals , Hemodynamics , Hypercapnia/physiopathology , Hypoxia/physiopathology , Male , Rats , Rats, WistarABSTRACT
The segregating maize population (GK26 x Mo17)F2 has been used for identification of ISSR markers able to reveal a significant difference between alleles by a quantitative index. Confidence ranges have been determined for variation in 17 quantitative traits. Variations in the traits under study correlate with the inheritance of 16 marker loci have been found. The nature of these correlations and the possibility of chromosomal mapping of genetic markers are discussed.
Subject(s)
Genetic Markers , Polymerase Chain Reaction/methods , Quantitative Trait, Heritable , Zea mays/genetics , Crosses, Genetic , Genetic Variation , Phenotype , Polymorphism, Genetic , Recombination, Genetic , Repetitive Sequences, Nucleic AcidABSTRACT
Methods of ISSR- and RAPD-analyses were used for marking quantitative trait loci (QTLs) determining the development of some morphological and biological traits in maize. Specificity of marker locus alleles was established for certain levels of polygenic trait phenotype manifestation. Criteria of marker locus informativity are discussed. A possibility of marker-assisted selection for valuable genotypes with desired for breeding trait values was demonstrated.
Subject(s)
DNA, Plant/genetics , Quantitative Trait Loci/genetics , Zea mays/genetics , Alleles , Genetic Markers/genetics , Genetic Variation , Genotype , Models, Genetic , Phenotype , Polymerase Chain ReactionABSTRACT
In experiments with partial hepatectomy in rats, the application of cresacin was shown to potentiate processes of hepatocyte regeneration, to increase high-energy compounds therein, and to accelerate some phases of mitotic cycle. These processes occur along with an inhibition of lipid peroxidation in hepatocytes, a decrease in the rate of transmembrane oxygen transport in mitochondria, and a reduction of cytochrome oxidase amount. It was concluded that cresacin directly stimulates diverse links of metabolic pathway.
Subject(s)
Acetates/pharmacology , Ethanolamines/pharmacology , Liver Regeneration/drug effects , Animals , Drug Combinations , Energy Metabolism/drug effects , Hepatectomy , Liver/drug effects , Liver/metabolism , Liver/ultrastructure , Liver Regeneration/physiology , Luminescent Measurements , Male , Oxidation-Reduction/drug effects , Rats , Stimulation, ChemicalABSTRACT
It was shown that isoproxihermatran (IPH) (40 mg/kg) administered intraperitoneally in rats with lobectomy of heparin stimulates development of hepatocytes' dimensions and decrease of oxygen transmission velocity through the mitochondrial membranes in this cells. Antioxidant properties of IPH have been detected. Stimulating activity of IPH at heparin secretion of bile.
Subject(s)
Liver Regeneration/drug effects , Liver/drug effects , Nitriles/pharmacology , Organometallic Compounds/pharmacology , Animals , Bile/metabolism , Cell Size/drug effects , Electron Transport/drug effects , Interphase/drug effects , Lipid Peroxidation/drug effects , Liver/cytology , Liver/metabolism , Male , Mitochondria, Liver/drug effects , Mitochondria, Liver/metabolism , Mitosis/drug effects , Oxygen Consumption/drug effects , RatsSubject(s)
Acetates/pharmacology , Ethanolamines/pharmacology , Liver Regeneration/drug effects , Animals , Drug Combinations , Hepatectomy , Lipid Peroxidation/drug effects , Liver/drug effects , Liver/ultrastructure , Luminescent Measurements , Male , Microscopy, Electron , Mitochondria, Liver/drug effects , Mitochondria, Liver/metabolism , Mitosis/drug effects , Rats , Stimulation, ChemicalABSTRACT
Stimulating effect of 1-ethoxysilatrane on healing processes of experimental stomach ulcers was studied in rats. 1-ethoxysilatrane inhibited lipid peroxidation in blood and stomach wall tissues, interacted with artificial membranes, and affected the rate of mitochondrial respiration in liver. A hypothesis is put forward on the membranotropic effect of 1-ethoxysilatrane which underlies its ulcerostatic effect.
