[A morphometric analysis of tissue homeostasis in peptic ulcer patients during treatment with antihistamine preparations]. / Morfometricheskii analiz tkanevogo gomeostaza u bol'nykh iazvennoi bolezn'iu pri lechenii antigistaminnymi preparatami.

The authors carried out a comparative examination of 68 patients with duodenal ulcer receiving monotherapy with cithemidine and a combination of cithemidine and dimedrol. Clinical data indicate that combined use of H1- and H2-histamine blockaders produce a reliable therapeutic effect. Morphological investigations of biopsies from the periulcerous region and morphometry of cellular and noncellular structures showed that the mechanism of this effect consists in strengthening of the mast cell apparatus adding the excess of tissue histamine.

[Pathogenesis and classification of peptic ulcer]. / O patogeneze i klassifikatsii iazvennoi bolezni.

The authors present data on the mechanism of ulcer formation in stomach and duodenum in patients with ulcer disease. The pathogenesis of ulcer disease is complicated and far from being solved. It is suggested that ulcerogenesis of ulcer disease is only a link of the pathogenesis of ulcer disease. Ulcer defect of the gastric and duodenal mucosa is the main morphological sign of ulcer disease but not the complete symptom complex of systemic disorders in the body which along with ulcerogenesis are the essence of ulcer disease. A working classification of ulcer disease is presented.

[Physico-chemical study of the heterogeneous population of opioid receptors]. / Fiziko-khimicheskoe issledovanie geterogennoi populiatsii opioidnykh retseptorov.

A physico-chemical analysis of the heterogenous population of opioid receptors was carried out. A new difference approach to the analysis of heterogenous receptor systems was developed. This procedure permits to estimate even in high stringency conditions of parameters of three or more types of receptors from the binding isotherms or competitive replacement curves as well as to determine the total receptor concentration in the system. A DELTA computer program based on this difference approach has been developed. The experimental results obtained through the use of the difference technique led to a kinetic model of interaction between morphine and D-Ala2, D-Leu5-enkephalin with rat brain receptors. This model based on the interaction of each ligand with three types of specific binding sites can be used for the determination of kinetic and thermodynamic parameters.

[Kinetics of fumarate hydratase reaction catalyzed by free cells of Escherichia coli]. / Kineticheskoe izuchenie fumarat-gidrataznoi reaktsii, kataliziruemoi svobodnymi kletkami Escherichia coli.

The kinetics of the fumarate hydratase (fumarase) reaction catalyzed by the cells of E. coli strain 85 at high concentrations of the substrate (potassium fumarate) were studied. An automatic procedure for determination of the reaction product--malonic acid--including the use of commercial malate dehydrogenase from porcine heart was developed. The fumarate activity of bacterial cells was studied at different concentrations of the substrate and at different pH values with intact and disrupted cells of E. coli 85 used as the enzyme source. The rate of the fumarase reaction in the E. coli cells was shown to depend on the diffusion and transport processes of the reagent transfer across the cell wall and the cytoplasmic membrane of bacterial cells. The pH optimum of the reaction in free E. coli cells (8-9) and the rate of malonic acid synthesis from potassium fumarate under optimal conditions, which varies within the concentration range of (6--13) x 10(-5) mkmole per mg of protein depending on the quality of cell, were determined.